psilocybe mexicana sclerotia-clone

[kocos]

So this is next project, iam getting some unknown dry sceletoria in the coming days which iam wanting to clone. Now i think the best would be if i would do this on agar, iam also getting some malt extract so shouldnt be a prob to make some agar plates or alternitavly some LC-s. Now since ive never cloned a sceletoria or any dry matter i would like some pointers on how to go about doing this. My initial plan:
1,Cook agar, pour out into plates, let cool, all done in GB.
2, Once cool, cut up sceletoria, remove iner tissue, dip into 3% peroxide, and transfer to plate. Seal with special tape i forgot the name of.
3, Incubate, transfer incase of contams.

So how does this sound? What are the chances? Any input is apretiated

peace pot and love

i don't know about dry.. but i have cloned fresh stones before
i broke the stone in half, don't cut it.. then i dug a chunk of it out of the center where its still sterile.. put maybe 3 or 4 peices per plate.
then just transfer when you see some good clean growth.
did that about 2 years ago.. and threw g2g i still got jars of stones going. actually i need to eat some of them, lol

[PsilocibeJuggalo]

Cloning is getting living tissue to replicate. If it is dry it is dead.

(bad information)

[Workman]

Dry sclerotia is rock hard. It will be difficult to cut. Assuming its alive, surface sterilization of a whole sclerotium in peroxide dropped directly on agar should work. Its going to need to rehydrate on the agar before it grows but that shouldn't be a problem. If you insist on using inner tissue, surface treat in H2O2 and then rehydrate in sterile distilled water. It will then be soft enough to get at that inner tissue which should be sterile. An antibiotic agar or the addition of a small amount of salt to the agar will help slow or prevent bacterial growth. Even if you see bacteria soon after a transfer, give it time as the mycelium can often start late and still overgrow the contaminates. Success will be indicated by a white fuzz appearing all over the dark sclerotia before leaping off to the agar.

Alternatively, you could attempt to grow a mushroom directly from the sclerotia by planting the sclerotia in a moist casing.

Good luck!

[kocos]

Thx for the great advise guys. I got the stones yesterday, they really are stones, rock hard. But there is enought to experiment with, so i think one or two will be treated with peroxide rehydrate in sterile water and moved to agar, and one will be treated soaked and taken apart for inner tissue. Ill try with both salt treated and antibiotic agar. Ill get things updated once i have everything set, my bigest prob is petri dishes. Thanx again for the great advise.

Peace

[kocos]

Guys quick question. Is it normal for scletoria to be sour as hell, as if taking a bite from a lemon? At first the taste was normal msuhroom like, then out of no where this intence sour taste flooded the back our our mouths, and not even beer wanted to wash it away completly. Is this normall? oR is it rather spiked with something?

[Pedestrian]

Originally Posted by PsilocibeJuggalo

Cloning is getting living tissue to replicate. If it is dry it is dead.

Funny... People clone dry stuff all the time.

good luck w this kocos!!
hope it turns out well for ya..
thinking you got that sclerotia from somewhere.. it might not be clean so getting the inside is a good idea
also, i suggest h202 agar..
heh

iv read teks before in cloning dry mushroom tissue that they soak it in distilled water for a few days? not sure then dip it in h202 before placing it on agar..

[kocos]

Dunno man, first i wanna find out if its normal that they taste so sour, if its not normal then i wont be cloning this one. I did finally get petri dishes though glass ones too

nice
i use glass dishes and reuse them all the time
if i reuse them, just wash then bleach dip for 10mins at least
and then pour h202 agar
if i reuse it for agar only and not peroxide agar, i wrap it in aluminum foil and PC it
thats a good investment

[Lazlo]

Originally Posted by PsilocibeJuggalo

If it is dry it is dead.

Not true.

Hey kokos, could you do some photography of the process and progress?

[kocos]

Dude as soon as someone tells me if the sour taste is normall ill get to work and post pics of the process. If the sourness is not normall ill have to wait for another specimen or ill just clone something else thats dry. Iam still looking for parafilm, looks like ive found it now, and iam getting malt extract, i have the lab grade agar. Sooo its just this tissue sample causing confusion at the moment, why is it soooo damn sour? Is that normal?

i use gladware cling wrap

[~Hippie3]

Originally Posted by PsilocibeJuggalo

Cloning is getting living tissue to replicate. If it is dry it is dead.

wrong.
plz do not speak up giving advice if you are unaware of the facts.
Mycotopia Web Archive: CloningUsing Dry Tissue

well the stuff i've eaten taste kinda nutty to me
a weird dry mouth kinda after taste, i guess you could call it sour
personaly i just broke it up into pill sized chunks and swallowed with some koolaid
lost

[Workman]

I wouldn't call it sour, more of an acrid aftertaste.

