same ol' same ol': dried tissue mycellial syringes

[fahtster]

hey yall... I haven't posted anything in awhile and i'm bored so i thought i'd show ya what i'm up to.. nothing really differnet here..
this is the same isolate ive had for over a year.. but everytime i use it (which is about once a month) it's only on it's second 'round' of use.. what i did was make 24 'faht jars' (i don't what else to call them) with about 1/2 inch of corn in the bottom and knocked those up with the isolate..
then, just before they colonize the corn, I remove all the tape from the tyvek (except for the tape covering the innoc. hole) that would normally keep the jars from drying out... this allows them to dry out.
i do this right before the jars are colonized so that the mycelium doesn't go into fruiting stage. this helps keep sessenence (sp?) at bay. mycelium can be reanimated so these dried out 1/2 pints last forever..
drying them out also helps keep bacteria down i believe. it's also easier to 'harvest' more myc. when they are dry because the myc. becomes brittle and breaks into very very small particles which is nice. I harvest the myc. by injecting plain sterilized water thru the tyvek via syringe... then let it sit for a few minutes and shake it up. then suck the myc. laden water back up. then shoot that into a covered sterilized 1/2 pint filled with water that i suck up with how many ever syringes i need for the task at hand. the last two pictures are of what i was just talking about.. the dried up myc. corn ready for harvest at will. then when i use the faht jar.. i just replace it with another one... so when it gets used again in another year it'll only be on it's third 'round'.
also, a dry ounce was already picked from this bin prior to taking the pictures..
yummy yummy

fahtster

[~Hippie3]

dayum

mighty fine
as always

[~Hippie3]

wait a sec-
i'm missing something-
that's not a thin layer of popcorn fruiting,
right ?
you're using the pcorn to spawn with yes ?
or ????

[fahtster]

i re edited it now so it should read better... i use that dried up corn to harvest the myc. from.. so yes, i use it as spawn in a way thanks for the kind words.

fahtster

[~Hippie3]

alrighty, that's what i thought
but re-reading and looking closer
only made me confused

so the cakes are pf style ?
nice isolate i'd say.
from which 'strain' was the isolate derived ?

[fahtster]

hehehe... basically i knock up some corn in 1/2 pints, let them dry out, and harvest the mycelium so that i can make syringes with that myc. to knock up pf jars. i don't fruit out the corn. I use the corn as kind of a 'crumbling agar' to grow more myc. that i can knock up pf jars with or whatever the growing medium may be. these are double stacked 1/2 pints.. so there are 24 1/2 pints in that bin.. it's good ole texas.

fahtster

[~Hippie3]

how fast does the dried tissue re-animate
and how fast, once revived, does it grow,
i.e. how does it compare to either live cloned tissue
or even multi-spore
time-wise ?

[fahtster]

it takes about two days to see visable growth once re-animated... I think it's a little bit slower than live tissue but not much definately beats out spore innoc. live clone tissue is pretty instant i would think... once it hits the substrate it's on i'm guessin.. but couple days isn't all that bad.

fahtster

[fahtster]

oh yeah, for your other question... the mycelium grows normal once revived.

[~Hippie3]

how long is the dried tissue viable,
able to re-animate ?
what's the longest you're heard of between drying and use ?

[fahtster]

well.. I'm going on over a year for mine... haven't really heard a whole lot.. someone mentioned that mushroom tissue could be re animated... i think it was stephan peele.. it was awhile ago.. I was already using the 'faht jars' but i was 'harvesting' the myc. when it was still growing.. then i got the idea to dry them out and it actually worked lol.. then everything came from that.. i.e. making 24 'faht jars' to prolong the substrain use without damaging the strain from constant cloning and getting mutations... eventually the strain will mutate but it will take quite awhile for that to happen i think.. years +

fahtster

[fahtster]

and 24 was kind of excessive... i probably would have been happy with 12 faht jars... oh well

faht

[~Hippie3]

do you store the dried tissue
frozen or room temp ?

