Cloning Shitake

kocos · 07-16-07, 02:54

So after dumping the drystone cloning, i was in need of trying something else. Iam sure the techs are here but i just cant seem to find them. Upon visiting the market on saturday, i found small baskets of fresh shitake for sale, immediatly i thought about cloning and growing my own, so i bought a small basket. I tried making some prints but it seems that these for some reason dont sporulate so i didnt get any prints, ended up making some fried rice with shitake and shrimp

Ok so i saved two of the most picture perfect fruits i had in the batch and iam going to clone these. My questions are the following:

1: since shitake is a wood lover, would i have to make "wood water" agar, or would regular potatoe, carrot agar do? Furthermore does honey work in agar? I dont have maltextract...

2: Substrate, if i manage to get the agar to work, what substrate can i use for the first stage? Iam assuming the regular BRF tek is out of the question for this one. Iam guessing oak sawdust, and something else in adition would be fine?

I think for the first part i would need to culture some live mycelium, so lets just start with that and disregard the substrate. Agar? LC? Cardboard? What are the recomendations, where should i start? Any advise is greatly apretiated.

crazy1 · 07-16-07, 06:55

I cloned some Shiitake not too long ago. I used MEYA and had good results. I'm sure if you use the honey it'll be fine. But I'd also add a bit of sawdust to your agar. This will cause the mycelium to produce the enzymes needed to digest wood. It will remember this and you'll have quicker leap off time when you move to the bulk.

kocos · 07-16-07, 07:26

So do u suggest i make a regular honey based agar, but instead of water, i boil lets say some oak and uses that water to make the agar?

crazy1 · 07-16-07, 07:34

No need to do all that. Just toss a little sawdust into the mix. That's really all you need.

Lazlo · 07-16-07, 10:42

A standard MEA or PDA works fine.

kocos · 07-17-07, 02:54

Thx guys, i just need some parafilm now, i dont dare start without it. And it lookslike i found a shop yayyy

Lazlo · 07-17-07, 09:10

1" 3M masking tape works great.

crazy1 · 07-17-07, 09:13

never thought of that Lazlo, great idea

Lazlo · 07-17-07, 09:16

Don't use the cheap painter's stuff. If you do, the plates need to be wrapped twice with it. The 3M stuff works fantastic with the plates only wrapped once.

crazy1 · 07-17-07, 09:17

cool, thanx

joystik · 07-17-07, 23:04

how long does a shiitake take to recover and start growing after transferring onto agar?

kocos · 07-18-07, 04:35

We should find out soon enought, i had a call in the morning, they received on last role of polyfill. Now my only problem is that i dont have malt extract. Ill have to brew that potatoe and carrot agar, with a bit of honey. Someone stop me if this is a bad idea. Ill also be ading some sawdust to the mixture which i also have to collect forgot yesterday. But in anycase it looks like ill be making like 3 shitake plates and also cloning that monster ksss, on 3 other plates. So plan is to cook up 500 mils of agar, wait till its cool but still fluid, ad 5 cc-s of 3%peroxide. Mix the shit out of it then pour out into plates, all done in a GB ofcourse. Gonna by a huge bottle of isoalcohol to spray GB with and disinfect hands. I really hoope i can get my shit together and finally get some plates with clean growth, no bacteria patches or mold. Fingers crossed. Ill be making a nice pictorial of the process. Till then peace and luv.

spyker · 07-18-07, 11:18

Why dont U just clone to a disk of cardboard, U know the brown thick corrogated one, its selective and works like a charm. Cut the disk to fit the bottom of your plates or jars, wet, pour off excess moisture, then PC as usual...

joystik · 07-18-07, 13:38

interesting, didnt occur to me. Ill do just that.

About my question above, does anyone know?

JoY

Lazlo · 07-18-07, 14:07

Shiitake have average speed.

kocos · 07-19-07, 03:30

Ok i didnt have time to collect wood yet, so yesterday i just cloned the ksss, and the shitake ill be doing today after ive found some wood, might actually have to do it tomorow. In anycase i have a few pics of the preperation, and the plates them selfes. I was alone last night so no pics of the process, but ill update once i get to the shitake. Should be monday i guess. So pics, first the taters soak, then agar is made, while thats cooking the things needed are cleaned and placed into the "lab". agar and dishes are put into GB, GB is taped, sprayed with iso alcohol inside, and allowed to cool. Once it started to thicken and cool, 3 cc-s of 3% peroxide was added and mixed in well. Agar was poured out and allowed to harden. Last pics are of the plates, not sure why they seem to have so much condensation its not that bad in real life, and it was just the top dish which had some condensation. Last pic is of a KSSS run, at day 2

07-19-07, 16:22

great work kocos!

kocos · 07-23-07, 04:42

Ill have pics tomorow, but so far it seems out of the 4 dishes one will be superb. The other 3 are strange, it almost seems as if it was myc growing out from the tissues but it isnt, the agar is just whiteish around the tissue and its spreading at a great pace. No idea what it can be

