Is it possible to clone reishi in agar?

joystik · 07-20-07, 15:17

Hi, i just found a stump at the local golf course that has some very reishi looking growth. Ill post the pics later, when i get the camera. I have been wanting to get this species for a long time now and i wonder if it can be cloned in agar.
Does it have to be a special agar recipe or will the standard MEA work just fine?
JoY.

joystik · 07-20-07, 20:01

Just adding some pics. Im not 100% sure this is reishi (Ganoderma Lucidum), but it looks really similar. Hope someone can confirm.

07-20-07, 22:01

looks like reishi to me!

from what i know, it can be cloned from outdoor tissue.. have no experience myself tho..
i also know that reishis are freakin' hard.. good luck cutting that up

no special agar needed.. any would do

~Hippie3 · 07-22-07, 19:10

agreed, looks like reishi
nice
don't recall any agar/reishi threads here b4
so this will be newsworthy.
plz post any updates as you progress,
thx

07-22-07, 19:18

good lookin reishi...young one and it looks like it may have been antler form which is highly prized. good luck with it !!

joystik · 07-23-07, 12:24

What part of the mushroom should i use for cloning? I never done this species before so i wouldnt know.

Thanx.

Lazlo · 07-23-07, 12:32

The outer edge of the mushroom. It's very hard to get in there even. The mushroom wants to split into small sections. The best you can do is; split the outer edge in a sterile environment and use a super sharp scalpel or a tweezer to snag out a strand of the tissue. It's not that hard really, but be prepared to transfer the growth to a new plate.

joystik · 07-24-07, 10:50

Ok. I tried that and after 48 hours i see no signs of growth nor contamination. However i i will keep this updated if any change. If this doesnt go well ill try again. I know where to find more.

Joy.

joystik · 07-25-07, 09:03

Ok. My last post was 24 hours ago. I cant really believe my eyes, this thing exploded in 24 hours. I still cant believe what i see. but i confirmed with the other sample, which is not as big, but its growing too, a bit slower tho.

You can see them in the pics. these are the two samples i picked from the mushroom above. The first one showed signs of growth last night, before i went to bed. The second one just now. Sorry for the crappy pics. When my GF brings me the camera ill take proper pics.

Any comments welcome.

Joy.

kocos · 07-25-07, 09:06

thats wicked man, congrats

~Hippie3 · 07-25-07, 09:14

When my GF brings me the camera ill take proper pics.

Any comments welcome.

ok,
be sure to take some 'improper' shots, too, for the bnl .

Lazlo · 07-25-07, 09:15

Nice job.

Now you'll certainly want to transfer the leading edge of the mycelium to a new dish and don't be afraid to do a few more transfers as well. There's nothing worse than thinking you're golden with a clean culture and then after a few days a bread mold starts growing all over your mushroom's mycelium. By that point the project has been set back a lot and transfers could be more than 10.

I know it's a pain in the ass, but trust me on this one.

joystik · 07-25-07, 10:03

Quote:

Originally Posted by Lazlo

Now you'll certainly want to transfer the leading edge of the mycelium to a new dish and don't be afraid to do a few more transfers as well.

Ill do this this afternoon. Thanks.

Joy.

joystik · 07-26-07, 09:42

I just wanted to up some better photos of the culture bottles. This was last night.

JoY.

~Hippie3 · 07-26-07, 17:45

are you laying those down and pouring agar in them ?

kocos · 07-27-07, 02:59

Wow that right one seems to be nice and healthy. Do you have any wood in that agar?

joystik · 07-27-07, 07:54

Hip: I pour and sterilize, then lay down.

Kocos, No, this agar doesnt have any wood.

I was gonna do wood agar but forgot the wood, lol.

bb4 · 07-27-07, 08:09

i like the idea for a poor-man's petri there, so many extra bottles / jars lying around, wish i'd of thought of that before i invested in my petris,

keep up the good work mang,

-bb4

kocos · 07-27-07, 08:49

Seems to be doing very well non the less. Are you going to transfer to "woody agar"?

joystik · 07-27-07, 09:46

i think im going from here to sawdust. an then bulk.(more sawdust)

Joy.

