contam on my agar?

bosshogg · 01-30-09, 10:25

wondering if i have a contam on my plate here. it started off as a little white puff along the edge so i thought it was just some stray myc. today i looked at it and it was a lot bigger with a greyish knot in the center. something just doesn't look right to me. any help is much appreciated..

Lazlo · 01-30-09, 10:44

Looks like mold near the edge and a lot of bacteria in the center. Try to isolate a small shaving of mycelium to a new plate quick.

vinz · 01-30-09, 10:45

is the grayish not exactly the center of that growth?
because it seems to have some good myc growing there..
the middle part looks like its contaminated with wet spot bacteria
you can try and isolate that myc growth in a new clean dish
or a dish with h202 agar to clean it up

edit:lazlo's quick im w him

fungusfarmer · 01-30-09, 10:45

Tough question.... I'd say if it's questionable, toss it.

duaut · 01-30-09, 11:05

If you have the patience, chase it. Meaning snag a tiny bit and put it in a new plate, grow it out and keep doing this till you have a clean plate. This is how agar work is done.
I'd let it grow some more and snag a rhizo when it starts.
You'll be learning a valuable skill that is needed when working with dirty wild prints.
Never toss anything, it sooo easy to chase.

bosshogg · 01-30-09, 11:18

thanks for the help guys i'm gonna make some new plates up today and see what i can save. this one was from a clone that i had taken. i think i'll run a multispore along side of the new one i make and see the difference. on a side note the clones i dropped in grain are looking very promising . thanks again

Lazlo · 01-30-09, 11:20

Quote:

Originally Posted by fungusfarmer

Tough question.... I'd say if it's questionable, toss it.

No, don't toss it. It appears that there's mushroom mycelium growing with the mold near the edge of the plate. You'll have to shave a small amount of growth from the edge furthest away from the mold. If you delay, the mold can start feeding and spreading on the mushroom mycelium and will be a nightmare to transfer away from.

coorsmikey · 01-30-09, 23:16

Yeah! what Lazlo said! I got a question though....The stuff by the edge,Does that dark spot appear to be mold? Or is that a piece of clone material that has just turned blue from oxidation?

highflyer · 01-30-09, 23:23

To me it looks like a peice of tissue that is on the edge of the plate. It also looks like that is the only place on the place that has useable mycelium. Id transfer from that corner growth if that is a peice of tissue. The middle has too much bacteria.

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01-30-09, 10:44	#2 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	Looks like mold near the edge and a lot of bacteria in the center. Try to isolate a small shaving of mycelium to a new plate quick. __________________ How can you have any pudding, if you don't eat your MEAT?

01-30-09, 10:45	#3 (permalink)
vinz magician Join Date: Nov 2007 Posts: 1,195	is the grayish not exactly the center of that growth? because it seems to have some good myc growing there.. the middle part looks like its contaminated with wet spot bacteria you can try and isolate that myc growth in a new clean dish or a dish with h202 agar to clean it up edit:lazlo's quick im w him __________________ i know magic

01-30-09, 11:05	#5 (permalink)
duaut Octopus Sun Join Date: Sep 2008 Posts: 285	If you have the patience, chase it. Meaning snag a tiny bit and put it in a new plate, grow it out and keep doing this till you have a clean plate. This is how agar work is done. I'd let it grow some more and snag a rhizo when it starts. You'll be learning a valuable skill that is needed when working with dirty wild prints. Never toss anything, it sooo easy to chase. __________________ You see I have a millions of beautiful fractaline snake things in my head atm

01-30-09, 11:18	#6 (permalink)
bosshogg joe schmoe super tripper Join Date: Dec 2008 Posts: 616	thanks for the help guys i'm gonna make some new plates up today and see what i can save. this one was from a clone that i had taken. i think i'll run a multispore along side of the new one i make and see the difference. on a side note the clones i dropped in grain are looking very promising . thanks again __________________ 'joe schmoe super tripper might think he can eat an 8 of those like he could any other mushy and off to the loony bin,or in the corner crying for their mommy-IL

01-30-09, 10:45	#4 (permalink)
fungusfarmer Mycophage Join Date: Jan 2008 Posts: 103	Tough question.... I'd say if it's questionable, toss it.

01-30-09, 23:16	#8 (permalink)
coorsmikey mycotrophic Join Date: Dec 2007 Posts: 281	Yeah! what Lazlo said! I got a question though....The stuff by the edge,Does that dark spot appear to be mold? Or is that a piece of clone material that has just turned blue from oxidation?

01-30-09, 23:23	#9 (permalink)
highflyer Mycotopiate Join Date: Apr 1971 Posts: 415	To me it looks like a peice of tissue that is on the edge of the plate. It also looks like that is the only place on the place that has useable mycelium. Id transfer from that corner growth if that is a peice of tissue. The middle has too much bacteria. __________________ "A probable impossibility is preferable to an improbable possibility." - Aristotle

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