My first Caerulipes.. [merged]

[waylitjim]

Congrats Golly!
There's only a handful of people who've cultivated Psilocybe caerulipes. You are now one of them! Your patience and dedication is honorable.

[Foster]

Wow! Looks like they like it pretty damp. Great work! Surprisingly my tray looks pretty good this time. Have you had a chance to test these yet? hehe! foster

[SharkieJones]

Congrats Golly Impressive!

[golly]

Thanx for the kind words...
The fruit take a bit longer than Cubes to mature , proly harvest the first ones tmrw..I'll test for potency Wed or Sat..

Gluk with your grows guys ...patience and fresh air seem to be the key players,,the myc is quite tough and aggressive ,so it doesn't have to be coddled...

[~Hippie3]

archive material

[golly]

Cool..!.This cluster is about 7 days old and i'm trying to print em now..

Also making some clones for a new outdoor patch..

[the_chosen_one]

[Lazlo]

Nice stuff man! Very nice.

Any kind of odd steps to the fruiting process?

[golly]

Not really Laz..The one tub that i put in the freezer is the one that has not fruited yet...I think they just need time to engulf the wood chips..the temps have generally been warming over the last few weeks and proly stimulated
fruiting..

I have 3 fresh prints to give away, to any one who has posted in this thread
so far... [1 each] ...Just briefly tell how u want to grow em...

So the first 3 peeps gets m'

As more become available, i will offer them too...

[CoyoteMesc]

I'd like to say,
I think my post got deleted?
jk
Man, good luck for those that earn the
prints


lol

[Foster]

What a kind offer golly! I hope whomever takes you up on it, and has a fruitful experience. Atm, all stocked up here. However.... hehe.. at a later time....or when you have extras, you may hear from me lol.

I'm Sooo happy to see a succesful attempt at these ! Inspiring for sure!

2 slightly contaminated (wet spot, and a lil lipstick or purple mold) jars of rye, hickory chips, and birch toothpicks were spawned to an outdoor bed today.

The bed was prepped beneath a rhododendron last fall. It contains a mixture of castings, top soil, and hickory wood chips.

Nice rhizo myc being txferred to agar plate tonight.

foster

[mycobri]

that is very nice of you!
have a half print still and it seems pretty clean

let us know how they are!

[joey_megabucks]

awesome work. great job

[llamabox]

Man...how can I be so happy for you and depressed at the same time? I have never recieved fruits for my labors and that is even trying the transfer outdoor myc trick.

But I know now half of my problem was lack of fresh air, so maybe I will try again.

[golly]

U need prints Llama..? they should be pretty clean....I only used free standing caps....I couldn't have done it with out your donation...

[llamabox]

No, but thanks for the offer. I think I have 1-2 laying in the fridge.

[golly]

OK ,then i''ll open this offer to the rest of the Forum - all u need is 200 posts to qualify...
Here's the first tub i made up in November with Flax growing..About 7 pins showing on top + a few down below so far...

[CoyoteMesc]

Hey Golly,
I would like to get one and start it on agar(what recipe would fit these well?) then
spawn it to some ground birch and/or maple.
after that colonizes I would like to do a tek similar to this
http://forums.mycotopia.net/showthread.php?t=7476 (Psilocybe caerulipes)
by mushpuppet, posted be Waylit.

Do you think that pasteurizing the river sand would be a benefit?

[golly]

U got one Coyote..PM yer addy...
Any of the usual Agar formulas should work ,although have not tried with these..
You should be able to go direct to spawn too ,,,Whole grain flour coated wood chips, or even PF tec to start..
Any of the softer hardwoods should colo fast ,but did read one report about Aspen not working...

[CoyoteMesc]

pm sent,
Thanks so much.

any opinion on the pasteurizing

*droooooools*

[golly]

CM/Foster, prints have been sent...

Well the first taste test is in...1gram produced a buzz slightly stronger than a
Cube,maybe 1.3 X ...The main difference is the smooth and pleasant body effect ,A more sophisticated [for lack of a better word] experience..
Good light visuals..Definately worth exploring more deeply..heh...