~Hippie3 · 09-23-07, 20:45

did this progress ?

joystik · 09-23-07, 20:56

Sadly, all samples contaminated. I had to throw away everything. I will try again as soon as i get a new specimen, since the last one i used it in an infusion for my GF, which by the way worked out very well.

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07-20-07, 15:17	#1 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	Is it possible to clone reishi in agar? Hi, i just found a stump at the local golf course that has some very reishi looking growth. Ill post the pics later, when i get the camera. I have been wanting to get this species for a long time now and i wonder if it can be cloned in agar. Does it have to be a special agar recipe or will the standard MEA work just fine? JoY.

07-23-07, 12:32	#7 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,692	The outer edge of the mushroom. It's very hard to get in there even. The mushroom wants to split into small sections. The best you can do is; split the outer edge in a sterile environment and use a super sharp scalpel or a tweezer to snag out a strand of the tissue. It's not that hard really, but be prepared to transfer the growth to a new plate. __________________ How can you have any pudding, if you don't eat your MEAT?

07-25-07, 09:15	#12 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,692	Nice job. Now you'll certainly want to transfer the leading edge of the mycelium to a new dish and don't be afraid to do a few more transfers as well. There's nothing worse than thinking you're golden with a clean culture and then after a few days a bread mold starts growing all over your mushroom's mycelium. By that point the project has been set back a lot and transfers could be more than 10. I know it's a pain in the ass, but trust me on this one. __________________ How can you have any pudding, if you don't eat your MEAT?

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07-20-07, 22:01	#3 (permalink)
~vinz Guest Posts: n/a	looks like reishi to me! from what i know, it can be cloned from outdoor tissue.. have no experience myself tho.. i also know that reishis are freakin' hard.. good luck cutting that up no special agar needed.. any would do

07-22-07, 19:10	#4 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	agreed, looks like reishi nice don't recall any agar/reishi threads here b4 so this will be newsworthy. plz post any updates as you progress, thx

07-22-07, 19:18	#5 (permalink)
~greysRDbest Guest Posts: n/a	good lookin reishi...young one and it looks like it may have been antler form which is highly prized. good luck with it !!

07-23-07, 12:24	#6 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	What part of the mushroom should i use for cloning? I never done this species before so i wouldnt know. Thanx.

07-24-07, 10:50	#8 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	Ok. I tried that and after 48 hours i see no signs of growth nor contamination. However i i will keep this updated if any change. If this doesnt go well ill try again. I know where to find more. Joy.

07-25-07, 09:06	#10 (permalink)
kocos The PsYhead Join Date: Jan 2007 Posts: 1,375	thats wicked man, congrats

07-25-07, 09:14	#11 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	When my GF brings me the camera ill take proper pics. Any comments welcome. ok, be sure to take some 'improper' shots, too, for the bnl .

07-26-07, 17:45	#15 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	are you laying those down and pouring agar in them ?

07-27-07, 02:59	#16 (permalink)
kocos The PsYhead Join Date: Jan 2007 Posts: 1,375	Wow that right one seems to be nice and healthy. Do you have any wood in that agar?

07-27-07, 07:54	#17 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	Hip: I pour and sterilize, then lay down. Kocos, No, this agar doesnt have any wood. I was gonna do wood agar but forgot the wood, lol.

07-27-07, 08:09	#18 (permalink)
bb4 Lurker Extraordinaire Join Date: Nov 2006 Posts: 238	i like the idea for a poor-man's petri there, so many extra bottles / jars lying around, wish i'd of thought of that before i invested in my petris, keep up the good work mang, -bb4

07-27-07, 08:49	#19 (permalink)
kocos The PsYhead Join Date: Jan 2007 Posts: 1,375	Seems to be doing very well non the less. Are you going to transfer to "woody agar"?

07-27-07, 09:46	#20 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	i think im going from here to sawdust. an then bulk.(more sawdust) Joy.

09-23-07, 20:45	#21 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	did this progress ?

09-23-07, 20:56	#22 (permalink)
joystik Mycotopiate Join Date: Nov 2006 Posts: 481	Sadly, all samples contaminated. I had to throw away everything. I will try again as soon as i get a new specimen, since the last one i used it in an infusion for my GF, which by the way worked out very well.

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