[fahtster]

i store them at room temp.. but i was thinking that the fridge would be better.. don't know about the freezer tho.. there still is some water in the corn and myc. i would think.. so the frozen water crystals may ruin the myc. cells. I guess i could try it once. i haven't been the greatest at storing my jars.. didn't really put all that much thought into it. I just keep em in my closet. lol i haven't seen any ill effects tho, so maybe thats why i haven't paid all that much attention.. but i would definately recommend the fridge and wrap jar in tin foil to keep out light. I've been thinking about doing a new strain or finding the mazzies again.. really liked that strain.. so all the jars i made probably aren't going to get used. tis life.

fahtster

[Texas_Bob]

Nice lookin thread you have goning here. Are those pics of an Isolate that you produced through your fahhty isolation tek? Or an isolate worked from agar? I am, currently useing your tek with some wild Florida Cope strain and I hope they look as good as your Tex isolate. Nice work

[fahtster]

thanks bob, yep those are an isolate using my tek. glad to hear you're using it also. makes me feel all tingly inside. lol

fahtster

[sinthetic]

I tried the fahtty iso tek. A little bit of corn was put into the bottom of the jar, with a couple of screws. Once fully colonized i squirt water in a shake it about, then draw the water back out.
This usually makes for nice myc syringes. Somone suggested to me sucking a bit of karo out of a sealed jar (with airport) into the syringe as well.

Great info and nice isolation.

[Texas_Bob]

Some others here are using your tek Too!! Thanks for a great isolation tek that others without agar/flowhood can enjoy nice full and beutiful flushes. http://www.forums.mycotopia.net/showthread.php?t=5693

[Texas_Bob]

The airport method for injecting fresh filtered air periodically. Thanks Hippie http://www.forums.mycotopia.net/showthread.php?t=5245

[fahtster]

nice and even if you don't use that tek for isolation.. it's still good for making mycelium syringes eh.

fahtster

Imagine that. Just a year ago, nearly everyone thought it's impossible to revive dry tissue. Nice stuff.
RR

[~Hippie3]

actually it's been known several years now,
just not widely practiced.
i remember seeing a very similar method to faht's corn-in-jar
way back in drooldonkey days, old doc charlie reported dried kernels
re-animating many months after drying out,
that's gotta be ~7 years ago,
and i'm sure if you check with other old timers
like peele you'll find it's even older than that.

[~Hippie3]

archive material

[blackout]

there was a guy doing it about a month ago. posted in advanced at the shoomery. It may not have been fully dried.
A professional grower (of medicinals) came on and talked about sticking wooden sticks in agar plates. Said the wood would colonise and last years after the agar was spent/dead. I am think cubensis will colonise sticks, I would use cocktail sausage sticks or coffee stirrers.

[~Hippie3]

I am think cubensis will colonise sticks,

it will indeed.
cardboard might be nice too
one could fold it shut for long term storage
kinda like a print

i tryed it a while back hipp..
dryed cardboard that is
tryed a peroxide dip and into a karo jar
grew nothing but mold
now i'm sure if somebody was using agar
it would be a great way to preserve a strain
matter of fact.. i'll try it again now that i got my agar stuff running

fhat great stuff man
i used your tek several times in the past with great success now i wish i had saved them pesa jars.. as i had one that really kicked ass
lost

Very nice and interesting work! I have used your isolation tek for a while now and it always works great! Now to know I can dry these and store them to re-animate is an added bonus! Great great great!!!!!

[fahtster]

hey yall,
just some update pix on the bin.. I love these tex so much cuz they dry in a day during the winter and fall months when the rh is as low as it gets. heres a few pix of the final outcome of the entire bin..
the dry weight was 129 grams.. so thats about 5.4 dry grams per cake on average. not too bad.. i've see/done better and worse.. these cakes were kinda of flop tho.
I was late birthing them due to the fact that i put too much water into the cakes when i was mixing them... I was trying to up the water content so that it was like as if the cakes were dunked prior to the first flush. but instead they clumped up and big air pockets formed..
this slowed down colonization. a few mushrooms actually grew from inside the cakes where the air pockets were and pushed the side off the cake. I didn't do the inner res. tek with these either like i usually do..
I now realize that besides providing the cakes with an inner res.. it also reduces those air bubbles as i push the sharpie into the cake and out to the sides.
plus this is only the second batch of cakes I've done since a long hiatus that i took from them when i worked just with corn. I think the next batch is going to be a lot fuller. cake mushies are soooo much easier to clean than corn mushies hehe. I'm glad you all like the tek. makes me happy.
fahtster

[~Hippie3]

about 5.4 dry grams per cake on average

that's a pretty decent average for one flush.