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| Agar & Strain Isolation Making, pouring and using agar. Isolating pure sub-strains. |
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| | #1 (permalink) |
| Mycophiliac Join Date: Nov 2006
Posts: 24
![]() | DIY Agar from scratch
I was asked to post this over here at Mycotopia. I found a good deal on 3lbs of natural agar-agar. It is the lower grade agar known as Gracilaria lichenoides. The plus side is that the agar can be extracted by simple boiling. ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Last edited by Beast; 03-13-08 at 01:57. |
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| | #6 (permalink) |
| Mycophiliac Join Date: Nov 2006
Posts: 24
![]() | ![]() ![]() From what I have read. After this bout of de-watering I should be left with 20% agar. This is just the agar that set in the cupcake pans. The three gallons in one of the pictures in this thread was boiled down to just one gallon. I think most of the water was removed from that batch by boiling it. I have that gallon outside freezing now. Once that one is frozen and then thawed. I'm going to combine the two batches and boil them down together. Last edited by Beast; 03-13-08 at 01:58. |
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| | #8 (permalink) |
| Mycophiliac Join Date: Nov 2006
Posts: 24
![]() | http://cgi.ebay.com/Agar-Agar-NATURA...QQcmdZViewItem This is the supplier I used. The whole seaweed is no longer available (I bought it at closeout prices) but they do have the 1lb powdered stuff. The sellers name is Marilyn. If you want the whole seaweed maybe you could ask her to stock some more. I tried to get the name of her supplier with no luck. Another option (if you have a saltwater fish tank) is to buy live Gracillaria and grow your own. You could check your local pet store for some. |
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| | #10 (permalink) |
| Happy and Thankful Join Date: Dec 2005
Posts: 1,738
![]() ![]() ![]() ![]() | Through pet stores and suppliers you would be looking at big buck$. Growing your own is a nifty idea for those with the capacity for a refugium. Especially if they also got some grow lamps.
__________________ Just pretend there is a deep or witty comment here and move along. |
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| | #13 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,053
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Damn, dude, excellent thread. Great pics. I totally got it with almost no words. Very cool! I would like some details on the "de-watering" though. Basically just boiling off the water? Regardless: archive material to agar
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #14 (permalink) |
| Mycophiliac Join Date: Nov 2006
Posts: 24
![]() |
This experiment is turning out better than I had hoped. Three gallons were boiled down to just one. This was cooled down to room temperature and then frozen solid outside for a couple days. Placed on a wire screen at room temperature, allowing water and impurities to wash away. ![]() ![]() After it dries into a cake I'll pop it in a vacuum chamber to remove all the moisture. Last edited by Beast; 03-13-08 at 01:59. |
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