Woodlover-Regeneration-TEK: Densification of Rhizomorphs by Layering - Page 3

little white rabbit · 10-24-07, 14:49

@jake: thx for the info about average alcaloid content of p. azurescens. was searching for something like this some time now.
@rhizo: great pics as usual

totallynew · 10-24-07, 14:50

Great pictures. I'm also a photographer and these pictures are beautiful!

I'm incredibly envious of your growth! Congrats.

Have you ever though of writing in this up in a very simple/easy to follow/illustrated how to?

Rhizoo · 10-24-07, 17:57

Quote:

Originally Posted by Jake420

After a bit of searching through texts, books and the net I was able to get the percentage of alkaloids (all sources held the concentrations of Psilocybin, Psilocin and Baeocystin which were then added together) from almost two dozen different tests on Psilocybe azurescens samples. With a bit of compiling and calculating, the average alkaloid content for azurescens came out to be 2.056%. That is the average potency. The highest of that group was 2.51% and the lowest cencentration was 1.61%. One word. Sweet. Or maybe... Beautiful. Well above the average of any other mushroom potency, and that lowest percentage is what many mushrooms barely compare to at their highest potency. Nifty bit of information hmm?

Have fun peeps!

Hi Jake, sorry for nitpicking

but is this the average of the highest concentrations quoted in different texts, or the average of the average concentrations? I ask because Stamets quotes 2.51% as the highest concentration ever found in a single azurescens (but does not quote the average), and building the average of maximums would not really be that meaningful (because a maximum value just depends on the date of publication - the older the less)...

Quote:

Have you ever though of writing in this up in a very simple/easy to follow/illustrated how to?

Isn't it already?

Well, I thought about including a table of contents and a special pointer to the conclusions of the first experiment (which give a good idea of what it is all about and what to do), but I can't edit my first post anymore...

Jake420 · 10-24-07, 18:48

The lowest concentration of alkaloids published that I could find I included here. The average that I listed is from ALL published concentrations of alkaloids I could find within twenty minutes. Like I said, interesting information there. Enjoy!

waylitjim · 10-27-07, 02:27

2 dry grams of dry azures is a good dose. It's powerful, but not debilitating. 20 grams fresh is even better, it adds a little sparkle which isn't there once they dry. Man, it's hard to beat the high of woodlovers. Ya gotta love em'

Rhizoo · 10-28-07, 13:29

Damn, I've to wait for the christmas holiday so I have the time for a full dose... But will try 0.5g some sunday afternoon...

In the rest of the patch some nice thick azures grew. Those in the dense clusters were smaller and thinner most of the time.
I'll let one or two of them stay there to document the ripening and the final decay...

The mycelium at the base is really amazing

Gills & Stem

Gills & no Stem

MrMycelium · 10-29-07, 11:32

Absolutely awesome pics as always.

Rhizoo · 10-29-07, 17:40

Thanks

btw, try the following:

stare at the last picure for some time.
look at a wall

MrMycelium · 10-30-07, 11:04

Nice, i made it my wallpaper.

Rhizoo · 10-31-07, 08:58

The last "normal" ones from the azurescens patch

now only those to document the decay remain in the patch...

waylitjim · 10-31-07, 12:46

Hey Rhiz... how did you prep your woodchips for the containerized experiments?
Bleach soak, pasteurize, sterilize ? Any problems with contams ?

Rhizoo · 10-31-07, 20:57

Quote:

Originally Posted by waylitjim

Hey Rhiz... how did you prep your woodchips for the containerized experiments?
Bleach soak, pasteurize, sterilize ? Any problems with contams ?

I usually boil the chips until they are so soaked that they sink (15min or so), pour everything through a colander, cover the colander until the chips have cooled down (a hour or so), and then use them. Since I have no PC (yet), I can't compare to sterilizing, but I guess the chips I use are rather sterile from the beginning because they were heat dried and contain not much bark. Didn't try bleach yet.

