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BuckarooBanzai · 10-06-09, 10:55

You can boil sterilize agar, but a PC is more certain.

I would not try to use a microwave to sterilize agar.

Boil over with agar causes a horrible mess and is very common. That is why you never fill a jar of agar to be pressure sterilized over 2/3 full.

A full jar of agar will boil over in the PC and cause an un-Godly mess.

More important than sterilizing the agar is how you pour and seal the Petri dishes. I strongly recommend Parafilm.

Here is a thread I did on making agar plates:
http://forums.mycotopia.net/agar-str...zai-style.html (Agar for the masses - Banzai Style!)

10-06-09, 10:55	#4 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,053	You can boil sterilize agar, but a PC is more certain. I would not try to use a microwave to sterilize agar. Boil over with agar causes a horrible mess and is very common. That is why you never fill a jar of agar to be pressure sterilized over 2/3 full. A full jar of agar will boil over in the PC and cause an un-Godly mess. More important than sterilizing the agar is how you pour and seal the Petri dishes. I strongly recommend Parafilm. Here is a thread I did on making agar plates: http://forums.mycotopia.net/agar-str...zai-style.html (Agar for the masses - Banzai Style!) __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)