I have noticed that pinning occurs more with sub temps in the 60s..Cause when i brought a couple indoors where it's 74F ,no new pins formed until i moved em back out in the cooler GH...Could be a co-inkydink but may be worth trying ..

[CoyoteMesc]

*temps noted*

A more sophisticated [for lack of a better word] experience..

Sounds like Mermaidia may love that...

So was that dried or 13grams fresh?
I'm assuming dried.

[al-cabar]

Err.. Looks like I'm kinda late, yet I've send a request for a print 2 weeks ago.
I was going to make a plantation of Caerulipes in the nearby forest.
And I've already chopped a cherry-tree to use it as a substrate!

[Foster]

Cool Golly! Pinning info noted. Mother Nature must be listening too. Temps being a lil warmer than usual, seem perfect for colonization atm, and will hopefully drop back to normal for a nice pinning enviroment. great stuff

foster

[max]

I'll just mention that we still have this specie available at the SporeLab. I don't know if any other vendors have it or not....

[golly]

That was 1 dry gram CM...No blueing was noted on cut stems,so they maybe good keepers...
The temp in the outdoor bed is near 55F now, so i'm thinking around mid April they may begin popp'n...

[mycobri]

were those mini-tubs exposed to rainwater at all?

i'm loving that last picture of the fully opened caps

[golly]

just untreated pond water ...

[waylitjim]

Those are beautiful specimens Golly!
You do good work my friend.

[golly]

Just a pic of tall one and a dark one..The stem bends to the right at the bottom ,making it almost 7" long...

[Freaky]

Thank you for sharing this Golly! You're amazing!

[CoyoteMesc]

Golly, do you think that it would be worth my time to iso. my print on agar first. Or would that just be a bit of a waist.
Also if I do use a dish, should I sector it the same as pans or cubies? I know this may sound like a dumb question and my guess would be to treat it the same. But I have never worked with a wood/cold loving species

[golly]

I would recommend using a small part of the print for direct grain spawn culture and an even smaller [tiny] amount for agar..
Yes , sector out the most agressive myc for expantion...The prints should be fairly clean...I went direct to spawn with no problem...

It's my opinion that fruiting would be enhanced by cloning/ isolation ..With cloning being the most reliable method..

[dial8]

Wow, golly, I'm really impressed. Beautiful grow and beautiful fruit!

very cool mushrooms you have there golly!

[golly]

The new clones seem to be taking off , on wood mulch and cardboard...

Tried another dose ,this time 1.25g and was much stronger in effect.
Equal to 3 grams o' Cubes.. ..Again, a very nice, tight trip that does not linger on, like with Panaeolus...

[llamabox]

Hey Golly, I have taken up to 4-5 grams dried of the bluefoots and have found they are still quite mellow, "sophisticated", at these high doses.

[golly]

Heh ..Don't think i'll be going there any time soon, thinking 1.5g would be a perfect dose for a short, brilliant colorfull adventure..
The flavour is very similar to Cubes but not as strong...

[llamabox]

There is just something odd about bieng in the woods and having the guy next to you smacking his lips, making sickening smooching sounds and dribbling blue drool while snacking on the first fresh bluefoot.

Ah the joys of spring.....

[CoyoteMesc]

My buddy has decided to let a few spores meet up in a LC. My question; what temps would be best for this species while incubating in the liquid?
Thanks in advance,
CM

[golly]

They colo well at 65-77F

[llamabox]

I would agree with Golly. They seem to be sensitive to higher temps and I have had a few stall out rather quickly and contam at temps in the 80's.

[fahtster]

awesome golly! whos da man? yous da man! beautiful freakin pics heh.

fahtster

[golly]

Here's a look at a Bluefoot clone, about a week old..

The method seems to work well for this woodlover..

In a small plastic tray with drain holes , hydrated shredded wood mulch was boiled/drained, then placed inside..

