pins, problems & invitro pix

sandman · 04-27-05, 16:01

my Foaf in deep space orbit sent my these pics.

He says he "spawned" 2 qts of WBS to 1 qt of verm and cased in 1/4" verm. There have been some tiny fat pins for arround 4 or 5 days, and the number is rising but they arent getting much bigger. They are really fat and funny looking. Anyone know what could be causing this? is this what happens when you grow in no gravity?

DJoutbreak · 04-27-05, 16:10

ive gotten those b4 and gave them, some more water to knock down the mycelium growin around them seemed to help but id wait for hippie to answer that one

Lazlo · 04-27-05, 16:28

Just keep fanning and misting of the sides of the tub or tetarrium a few times a day. Don't directly mist pins at that stage of growth. Misting pins direct at that stage could stunt or abort growth.

sandman · 04-27-05, 16:40

these were not misted after pins showed. They are in a greenhouse set up so they should be getting air.

chill · 04-27-05, 23:24

Quote:

Originally Posted by sandman420

He says he "spawned" 2 qts of WBS to 1 qt of verm

Could this have anything to do with it? Never heard of spawning to straight verm before.

jjoj · 04-28-05, 02:23

i'm not sure, but they look pefectly normal to me.

i've had many pins that look like that and grow into nice mushrooms fine.

i do admit though, that spawning to vermiculite is a little odd. i would've added something nutritive to it, at least.

sandman · 04-28-05, 06:44

this is based on highflyers rez effect post in archives. it sounded kinda funky to me too, but it also sounded easy and stuff. The pins do look pretty normal its just that they arent taking of like i usually see.

sandman · 04-28-05, 11:14

On a second note, has anyone figured out what causes really hollow stems?

altered_states · 04-28-05, 11:40

Quote:

Originally Posted by sandman420

On a second note, has anyone figured out what causes really hollow stems?

I THINK that it is caused by overabundance of CO2/lack of fresh air.

Hippie3 · 04-28-05, 17:17

what's the rh ?

sandman · 04-28-05, 17:20

04-28-05, 18:22

I had some pins that took days and days to grow, but they were like 4th flush. Well all you can do now is wait and see what happens. If those pins don't do anything, more will probably form and mature.

Lazlo · 04-28-05, 18:26

90's too high for casings. Works good for cakes but, 70-75% rh works better for casings IMO.

sandman · 04-28-05, 18:33

there isnt any condensation on the casing so i figure its not TOO high. it actually varies from arround 75-90. depending on when the humidifier is going off.

sandman · 05-01-05, 17:58

why god why?

There is a good pinset, but they ALL just get about 1/4" and then stop...they dont really look abortish...but they arent growing

Any one have any ideas?

anticheffy · 05-01-05, 18:30

the substrate is too dry possibly, I'd try injecting water into the substrate

sandman · 05-01-05, 18:45

thanks cheffy. My foaf injected it with arround 100-150 ccs of water.

Think that its 'too late' for these pins to mature? Maybe is hould dunk it, i mean its nothing but verm and WBS so it should hold water good...i mean its suposed to be rez effect lmao...

anticheffy · 05-01-05, 19:04

more water tomarrow via injection then more the day after that, get a pint or two in there, my tubs realy take alot of water, I mean alot.

those pins might be ok , I wouldnt mess with them.

I am assuming those are bus tub size casings, hard to tell by the pics.

sandman · 05-01-05, 19:49

kitty litter trays

thanks a bunch cheffy

MurCurY · 03-05-06, 14:47

my tiny EQ pin on rye...took the jar out of the incubator cause of lack of room....it grew only this single pin.

Hippie3 · 03-05-06, 20:23

rye ?
uncased grain rarely does well at all...

SharkieJones · 03-05-06, 20:41

Transfer it into some bulk substrate.

MurCurY · 03-09-06, 19:19

yep...uncased rye. new pics coming in a few minutes.....it's much larger now...and a couple of new pins formed....

MurCurY · 03-09-06, 19:22

new pics

Jovy · 03-09-06, 22:05

arent all those other lil brown spots pins also?

