How can I transfer from agar to LC?

bassplar99 · 03-21-06, 19:21

Well, the title says it all. I am thinking about adding distilled water to the top of the mycellium in the agar, swirling a bit, suck it up and inject. My concern is this, is distilled water considered sterile from the bottle? I could boil it but I would like to avoid waiting the long cooling period. Thanks.

~Hippie3 · 03-21-06, 20:17

i'd boil it.

03-21-06, 22:42

I'd sterilize some water in a jar with some broken glass pieces, then put the agar in and shake it shake shake it shake it like a polaroid picture, you know what to do.....sorry.

Then suck it up into some syringes.

Like Hippie suggests, boil or sterilize the water first.

fucgubarn · 03-21-06, 23:11

have done it exactly like this:

"I'd sterilize some water in a jar with some broken glass pieces, then put the agar in and shake it shake shake it shake it like a polaroid picture, you know what to do.....sorry.

Then suck it up into some syringes."

bassplar99 · 03-21-06, 23:34

Awesome hints, I will definately try this way.

03-22-06, 09:04

Use a glove box or flow hood when you transfer the agar wedge to your lc jar.
If you do it quick enough you may get by without a gb or fh but I would not chance it.

bassplar99 · 03-22-06, 18:59

For tasks like this I have been trying out this simple setup. I lysol around a rubbermaid inside and out and then run the peele ionizer in front for a few minutes then work in the rubbermaid with the lid off (on its side). So far so good, I am working on construciting a glove box but want to make a foldable design.

~Hippie3 · 03-22-06, 19:20

cardboard boxes fold up.

siam_jim · 03-23-06, 05:27

i uaually just scrape off the mycelium without taking agar then put it in the sterlize water..all in a glovebox.
peace
siam

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03-21-06, 19:21	#1 (permalink)
bassplar99 Mycophage Join Date: Jan 2006 Posts: 138	How can I transfer from agar to LC? Well, the title says it all. I am thinking about adding distilled water to the top of the mycellium in the agar, swirling a bit, suck it up and inject. My concern is this, is distilled water considered sterile from the bottle? I could boil it but I would like to avoid waiting the long cooling period. Thanks.

03-21-06, 23:11	#4 (permalink)
fucgubarn Mycotopiate Join Date: Jan 1971 Posts: 654	have done it exactly like this: "I'd sterilize some water in a jar with some broken glass pieces, then put the agar in and shake it shake shake it shake it like a polaroid picture, you know what to do.....sorry. Then suck it up into some syringes." __________________ don't worry, be stupid!

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03-21-06, 20:17	#2 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,677	i'd boil it.

03-21-06, 22:42	#3 (permalink)
~freakachino Guest Posts: n/a	I'd sterilize some water in a jar with some broken glass pieces, then put the agar in and shake it shake shake it shake it like a polaroid picture, you know what to do.....sorry. Then suck it up into some syringes. Like Hippie suggests, boil or sterilize the water first.

03-21-06, 23:34	#5 (permalink)
bassplar99 Mycophage Join Date: Jan 2006 Posts: 138	Awesome hints, I will definately try this way.

03-22-06, 09:04	#6 (permalink)
~dial8 Guest Posts: n/a	Use a glove box or flow hood when you transfer the agar wedge to your lc jar. If you do it quick enough you may get by without a gb or fh but I would not chance it.

03-22-06, 18:59	#7 (permalink)
bassplar99 Mycophage Join Date: Jan 2006 Posts: 138	For tasks like this I have been trying out this simple setup. I lysol around a rubbermaid inside and out and then run the peele ionizer in front for a few minutes then work in the rubbermaid with the lid off (on its side). So far so good, I am working on construciting a glove box but want to make a foldable design.

03-22-06, 19:20	#8 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,677	cardboard boxes fold up.

03-23-06, 05:27	#9 (permalink)
siam_jim Mycotopia Mod Join Date: Oct 2005 Posts: 1,854	i uaually just scrape off the mycelium without taking agar then put it in the sterlize water..all in a glovebox. peace siam