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Originally Posted by fastfred  Can you post a link or cite on that MgSO4 info?
I've read a couple abstracts that show EDTA is effective in inactivating alkaline phosphatase. EDTA is about the most effective chelating agent out there. It's also safe and makes a good preservative. I've discussed it a bit at the shroomery and I think it would make a great preservative for our needs.
Phosphatase enzymes require iron ions to do their work so EDTA is about as effective as it gets, but I'd like to hear about MgSO4 also. How is it able to inactivate phosphatases?
-FF |
Source:
Dephosphorylation of Psilocybin to Psilocin by Alkaline Phosphatase (26228) A. Horita and L.J. Weber (Introduced by T.A. Loomis)
Dept. of Phamacology, University of Washington School of Medicine Seattle)
Here are some interesting excerpts from that text
- At 37 C.(Bodytemperature) they found:
Quote:
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A rapid dephosphorylation is evident in the initial 15-30 minutes.
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The reaction proceeds at a lower rate after 30 minutes. - The activity of alkaline posphatase can be controled in different ways:
An excerpt from somewere else: -FF 
you know this *grin*
source:http://www.shroomery.org/forums/show...Number/5925358
Mg++ ions protect the enzyme from inactivation by complexing agents
Now back to Horita/Weber. They made a tests with magnesium sulfate
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Since the Mg++ ion is known to activate alkaline phosphatase its effect was also studied
with psilocybin as substrate. It was found that at concentrations of 0.35% MgSO4,
psilocin and inorganic phosphate liberated increased about 30% above controls.
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Quote:
Determinations of the sulubilities of these 2 compounds in organic solvents and in
Tyrode solution at ph 7.4 showed psilocin to be over 100 times as soluble in chloroform than was
psilocybin.(unpublished results)
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not sure but i think i have found the document at Hofmann foundation.
just started creating a document with excerpts like above. not much