G'luck

MurCurY · 03-10-06, 06:15

no joyvy....if you look at the larger version of the picture...those are rye grains. Some of the grains in the second set of pics under the new pin are grains that are right up against the glass so the mycellium keeps getting banged into the glass when i pick itup....the "cake" of grains shrunk so much...there's now a 1/2" gap between the middle of the cake and the glass...at the top it's probably closer to a full inch....at the bottom...it's still regular size.

Now for the question.....what to do with these? the grain that is...i was thinking of using it to spawn to some straw after i harvest the couple of mushies it has on it.

doodaa man · 03-10-06, 11:09

Watch out ! is that green i see on some of the grain ?

MurCurY · 03-10-06, 18:02

nope...it's blue. Some of the grains are VERY blue.

the_chosen_one · 03-10-06, 18:30

Quote:

Originally Posted by MurCurY

Now for the question.....what to do with these? the grain that is...i was thinking of using it to spawn to some straw after i harvest the couple of mushies it has on it.

Hmmm. Hip is right. Pure grain fruiting is rare. I'd clone the fruit and try to use the grain to innoc something. Wouldn't count on it taking off but it might. Casing may be even better yet. That shrinkage means she's gettin' thirsty. Back to that fruit tho. Clone it if you can. EQ that is that aggressive would proly make a great fruiting isolate. Maybe even as mean as that one in Blue Helix's avatar...man that thing is soooo sweet! Fruiting on grain like that is definitely something that would grab my attention. I've only seen it a few times in 10+ years and those ended up being great isolates for me.

MurCurY · 03-10-06, 18:55

rare...really?....

then let me show off a bit....

MurCurY · 03-10-06, 18:55

oh...this is all the same rye "cake"

the_chosen_one · 03-10-06, 19:12

Holy shit!!! Clone it. Breed it. And certainly PM me for a trade when you are ready!

MurCurY · 03-10-06, 19:36

lol...yeah...all those little pins sprung up overnight. I'm curious as to how big this one's gong to get....contest anyone?

BuckarooBanzai · 03-10-06, 21:40

I agree with TCO, dude. Clone that sucker and use it as a culture source.

How long has it been since you inoculated that jar?

MurCurY · 03-10-06, 21:47

innoculated on 1/6

the_chosen_one · 03-10-06, 22:01

Quote:

Originally Posted by MurCurY

innoculated on 1/6

Once again....Holy Shit!

the_chosen_one · 03-10-06, 22:17

That's pretty old. No wonder its fruiting. My hat is off to you tho. That jar was definitely sterile! Musta had a high water content to slow it down that much and then fruit like that. When did the jar fill in?

I would say let it finish invitro just to see how big they get. You can always clone it after harvest. I'd still clone it simply cuz it's one tuff son of b!!!

MurCurY · 03-11-06, 10:25

yeah...the jar was taking up space in my incubator and the other 4 qt's contammed with wet spot. These filled in in about 14 days and just sat there. Took them out and put them on my desk..kind of forgot about them. it's been cold in the house...it started to warm up the past week or so..today it's supposed to get up to 66oF a swing from a balmy 20 of last week. but i always keep it above 55 in the apartment. guess they liked the swing in temp. it's now got 4 little pins and the one big guy...which is growing real fast! if it keeps up this rate it will be hitting the top of the jar in a couple of days.

MurCurY · 03-11-06, 10:27

oh...and i found a single pin on the other jar.

BuckarooBanzai · 03-11-06, 19:55

The miracles of neglect-Tek! It's shocking how hearty these little guys are...

MurCurY · 03-12-06, 10:18

todays update.

Well...since it's been on my desk it's getting some wacky light. Last night it started to switch direction. Here's an updated pic. I dumped the dried out lose grain. I noticed the newer pins are growing sideways....not up....odd...

MurCurY · 03-12-06, 10:20

and pic of pins

Hippie3 · 03-12-06, 10:31

if you like invitro
it'd be worth cloning a few of the bigger pins

MurCurY · 03-12-06, 10:36

yeah...i thought of that.

Now say i clone this big guy..or one of the smaller big guys...
The clone would be genetically predisposed to fruit on grain?

Hippie3 · 03-12-06, 10:44

exactly, to pin uncased

MurCurY · 03-12-06, 13:26

very interesting...i'll let you know how i make out on that.