I see contams rather seldom (and even if (e.g. because a layer was too thick), in a limited area so the contamnd chips can be removed easily). But as I said, the idea here is to adjust the thickness of new layers according to both the strength of the mycelium and conditions which might favour contamns (like nutrients, sporecount in the air, contamns already present in the wood, high temperature and so on), so that the mycelium can reach the surface before contamns can establish themselves. Never had a contamn which contaminated colonized wood.

Rhizoo · 11-01-07, 09:47

Hmm, something is growing in a flower box, finally. I hope these are real cyans, and not again azures

Damn, clusters again

waylitjim · 11-01-07, 12:03

Bring on the cyans Rhizoo!
I'm ready for round two.

fedshtkpndrk · 11-01-07, 12:31

Much Envy

MrMycelium · 11-01-07, 20:58

Round 2..... ok let me get a towel to wipe up all the drool i am going to have.

hyphaenation · 11-01-07, 21:10

Not bad for a "first time grower" ....

understatement

Myco-Iggy · 11-01-07, 23:40

Simply Stunning!

Rhizoo · 11-04-07, 08:53

Round 2: slowly the cyan advance, only in one box so far...

Statler and Waldorf are still in great shape

btw, don't let...

...those close-ups fool you

(dimensions of the dice: 16mm/0.6inch)

Jake420 · 11-04-07, 12:05

Nifty. Nice comparison Rhizoo. Heh, amazing how small they start isn't it?

hyphaenation · 11-04-07, 12:37

Quote:

Statler and Waldorf are still in great shape

Now thats putting your heads together...

Rhizoo · 11-07-07, 13:48

A little update:

Did they grow or did they not, that's the question

The "biggest" ones so far...

Tried light from different directions and could not decide on one pic, so I just post both

OG Royal Grower · 11-07-07, 19:54

Wonderful work! Sooo Beautiful, great pictures what kind of camera are you using?,
OG Royal Grower

This one reached a place in the sun...

[/quote]

Rhizoo · 11-11-07, 14:58

@OGRG: I use a rather cheap Bridge/Superzoom model. I explained my workflow somewhere on page 2.

Since its raining constantly, I didn't make photos of the old azures this week, only indoor photos of the box.

Cyans, cont'd

Some new pins at the same edge as the others. Seems to be a one-edge-fruiter...

MrMycelium · 11-11-07, 21:42

Simply awesome.

Rhizoo · 11-12-07, 21:18

Statler & Waldorf, after a week of rain

Who ... is this?!

This mushroom I found in a cyan patch I have (same cyan as in the boxes), where until now I looked unsuccessfully for pins or even knots forming. I guess I've overlooked this one because of its sheer size...

It grew at a edge of the cyan patch, rather hidden behind a shrub and in a little pile of fallen leaves. At first I didn't even recognize it and wanted to throw it away

It resembles much more a azure than the cyan I had expected at this spot.

Cyans should be smaller, at least that's what I read. (Note: these are cm not inch

)

The sporeprint has the same tint as other Ps. And tearing the stem apart smelled like with other Ps.

It seem my "cyan" is more azure-like. This mycelium stems from a spawnbag from an european vendor. This year there were several reports in forums that spawnbags were mislabeled in in one way or the other. The development of the pins in the box will hopefully give more insight what it really is.

Oh no, Azures again

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10-24-07, 14:49	#101 (permalink)
little white rabbit Mycotopiate Join Date: Sep 2007 Posts: 239	@jake: thx for the info about average alcaloid content of p. azurescens. was searching for something like this some time now. @rhizo: great pics as usual __________________ I've been a wild rover for many's the year and I've spent all my money on whiskey and beer.

10-24-07, 18:48	#104 (permalink)
Jake420 The Mycoman Join Date: Aug 2007 Posts: 335	The lowest concentration of alkaloids published that I could find I included here. The average that I listed is from ALL published concentrations of alkaloids I could find within twenty minutes. Like I said, interesting information there. Enjoy! __________________ Doing all he can with his mycoplan.

