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  • Go Back   Mycotopia Web Forums > Deep Knowledge > The new Vaults > FAQ : Frequently Asked Questions

     
     
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    Old 01-16-06, 20:25   #1 (permalink)
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    Waylit's WoodLover Tek

    This thread was created to take some of the mystery out of growing woodlovers. The process is highly enjoyable and with a little patience, so are the fruits of your labor. The methods demonstrated here can be applied to Psilocybe cyanescens, P. azurescens, P. subaeruginosa and other temperate and tropical woodloving species.

    This thread contains four main sections:

    I. Building Spawn using Grains

    II. Creating an Outdoor Bed

    III. Making a Woodlover Liquid Culture

    IV. Creating a Fruiting Substrate without Grains

    _____________________________________________

    Part I ~ Building wood chip spawn using grains.

    Supplies needed:

    -A tub lined with corrugated cardboard.

    -Hardwood chips, I'm using alder, but most hardwoods will work. To prepare the wood chips, soak them overnite in bleach water (1.25 tbsp bleach per gallon.) They do not need to be pasteurized or sterilized.

    -4 jars of spawn. I used the standard PF Tek recipe. (But other grains can also be used.)





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    Old 01-16-06, 20:25   #2 (permalink)
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    Add a 2 inch layer of soaked alder chips ontop of the cardboard and spread them out evenly.


    Crumble 1 of you BRF jars inside a ziplock bag.


    Spread this spawn evenly across the wood chips.


    Add a second layer of wood chips covering the BRF.
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    Old 01-16-06, 20:25   #3 (permalink)
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    For the middle layer, I use 2 BRF cakes.


    Crumble the cakes and spread em' evenly across the wood chips.


    Cover with a fresh layer of chips.



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    Old 01-16-06, 20:25   #4 (permalink)
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    Take your last jar of spawn and mix it in with the top layer of wood chips.





    Cover the tub with aluminum foil and poke a few holes for air exchange.


    Set your tub aside to colonize for the next couple months.
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    Old 01-16-06, 20:25   #5 (permalink)
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    One week later you should see some new growth. The mycelium will spread out and colonize the entire tub of chips. Once this spawn is mixed with your outdoor garden, the growth will be explosive. Following this method you can make many tubs and many gardens.



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    Old 01-16-06, 20:32   #6 (permalink)
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    Part II ~ Creating an Outdoor Bed

    While your wood chip spawn is colonizing, it's time to create your outdoor bed.

    1. Buy some bulk alder chips.



    2. Soak them over night in large tubs to achieve proper hydration.



    3. Choose a shady spot in a safe location and lay down cardboard cut to the shape of your plot.

    4. Cover the cardboard with a fresh layer of soaked alder chips.



    5. Grab one of your fully colonized tubs and dump it out. Spread out the colonized chips and mix in some nice organic soil. Add another layer of freshly soaked chips.



    What you're trying to do is layer wood chips/soil, spawn, wood chips/soil, spawn, untill your bed is 6 inches deep. It's helpful to build a wooden frame around the bed.
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    Old 01-16-06, 20:32   #7 (permalink)
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    Organic soil is mixed in with each layer of fresh wood chips. The soil is an important part of fruiting azurescens and cyanescens. The mycelium needs the microbial activity to form healthy mushooms.
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    Old 01-16-06, 20:40   #8 (permalink)
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    Un-pasteurized shredded straw was also mixed in with the chips, soil and spawn. The straw helps to retain moisture and keep the bed aerated. We're almost done!





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    Old 01-16-06, 20:41   #9 (permalink)
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    For a finishing touch, rhododendrons were planted.

    The rhododendrons will provide some shade and give the garden a "normal" look.



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    Old 07-10-06, 18:44   #10 (permalink)
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    Part III ~ Making a Woodlover Liquid Culture

    This is how I make my woodlover liquid cultures. The process starts with soaking hardwood chips in water over nite. The water absorbs nutrients from the wood chips which makes an excellent broth. The tea is poured thru a coffee filter to strain out any small particles, in this case hickory chips were used. 250 ml of hickory tea was added to the baby bottle and 1 tsp of Karo was added. Here's some of the supplies.



    I used a baby bottle for the LC. The top is snipped off, and the nozzle is stuffed with polyfill.



    The tea is microwaved for 3 minutes. After it cools, an azurescens print is added.
    These pics show the growth 48 hrs later.





    The LC can be used to create your final fruiting substrate.



    If all goes well, we'll have another pretty vase of azures.



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    Old 07-10-06, 19:18   #11 (permalink)
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    Great innovative tek waylit!! I must admit to lurking for some time over your threads, I am just so curious about woodlovers. I've never even eaten any yet! Could you clone an azurescens into that tea? I'm just wondering if it's possible as the stems are so small, I'm not making a request (or am I??)
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    Old 07-10-06, 19:26   #12 (permalink)
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    Wow ,,,doesn't get simpler than that...So just 3 mins of nuking is enough..?
    May have to give 'er a go....
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    Old 07-10-06, 19:34   #13 (permalink)
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    Talking Facking Egggggcellent i tell ya!!!!!!!!!!!!!!