A whole mushroom was dropped into a blender with a halfcup distilled water
and chopped up..

The fragged mush/water was then poured onto the shredded mulch and allowed to drain out..

The tray is then partially covered ,maybe 80% ..After a few days when it began to dry a little , it was very lightly misted..
The fresh air is essential to prevent molds from germinating...

When this tray is more colo'd - it will be spawned to a larger batch of mulch n' chips..It'll proly take a couple months to expand enough for fruiting...

[mycospora]

The species in question is Psilocybe ovoideocystidiata.

It is close to P. stuntzii microscopically. And while mushpuppet wrote of small differences, there are more than just the annulus on the mushrooms.

Here is the paper minus the illustration and line drawings since I do not know how to post a picture here unless I take one from another site.

So this is the article on the shroom, which is a separat e species fromthat of the p. caerulipes.

International Journal of Medicinal Mushrooms, Vol. 9, pp. 75–77 (2007)<o></o>
1521-9437/07/$35.00<o></o>
© 2007 by Begell House, Inc.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
New Species of Hallucinogenic Psilocybe (Fr.) P. Kumm.<o></o>
(Agaricomycetideae) from the Eastern U.S.A.<o></o>
Gastón Guzmán,1 Richard V. Gaines,2 and Florencia Ramírez-Guillén1<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
ABSTRACT: Psilocybe ovoideocystidiata is described as a new blueing species from Pennsylvania,<o></o>
USA. It belongs to section Stuntzii Guzmán of genus Psilocybe for its subrhomboid, thick-walled spores and its caerulescent basidioma with annulus.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
KEY WORDS: hallucinogenic and blueing species, Psilocybe, sect. Stuntzii, USA, ecology, geography