10-27-07, 02:27	#105 (permalink)
waylitjim Grateful Mod Join Date: Sep 1972 Posts: 3,936	2 dry grams of dry azures is a good dose. It's powerful, but not debilitating. 20 grams fresh is even better, it adds a little sparkle which isn't there once they dry. Man, it's hard to beat the high of woodlovers. Ya gotta love em' __________________ <((( Waylit's Pan cyan Tek ~~~ Waylit's Wood Lover Tek )))>

10-29-07, 11:32	#107 (permalink)
MrMycelium Breeder of Fungus Gnats Join Date: Aug 2007 Posts: 973	Absolutely awesome pics as always. __________________ Last night I lay in bed looking up at the stars in the sky and I thought to myself, where the heck is the ceiling.

10-24-07, 14:50	#102 (permalink)
totallynew Former Member Join Date: Mar 2007 Posts: 71	Great pictures. I'm also a photographer and these pictures are beautiful! I'm incredibly envious of your growth! Congrats. Have you ever though of writing in this up in a very simple/easy to follow/illustrated how to?

10-29-07, 17:40	#108 (permalink)
Rhizoo Mycophage Join Date: Sep 2007 Posts: 124	Thanks btw, try the following: stare at the last picure for some time. look at a wall __________________ Woodlover-Regeneration-TEK: Densification of Rhizomorphs by Layering

10-30-07, 11:04	#109 (permalink)
MrMycelium Breeder of Fungus Gnats Join Date: Aug 2007 Posts: 973	Nice, i made it my wallpaper. __________________ Last night I lay in bed looking up at the stars in the sky and I thought to myself, where the heck is the ceiling.

10-31-07, 12:46	#111 (permalink)
waylitjim Grateful Mod Join Date: Sep 1972 Posts: 3,936	Hey Rhiz... how did you prep your woodchips for the containerized experiments? Bleach soak, pasteurize, sterilize ? Any problems with contams ? __________________ <((( Waylit's Pan cyan Tek ~~~ Waylit's Wood Lover Tek )))>

11-01-07, 12:03	#114 (permalink)
waylitjim Grateful Mod Join Date: Sep 1972 Posts: 3,936	Bring on the cyans Rhizoo! I'm ready for round two. __________________ <((( Waylit's Pan cyan Tek ~~~ Waylit's Wood Lover Tek )))>

11-01-07, 12:31	#115 (permalink)
fedshtkpndrk Pantastic Mod Join Date: Mar 2007 Posts: 1,049	Much Envy

11-01-07, 20:58	#116 (permalink)
MrMycelium Breeder of Fungus Gnats Join Date: Aug 2007 Posts: 973	Round 2..... ok let me get a towel to wipe up all the drool i am going to have. __________________ Last night I lay in bed looking up at the stars in the sky and I thought to myself, where the heck is the ceiling.

11-01-07, 21:10	#117 (permalink)
hyphaenation Moss Walker Join Date: Feb 2007 Posts: 4,046	Not bad for a "first time grower" .... understatement __________________ [10:52 am] Beastmaster: thats a waste of good drugs if all you're gonna do is hide in bed afraid of shadows

11-01-07, 23:40	#118 (permalink)
Myco-Iggy Psychonaught Join Date: Nov 2006 Posts: 21	Simply Stunning! __________________ "This body holding me reminds me of my own mortality. Embrace this moment, remember, We are eternal, All this pain is an illusion."

11-04-07, 12:05	#120 (permalink)
Jake420 The Mycoman Join Date: Aug 2007 Posts: 335	Nifty. Nice comparison Rhizoo. Heh, amazing how small they start isn't it? __________________ Doing all he can with his mycoplan.

11-07-07, 19:54	#123 (permalink)
OG Royal Grower Mycophiliac Join Date: Feb 2007 Posts: 88	Wonderful work! Sooo Beautiful, great pictures what kind of camera are you using?, OG Royal Grower This one reached a place in the sun... [/quote]

11-11-07, 21:42	#125 (permalink)
MrMycelium Breeder of Fungus Gnats Join Date: Aug 2007 Posts: 973	Simply awesome. __________________ Last night I lay in bed looking up at the stars in the sky and I thought to myself, where the heck is the ceiling.