    "WOW"what a KILLER of a thread,
    you never cease to amaze Jim.
    Looks like i am going to have to give
    this a try real soon
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    Old 07-10-06, 19:44   #14 (permalink)
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    How do you microwave an lc for 3 mins without it boiling over?
    My microwave seems to boil lc way earlier than that. Even stopping it, swirling the liquid, and restarting it will still boil over.
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    Old 07-10-06, 19:55   #15 (permalink)
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    Have you tried this with other wood lovers as well?
    Say the Ps. caerulipes.....
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    Old 07-10-06, 19:57   #16 (permalink)
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    Quote:
    Originally Posted by CannAmerican
    Could you clone an azurescens into that tea?
    You can definitely clone fruits in a LC. This is a nutrient rich solution, so spores and tissue will grow out very fast. Prime sites for tissue extraction are the thick tissue at the base of the stem, and the fleshy tissue inside the cap above the gills. Tear the cap or the stem in half (not cutting, the blade can be a vector for contamination) and a small piece of inner tissue is carefully extracted (with a flame sterilized blade). Make sure you touch and extract nothing but clean tissue from inside the mushroom. If you use the base of a stem, be sure to extract tissue at least a half inch above the base to avoid contaminated tissue that is in contact with the substrate. The excised tissue is then dunked in a 10% peroxide dilution (10ml 3% diluted with 90ml sterile water), and transferred to the woodchip/ karo tea.

    Quote:
    Originally Posted by golly
    Wow ,,,doesn't get simpler than that...So just 3 mins of nuking is enough..? May have to give 'er a go....
    I've played around with varying lengths of time. With my microwave on low, the solution is boiling after 1 minute. So the LC gets 2 minutes of boiling. I've had no contamination problems with this technique. I tried 5 minutes, but it seemed like overkill. The solution would usually boil over after 3 minutes.
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    Old 07-10-06, 20:05   #17 (permalink)
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    Quote:
    Originally Posted by llamabox
    Have you tried this with other wood lovers as well?
    Say the Ps. caerulipes.....
    I just finished a LC with Psilocybe subaeruginosa. I'm sure any woodlover would love this brew.

    Quote:
    Originally Posted by shobimono
    How do you microwave an lc for 3 mins without it boiling over?
    My microwave seems to boil lc way earlier than that. Even stopping it, swirling the liquid, and restarting it will still boil over.
    I have a slow cook feature on my microwave. It's under the low setting. After 1 minute, it reaches a slow rolling boil. By 3 minutes it's boiling rapidly. I only fill the bottle 3/4 way up so the poly fill doesn't get wet.
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    Old 07-11-06, 22:41   #18 (permalink)
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    Hey jim how do you normaly prep your wood chips?

    ...couldnt really find any info
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    Old 07-12-06, 00:10   #19 (permalink)
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    I soak the chips in water for 24 hrs. or untill they're fully hydrated. Rarely do I pasteurize or sterilize the woodchips. I do add bleach to the soak water to kill off any molds or endospores living on the wood.
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    Old 07-12-06, 10:04   #20 (permalink)
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    Very nice tek Waylit. I'm bookmarking this.
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    Old 07-12-06, 10:40   #21 (permalink)
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    wow! hey waylitjim, that is about as easy as it gets! great thread and tek! With a little more current experience I just might give ere a go. Truly Sweet! peace pFost
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    Old 07-12-06, 14:04   #22 (permalink)
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    Nice tread ,..Waylitjim !

    I was just thinking what to do in my summer vacation...!

    Now I'm thinking of getting my Subaeruginosa print out of the closet and give your L.C. a try !
    Or maybe a Azure..but I don't have a print jet....
    ...O wait can try a Pan culture clone !
    We will see ! (so much to do..so little time...)
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    Old 08-07-06, 15:50   #23 (permalink)
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    While that does occur in nature , It's a reeeeall long shot ...So pretty much no it would not work dependably....
    Grain spawn or flour mixed with wood chips is the deal...
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    Old 08-07-06, 18:37   #24 (permalink)
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    Ok, got some popcorn soaking.
    I will look for other woodchips tomorow.
    Another question if I may:
    Would adding poo to wood be any good for them or should it be just wood?
    If so, what ratio?
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    Old 08-07-06, 18:54   #25 (permalink)
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    Manure will not help. If you're looking for a supplement try adding rye grass seed or bran to the woodchips. Oat, wheat, and rice bran in small amounts will boost your colonization times. 1-2 Tbsp per qt jar of woodchips is enough. Keep in mind, bran contaminates easily, so you'd need to pressure cook the chips and bran. I personally use rye grass seed and a nutritious cacuts soil with my wood chips.
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    Old 08-22-06, 19:23   #26 (permalink)
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    Part IV ~ Creating a Fruiting Substrate without Grains

    Here's some pics to demonstrate another way of using the wood lover liquid culture.

    Once the L.C has grown out, prepare some quart jars containing the final fruiting substrate.

    This process skips using grains which need to be spawned to the woodchips at a later date. This will save time since it skips a step.

    Basically we're injecting 6 ccs of azurescens liquid culture into each quart jars containing the final fruiting substrate.

    These are the ingredients:

    > 80% Alder Chips (screened in a colander to keep out larger pieces of wood)
    > 15% Cactus Soil
    > 5% Rye Grass Seed



    > Cactus Soil (composed forest products, peat moss, sand, earthworm castings, bone meal, blood meal, cottonseed meal, and alfalfa meal.) This nutritious mix will fully colonize.



    > Rye Grass Seed Used to boost colonization times.



    These ingredients were mixed together, moistened with water, and pressure cooked for 1 hour.

    After they cooled the jars were inoculated with 6ccs of L.C. These jars are now 4 weeks old and are showing some nice healthy growth. The next step will be transferring the jars to a tub for fruiting.







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