<o></o>
Electronic Data<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
INTRODUCTION<o></o>
Through several explorations in a Pennsylvania State<o></o>
XXXX in the United States since the spring of 2003<o></o>
made by one of the authors (Gaines), we found a<o></o>
blueing Psilocybe that is herein described as a new<o></o>
species. It is interesting to observe that, although the<o></o>
genus Psilocybe began to be studied in the United<o></o>
States in 1872 by Peck (1872, 1912), and in 1958 it<o></o>
came to attention with the discovery of the hallucinogenic<o></o>
species (Singer and Smith, 1958)—which<o></o>
Guzmán (1983, 1995, 2000) added to the known<o></o>
species found in the United States—and recently<o></o>
Guzmán and Trappe (2005) and Guzmán et al. (1997,<o></o>
2003) described new species from the United States,<o></o>
we nevertheless found yet another new species. There<o></o>
are in the United States around 60 species of Psilocybe,<o></o>
of which approximately 25 are hallucinogenic, and of<o></o>
which around 10 are from the eastern United States<o></o>
(Guzmán et al., 1997, 2003; Guzmán, 2005).<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
MATERIALS AND METHODS<o></o>
Microscopic observations were made through handle<o></o>
sections of dry basidiomata, mounted in 5% KOH or<o></o>
5% NH4OH solutions or both, mixed with 1% Congo<o></o>
Red solution, previously treated with 96% alcohol for<o></o>
rehydrating of the tissues. The size of spores is long<o></o>
and wide on face view and thick on side view.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
RESULTS<o></o>
Psilocybe ovoideocystidiata Guzmán et<o></o>
Gaines, sp. nov. (Figs. 1–7)<o></o>
Pileus (10–) 15–25 (–45) mm latus, convexus vel<o></o>
subumbonatus, glaber, subviscidus, hygrophanus,<o></o>
aurantiacus brunneus vel fulvus. Lamellae subadnatae,<o></o>
subfuscus violaceous, marginis concolor. Stipes<o></o>
(15–) 25–60 (–90) × (1–) 2–5 (–7) mm, albidus,<o></o>
caerulescente. Annulus membranaceus. Sporae (7–)<o></o>
8–9 (–12) × (5.5–) 6–7 (–8.5) ìm, rhomboideus vel<o></o>
sub-rhomboideus, crassotunicatae, poro germanativo<o></o>
praeditae. Pleurocystidia duobus typis, a: 16–24<o></o>
(–35) × 6–8 (–10) ìm, hyalina, ventricose rostrata;<o></o>
et b: 20–30 (–40) × (10–) 12–16 (–20) ìm, pallidus<o></o>
brunneus griseolus, globose pyriformis vel ventricose<o></o>
clavatus. Cheilocystidia duobus typis, a: 18–22 × 5–<o></o>
9 (–11) ìm, hyalina, ventricose rostrata; et b: 25–29<o></o>
× 9–13 ìm, hyaline vel inaequalis cyaneus, globousus,<o></o>
peduncule vel subpenduncule. Pileipellis ixocutis<o></o>
crassa. Hyphae fibulate. Species lignicola vel sub-lignicola,<o></o>
sylva temperatus deciduous. Holotypus:<o></o>
USA, 51b, XAL.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
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<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
FIGURES 1–7. Psilocybe ovoideocystidiata, 1: four basidiomata, showing convex to subumbonate, hygrophanous pileus, the annulus, and the smooth to scaly, subbulbous and hollow stipe, 2: spores, 3: basidia, 4: cheilocystidia type a, 5: cheilocystidia type b, 6: pleurocystidia type a (that on the right is uncommon), 7: pleurocystidia type b (those with neck are uncommon) (all from the holotype). Scale bar 1: 10 mm, 2–7: 10 ìm.
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
Etymology: From the Frequent Ovoid Both<o></o>
Pleuro- and Cheilocystidia<o></o>
Pileus (10–) 15–25 (–45) mm diam., convex to<o></o>
subumbonate, lubricous to subviscid, glabrous, translucent<o></o>
striate at the margin, hygrophanous, orangish<o></o>
brown to yellowish brown, sometimes white when<o></o>
dry. Lamellae subadnate, brownish pale to dark<o></o>
brownish violaceous, uniform in color. Stipe (15–)<o></o>
25–60 (–90) × (1–) 2–5 (–7) mm, smooth above to<o></o>
floccose-scaly below, cylindric, equal, somewhat<o></o>
subbulbous, base sometimes hypogeous, whitish,<o></o>
with irregular pale ochre or violaceous tones below<o></o>
or pale reddish brown above, hollow, with white<o></o>
mycelium at the base. Annulus membraneous, white,<o></o>
evanescent. Context whitish to ocherous pale, blueing,<o></o>
odor farinaceous. Spore print violaceous dark.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
Spores (7–) 8–9 (–12) × (5.5–) 6–7 (–8.5) ìm,<o></o>
rhomboid or subrhomboid in face view, subellipsoid<o></o>