[kocos]

Originally Posted by Workman

I wouldn't call it sour, more of an acrid aftertaste.

Ok well in that case i think iam dumping my sample. Upon ingesting a stone, the taste was normal, fungi like, but when it reached the back of my mouth the sour taste was overwhelming very much like when iam sick and i drink vitaminc cristals disolved in water. Perhaps someone spiked the stones with vitamin c? I might still give it a shot, you never know, but i think ill just clone somthing else thats dry for now and when i get my hands on some "clean" stones ill give this a shot. Thanx for the great input everyone, i will be doing this experiment sooner or later. Patience

[Lazlo]

You may have eaten a grain of grass seed also. They taste terrible and somewhat sour. They'll definitely give you the bitter beer face.

[kocos]

Originally Posted by Lazlo

You may have eaten a grain of grass seed also. They taste terrible and somewhat sour. They'll definitely give you the bitter beer face.

Could have been but it wasnt, it was small little brains we were eating, def not grains, so it must have been soaked in somthing.. oh well, looks like ill be getting a jalisco print soon. Ill make me own stones, dry one and clone that just for the fun of it

[Workman]

The Jalisco strain of P. mexicana has been selected for mushroom production and isn't the best sclerotia producer. Unless you are talking about a wild strain of mexicana from Jalisco.

[blackout]

I tried cloning some dried atlantis a month ago, also some frozen fresh ones. Dipped in H202 and onto a honey & potato based agar. Nothing but green. Will try again though. I also did a old shroom at the time, it did grow but it could have been spores on its stem. I also found some mini shrooms on a discarded plate, now dried I dumped them in LC but see no growth. In fact I have never had growth by transferring tissue to LCs, be it mexA or atlantis sclerotia or shroom tissue.

[blackout]

Well I have old jars of sclerotia cores and one seems to be colonising. Might have success at last.

I made jars of sterile water with self healing silicone injection ports on them. After a grow I would take a big stone, clean the outside with H2O2 and inject a syringe right through it, this takes a core out of the centre, this can then be injected into the jar. No need for a glovebox but no harm either. The cores usually showed a bit of growth, maybe cannibalising themselves, or just stretching out like in a casing with no nutrients.

Anyway I sterilized a small jar of half honey, half water. Then injected some into 3 of these old jars. One looks like it might be showing new growth. To make sure I have just prepared a new larger LC. The current jar has only about 50ml in it. When I transfer to the new jar any new growth will be more apparent.

I am not sure what the strain is though! it could be 1-3 years old by now, and at room temp. It is either mex A, or atlantis from sporeworks. It is also possibly a commercial strain of mexicana from holland, that could well be a sporeworks strain too.

Will keep you posted on progress, doesnt look like contams anyway.

[kocos]

Interesting man, give us some more details if things work out, and some pics would be nice too This project is on hold for now, untill i can get some better tissue to work with.

[OooO]

till now i didnt see a thread about this: i grew some psilocybe mexicana jalisco. after a few flushes i searched the substrate for sclerotia. i did the experiment to clean/rub of one sclerotia with 98% alcohol and trew it in honey/water after a week or so the sclerotia began at room temperature to produce mycelia. this liquid culture works really fine. thought i shoult mention this! got some nice pics-how does it work to show them?

[chimp]

IN order to post pictures see the icon above the text box that looks like a paper clip..click on that and follow the directions.

[OooO]

well here it comes on the first pic you can see right on the bottom the sclerotia and a piece of glas

[OooO]

now:

[chimp]

cool! i have some stones in a jar right now.. il let it grow out more then clone some on agar then to LC or agar to grain
did you try your LC yet?

[OooO]

yes i did-and so far its working fine- i cased one jar till now and now i'm waiting for fruiting

is that psilocybe mexicana strain A?
or psilocybe jalisco?

[OooO]

jalisco strain

[OooO]

well this is the cased psilocybe mexicana-after a few weeks of waiting for pinning i changed the fruiting chamber-humidity was too high- and now there a forming pins and sclerotia right on the top layer:
IMGP0659.JPG

IMGP0664.JPG

cap.JPG
you see this crazy mush-cap - its popping right throu casing layer -without any stem!