| When my FOAF inoculates, he goes in slowly at a steep angle, so the needle is actually touching the glass. Then he rotates the needle, until the hole is visible through the side of the glass (you can see if it is plugged). Then solution is added slowly, so it spreads against the glass and drips down the side. .3mL to .6mL is usually enough to drip all the way to the bottom of the jar. He stops when the liquid hits the bottom of the jar.
And yeah, practicing this with water and dummy jars is a very good idea. Especially if you have never handled needles before.
But don't worry, you'll get the hang of it!!! |