MurCurY · 03-13-06, 17:00

the big guy is being picked today...it fell over yesterday and opened up today.

Question: Can i still take a print from this mushroom? The jar hasn't been opened at all...it's covered by double layer tyvek.

As far as cloning...let me get this right....i'd crack open the cap and take a chunk with my sterile scalpel. dip it in peroxide (3%) for about 5 seconds...then agar or lc.

right? Could i take a chunk from the stem?

arezap · 03-13-06, 18:12

Quote:

Originally Posted by MurCurY

? Could i take a chunk from the stem?

Yes.

Hippie3 · 03-13-06, 18:48

rip [don't cut] the stem open
and cut chunk out of its' sterile interior

MurCurY · 03-13-06, 18:52

ok...but the rest of it sounds good?

I was going to drop the piece of stem into a jar of some grain....any suggestions? I got wbs, corn, rye, and pf cakes.

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04-27-05, 16:10	#2 (permalink)
DJoutbreak Mycophiliac Join Date: Mar 2005 Posts: 24	ive gotten those b4 and gave them, some more water to knock down the mycelium growin around them seemed to help but id wait for hippie to answer that one __________________ the idea of illegal plant and animals is ridiculous in the first place...Terence McKenna (ur spirit lives on)

04-27-05, 16:28	#3 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	Just keep fanning and misting of the sides of the tub or tetarrium a few times a day. Don't directly mist pins at that stage of growth. Misting pins direct at that stage could stunt or abort growth. __________________ How can you have any pudding, if you don't eat your MEAT?

04-27-05, 16:40	#4 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	these were not misted after pins showed. They are in a greenhouse set up so they should be getting air. __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

04-28-05, 06:44	#7 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	this is based on highflyers rez effect post in archives. it sounded kinda funky to me too, but it also sounded easy and stuff. The pins do look pretty normal its just that they arent taking of like i usually see. __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

04-28-05, 02:23	#6 (permalink)
jjoj Mycophage Join Date: Jun 1972 Posts: 128	i'm not sure, but they look pefectly normal to me. i've had many pins that look like that and grow into nice mushrooms fine. i do admit though, that spawning to vermiculite is a little odd. i would've added something nutritive to it, at least.

04-28-05, 11:14	#8 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	On a second note, has anyone figured out what causes really hollow stems? __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

04-28-05, 17:17	#10 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	what's the rh ? __________________ visit dial-a-hippie .

04-28-05, 17:20	#11 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	90 __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

04-28-05, 18:22	#12 (permalink)
~sporeaddik Guest Posts: n/a	I had some pins that took days and days to grow, but they were like 4th flush. Well all you can do now is wait and see what happens. If those pins don't do anything, more will probably form and mature.

04-28-05, 18:26	#13 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	90's too high for casings. Works good for cakes but, 70-75% rh works better for casings IMO. __________________ How can you have any pudding, if you don't eat your MEAT?

04-28-05, 18:33	#14 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	there isnt any condensation on the casing so i figure its not TOO high. it actually varies from arround 75-90. depending on when the humidifier is going off. __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

05-01-05, 18:30	#16 (permalink)
anticheffy Mycotopiate Join Date: Sep 1972 Posts: 1,038	the substrate is too dry possibly, I'd try injecting water into the substrate __________________ travel is fatal to predjudice, bigotry, and narrow mindedness mark twain

05-01-05, 18:45	#17 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	thanks cheffy. My foaf injected it with arround 100-150 ccs of water. Think that its 'too late' for these pins to mature? Maybe is hould dunk it, i mean its nothing but verm and WBS so it should hold water good...i mean its suposed to be rez effect lmao... __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

05-01-05, 19:04	#18 (permalink)
anticheffy Mycotopiate Join Date: Sep 1972 Posts: 1,038	more water tomarrow via injection then more the day after that, get a pint or two in there, my tubs realy take alot of water, I mean alot. those pins might be ok , I wouldnt mess with them. I am assuming those are bus tub size casings, hard to tell by the pics. __________________ travel is fatal to predjudice, bigotry, and narrow mindedness mark twain

05-01-05, 19:49	#19 (permalink)
sandman Harvester of Sorrow Join Date: Apr 1972 Posts: 1,970	kitty litter trays thanks a bunch cheffy __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

03-05-06, 20:23	#21 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	rye ? uncased grain rarely does well at all... __________________ visit dial-a-hippie .

