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Cheebabear · 09-21-06, 23:10

I whipped up 9 1/2pint PF jars the yesterday and pc'd em for 60minutes, today I innoculated them each at four points per jar with Mazatepec spores. I used about 1 ml per jar. One ml was injected into a liquid culture for future innoculations. Got all 9 jars and the liquid culture in a closed cooler with a submersible aquarium heater in a plastic bottle. Right now it's at 82oF with 80% RH. Now... I just wait.. and wait...wait..

This will be my second time cultivating mushrooms.. although the 1st time went on for a few months because I kept making popcorn & wbs spawn jars and liquid cultures for pf cakes. Got many cakes and flushes until I started getting many many contams, which thanks to this site I found out it was the Coco Coir I was using. I can't wait to see how this strain looks firsthand.. I'm going to make as many prints as I can this round to donate to the Topia.

Hippie, I have seen how you prefer spore prints. My first round cultivating, I sterilized a small stack of 3"x5" index cards in the PC and printed onto those, folded them in half and taped the edges, vacuum sealed them in a small plastic bag with small amount of dessicant. Am I asking for trouble using the index cards? This time around I will use foil as it seems to be the preferred method by most on here.

Will post pictures once I see some myc growth.

09-21-06, 23:10	#1 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	2nd round has begun... I whipped up 9 1/2pint PF jars the yesterday and pc'd em for 60minutes, today I innoculated them each at four points per jar with Mazatepec spores. I used about 1 ml per jar. One ml was injected into a liquid culture for future innoculations. Got all 9 jars and the liquid culture in a closed cooler with a submersible aquarium heater in a plastic bottle. Right now it's at 82oF with 80% RH. Now... I just wait.. and wait...wait.. This will be my second time cultivating mushrooms.. although the 1st time went on for a few months because I kept making popcorn & wbs spawn jars and liquid cultures for pf cakes. Got many cakes and flushes until I started getting many many contams, which thanks to this site I found out it was the Coco Coir I was using. I can't wait to see how this strain looks firsthand.. I'm going to make as many prints as I can this round to donate to the Topia. Hippie, I have seen how you prefer spore prints. My first round cultivating, I sterilized a small stack of 3"x5" index cards in the PC and printed onto those, folded them in half and taped the edges, vacuum sealed them in a small plastic bag with small amount of dessicant. Am I asking for trouble using the index cards? This time around I will use foil as it seems to be the preferred method by most on here. Will post pictures once I see some myc growth. __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."