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Originally Posted by lewiscarroll  Well, at least for that syringe. Only 1 out of 4 did anything so far. I'm still wondering if I did something wrong with the alcohol or needle cooling. Any rules of thumb regarding those you might share? |
I flame the needle once at the beginning of the inoculation session and wipe it with a paper towel soaked in isopropyl, then in between each jar I just wipe it with the towel. I might not even need to flame it the first time, but old habits die hard and it does make it certain to be clean.
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Originally Posted by Hippie3 well any growth shows that spores are / were in the syringes |
One spot of growth on one jar shows me there's only one syringe (out of three) that has at least a couple of viable spores in it.
My thought was that if 2 of 3 LC's showed no growth and the 3rd showed very slow growth from very few points that it would point to possible accidental sterilization, either when flaming the needle or by sitting in a hot mailbox, and the lack of contamination supports this theory IMO.
lewiscarroll: Though not an issue with this grow, contamination will pop up at some point and that's another huge advantage of doing LC's: If a syringe is contaminated or the spores were killed you only waste a jar of dilluted corn syrup or whatever instead of however many jars you would've otherwise inoculated. And mixing up BRF jars right is trickier than making liquid culture, so you'll looking for ways to store all your surplus fungi soon enough!