03-05-06, 20:41	#22 (permalink)
SharkieJones Moderator Join Date: Feb 2005 Posts: 3,110	Transfer it into some bulk substrate.

03-09-06, 19:19	#23 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	yep...uncased rye. new pics coming in a few minutes.....it's much larger now...and a couple of new pins formed....

03-09-06, 22:05	#25 (permalink)
Jovy Mycophiliac Join Date: Jun 2005 Posts: 55	arent all those other lil brown spots pins also? G'luck __________________ dew that lay gone up face of wilderness lay round thing small as hoar frost ground children of Israel said one another manna they wist not what was Moses said unto This bread Lord given you to eat

03-10-06, 06:15	#26 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	no joyvy....if you look at the larger version of the picture...those are rye grains. Some of the grains in the second set of pics under the new pin are grains that are right up against the glass so the mycellium keeps getting banged into the glass when i pick itup....the "cake" of grains shrunk so much...there's now a 1/2" gap between the middle of the cake and the glass...at the top it's probably closer to a full inch....at the bottom...it's still regular size. Now for the question.....what to do with these? the grain that is...i was thinking of using it to spawn to some straw after i harvest the couple of mushies it has on it.

03-10-06, 11:09	#27 (permalink)
doodaa man Eating @ da Y VIP style Join Date: Nov 2005 Posts: 371	Green grain Watch out ! is that green i see on some of the grain ?

03-10-06, 18:02	#28 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	nope...it's blue. Some of the grains are VERY blue.

03-10-06, 18:55	#31 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	oh...this is all the same rye "cake"

03-10-06, 19:12	#32 (permalink)
the_chosen_one Darth Moderator Join Date: May 2005 Posts: 5,120	Holy shit!!! Clone it. Breed it. And certainly PM me for a trade when you are ready! __________________ "Luck favors the observant." - Workman

03-10-06, 19:36	#33 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	lol...yeah...all those little pins sprung up overnight. I'm curious as to how big this one's gong to get....contest anyone?

03-10-06, 21:40	#34 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	I agree with TCO, dude. Clone that sucker and use it as a culture source. How long has it been since you inoculated that jar? __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

03-10-06, 21:47	#35 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	innoculated on 1/6

03-10-06, 22:17	#37 (permalink)
the_chosen_one Darth Moderator Join Date: May 2005 Posts: 5,120	That's pretty old. No wonder its fruiting. My hat is off to you tho. That jar was definitely sterile! Musta had a high water content to slow it down that much and then fruit like that. When did the jar fill in? I would say let it finish invitro just to see how big they get. You can always clone it after harvest. I'd still clone it simply cuz it's one tuff son of b!!! __________________ "Luck favors the observant." - Workman

03-11-06, 10:25	#38 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	yeah...the jar was taking up space in my incubator and the other 4 qt's contammed with wet spot. These filled in in about 14 days and just sat there. Took them out and put them on my desk..kind of forgot about them. it's been cold in the house...it started to warm up the past week or so..today it's supposed to get up to 66oF a swing from a balmy 20 of last week. but i always keep it above 55 in the apartment. guess they liked the swing in temp. it's now got 4 little pins and the one big guy...which is growing real fast! if it keeps up this rate it will be hitting the top of the jar in a couple of days.

03-11-06, 10:27	#39 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	oh...and i found a single pin on the other jar.

03-11-06, 19:55	#40 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	The miracles of neglect-Tek! It's shocking how hearty these little guys are... __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

03-12-06, 10:31	#43 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	if you like invitro it'd be worth cloning a few of the bigger pins __________________ visit dial-a-hippie .

03-12-06, 10:36	#44 (permalink)
MurCurY I.Know.All Join Date: Oct 2005 Posts: 884	yeah...i thought of that. Now say i clone this big guy..or one of the smaller big guys... The clone would be genetically predisposed to fruit on grain?

03-12-06, 10:44	#45 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	exactly, to pin uncased __________________ visit dial-a-hippie .