in side view, thick walled, wall 0.8–1.5 ìm thick,<o></o>
yellowish brown, with a broad germ pore at one end<o></o>
and a short appendage at the other. Basidia 20–28<o></o>
× 7–9 ìm, 4-spored, clavate-ventricose, sometimes<o></o>
with a middle constriction, hyaline. Pleurocystidia<o></o>
of two types, a: short, 16–24 (–35) × 6–8 (–10) ìm,<o></o>
hyaline, ventricose-rostrate, with an acute or broad<o></o>
base; b: large, 20–30 (–40) × (10–) 12–16 (–20)<o></o>
ìm, brownish gray pale, globose-pyriform, sometimes<o></o>
with a narrow or moniliform apex, and has a<o></o>
large narrow base. Cheilocystidia of two types, a:<o></o>
short, 18–22 × 5–9 (–11) ìm, as pleurocystidia type<o></o>
a; b: 25–29 × 9–13 ìm, globose, pedunculate or<o></o>
subpedunculate, hyaline or with a bluing irregular<o></o>
content. Subhymenium subcellular, brownish pale.<o></o>
Hymenophoral trama regular, with hyphae hyaline<o></o>
to yellowish in mass, 3–18 ìm wide. Pileipellis an<o></o>
ixocutis, 90–100 ìm thick, with hyaline, thin-walled<o></o>
hyphae, 2–5 ìm wide. Pileus trama with hyaline to<o></o>
yellowish in mass hyphae, 4–18 ìm wide. Basal <o></o>
mycelium with hyaline, thin-walled hyphae, 1.5–<o></o>
5 ìm wide. Clamp connections present.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
Habitat and Distribution<o></o>
Gregarious, on wood or wood debris, in trails or<o></o>
places with herbaceous plants, in a deciduous forest.<o></o>
Known only from the type locality.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
Studied Specimens<o></o>
USA, PennsylvaniaJune 5, 2005,<o></o>
Gaines 51b (holotype XAL, isotype NY and Gaines<o></o>
Herb.); June 6, 2005, Gaines 50a; June 22, 2005,<o></o>
Gaines 52a, 53b (all in XAL and Gaines Herb.).<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
Discussion<o></o>
This species is close to Psilocybe subaeruginascens<o></o>
Hohnel from Java, P. septentrionalis (Guzmán)<o></o>
Guzmán from Japan, and P. wayanadensis K. A.<o></o>
Thomas, Manim. et Guzmán from India (Guzmán,<o></o>
1983; Thomas et al., 2002), for the rhomboid or<o></o>
subrhomboid, thick-walled spores, and the annulus<o></o>
and the blueing feature of the basidioma. All these<o></o>
species belong to the section Stuntzii Guzmán,<o></o>
following the classification of Guzmán (1983,<o></o>
1995). Psilocybe ovoideocystidiata differs for the<o></o>
two types of wide pleurocystidia and cheilocystidia.<o></o>
For the blueing feature, this species probably has<o></o>
hallucinogenic properties, following Guzmán’s<o></o>
(1983) criterion.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
ACKNOWLEDGMENTS<o></o>
The senior author and F. Ramírez-Guillén acknowledge<o></o>
Etelvina Gándara, Juan Lara Carmona, and<o></o>
Manuel Hernández of Instituto de Ecología at<o></o>
Xalapa for their valuable help in the laboratory, in<o></o>
the herbarium, and on the computer, respectively.<o></o>
The senior author also thanks Instituto de Ecología<o></o>
and CONACYT for their support of his research.<o></o>
This article was reviewed by Laura Guzmán-<o></o>
Dávalos of the Instituto de Botánica, Universidad<o></o>
de Guadalajara, Mexico.<o></o>
<!--[if !supportEmptyParas]--> <!--[endif]--><o></o>
REFERENCES<o></o>
Guzmán G. 1983. The genus Psilocybe. Beihefte zur<o></o>
Nova Hedwigia 74, Cramer, Vaduz, Germany. 439<o></o>
pp. + 40 pls.<o></o>
Guzmán G. 1995. Supplement to the monograph of<o></o>
the genus Psilocybe. In: Taxonomic Monographs<o></o>
of Agaricales, Petrini O. and Horak E., eds.<o></o>
Bibliotheca Mycologica 159, Cramer, Berlin,<o></o>
pp. 91–141.<o></o>
Guzmán G. 2000. New species and new records of<o></o>
Psilocybe from Spain, the U.S.A., and Mexico, and<o></o>
a new case of poisoning by P. barrerae. Documents<o></o>
Mycologiques, 29, 41–48.<o></o>
Guzmán G. 2005. Species diversity of the genus<o></o>
Psilocybe in the world mycobiota, with special<o></o>
attention to hallucinogenic properties. Int J Med<o></o>
Mushr, 7, 305–331.<o></o>
Guzmán G., Hanlin R. T., and White C. 2003. Another<o></o>
new species of Psilocybe from Georgia,<o></o>
U.S.

mycospora
<o></o>
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[golly]

Thanx for the info Myco..It doesn't suprize me that these may be another species..Caerulipes are reported to have chocolate spores i think..Mine are much lighter brown...But what a name,,,I can't live with that..heh..
I think these originated in Indiana/Illinois ...