Ill bring in pics tomorow and show u guys

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07-16-07, 02:54	#1 (permalink)
kocos Mycotopiate Join Date: Jan 2007 Posts: 851	Cloning Shitake So after dumping the drystone cloning, i was in need of trying something else. Iam sure the techs are here but i just cant seem to find them. Upon visiting the market on saturday, i found small baskets of fresh shitake for sale, immediatly i thought about cloning and growing my own, so i bought a small basket. I tried making some prints but it seems that these for some reason dont sporulate so i didnt get any prints, ended up making some fried rice with shitake and shrimp Ok so i saved two of the most picture perfect fruits i had in the batch and iam going to clone these. My questions are the following: 1: since shitake is a wood lover, would i have to make "wood water" agar, or would regular potatoe, carrot agar do? Furthermore does honey work in agar? I dont have maltextract... 2: Substrate, if i manage to get the agar to work, what substrate can i use for the first stage? Iam assuming the regular BRF tek is out of the question for this one. Iam guessing oak sawdust, and something else in adition would be fine? I think for the first part i would need to culture some live mycelium, so lets just start with that and disregard the substrate. Agar? LC? Cardboard? What are the recomendations, where should i start? Any advise is greatly apretiated.

07-16-07, 06:55	#2 (permalink)
crazy1 VIP Member Join Date: Apr 2007 Posts: 868	I cloned some Shiitake not too long ago. I used MEYA and had good results. I'm sure if you use the honey it'll be fine. But I'd also add a bit of sawdust to your agar. This will cause the mycelium to produce the enzymes needed to digest wood. It will remember this and you'll have quicker leap off time when you move to the bulk. __________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience

07-16-07, 07:34	#4 (permalink)
crazy1 VIP Member Join Date: Apr 2007 Posts: 868	No need to do all that. Just toss a little sawdust into the mix. That's really all you need. __________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience

07-16-07, 10:42	#5 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,052	A standard MEA or PDA works fine. __________________ How can you have any pudding, if you don't eat your MEAT?

07-16-07, 07:26	#3 (permalink)
kocos Mycotopiate Join Date: Jan 2007 Posts: 851	So do u suggest i make a regular honey based agar, but instead of water, i boil lets say some oak and uses that water to make the agar?

07-17-07, 02:54	#6 (permalink)
kocos Mycotopiate Join Date: Jan 2007 Posts: 851	Thx guys, i just need some parafilm now, i dont dare start without it. And it lookslike i found a shop yayyy

07-17-07, 09:10	#7 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,052	1" 3M masking tape works great. __________________ How can you have any pudding, if you don't eat your MEAT?

07-17-07, 09:13	#8 (permalink)
crazy1 VIP Member Join Date: Apr 2007 Posts: 868	never thought of that Lazlo, great idea __________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience

07-17-07, 09:16	#9 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,052	Don't use the cheap painter's stuff. If you do, the plates need to be wrapped twice with it. The 3M stuff works fantastic with the plates only wrapped once. __________________ How can you have any pudding, if you don't eat your MEAT?

07-17-07, 09:17	#10 (permalink)
crazy1 VIP Member Join Date: Apr 2007 Posts: 868	cool, thanx __________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience

07-17-07, 23:04	#11 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 297	how long does a shiitake take to recover and start growing after transferring onto agar?

07-18-07, 04:35	#12 (permalink)
kocos Mycotopiate Join Date: Jan 2007 Posts: 851	We should find out soon enought, i had a call in the morning, they received on last role of polyfill. Now my only problem is that i dont have malt extract. Ill have to brew that potatoe and carrot agar, with a bit of honey. Someone stop me if this is a bad idea. Ill also be ading some sawdust to the mixture which i also have to collect forgot yesterday. But in anycase it looks like ill be making like 3 shitake plates and also cloning that monster ksss, on 3 other plates. So plan is to cook up 500 mils of agar, wait till its cool but still fluid, ad 5 cc-s of 3%peroxide. Mix the shit out of it then pour out into plates, all done in a GB ofcourse. Gonna by a huge bottle of isoalcohol to spray GB with and disinfect hands. I really hoope i can get my shit together and finally get some plates with clean growth, no bacteria patches or mold. Fingers crossed. Ill be making a nice pictorial of the process. Till then peace and luv.

07-18-07, 11:18	#13 (permalink)
spyker Mycotopiate Join Date: Jun 1972 Posts: 285	Why dont U just clone to a disk of cardboard, U know the brown thick corrogated one, its selective and works like a charm. Cut the disk to fit the bottom of your plates or jars, wet, pour off excess moisture, then PC as usual...

07-18-07, 13:38	#14 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 297	interesting, didnt occur to me. Ill do just that. About my question above, does anyone know? JoY

07-19-07, 16:22	#17 (permalink)
~greysRDbest Guest Posts: n/a	great work kocos!

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07-18-07, 14:07	#15 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,052	Shiitake have average speed. __________________ How can you have any pudding, if you don't eat your MEAT?

07-23-07, 04:42	#18 (permalink)
kocos Mycotopiate Join Date: Jan 2007 Posts: 851	Ill have pics tomorow, but so far it seems out of the 4 dishes one will be superb. The other 3 are strange, it almost seems as if it was myc growing out from the tissues but it isnt, the agar is just whiteish around the tissue and its spreading at a great pace. No idea what it can be Ill bring in pics tomorow and show u guys

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