freezing pasteurized doo for later use

[mycobri]

Ecuador strain, print to syringe
using the very first print ever traded here at mycotopia
thanks i_am_me
these spores were getting old and my friend has
never worked with EQ yet.

the only problem is that the furnace broke and was
off for a few weeks, leaving temps far too cold for cubies.
many other projects were lost

a heated FC was designed to keep temps in the mid 70's.
it is basically FC-in-tub
Attachment 80732
a short bin with a submersible fish tank heater on bottom
and a perlite FC up top.

also this is a total neglected chamber
meant for someone who is never home.
there are 6 quarter sized tyvek breathers
and an optional battery powered fan (which was not used yet)

This time No Fanning at all
and no casing either.

the sub was Donkey Doo and Straw mix from mycrotopia.
it had actually been in the freezer for several months.
it was left to thaw overnight
and then loaded into 4 quart jars and PC'd for 1 hour

4 1/2 pt. cakes were spawned to 1 gallon of D-DOO/Straw mix
and left to recover for 4 days

Attachment 80733
birthed to chamber
the EQ loves the Donkey doo and straw
even rhizos climbing up the plastic walls

after 6 days there were pinners
Attachment 80734
then a few more days and the ecuador spore stock is replenished
Attachment 80735
flush one harvest
Attachment 80736
Ecuador strain seems to be a medium sized cubensis
as this was the largest shroom from the first flush
Attachment 80737

a small chunk from a contaminated jar fruiting invitro
Attachment 80738Attachment 80739
a stray EQ cake
Attachment 80740

not the most amazing flush
and an un-even pinset,
but very happy that:
• the cold room temps were defeated
• more EQ spores to work with
• finally got to try the Donkey doo / straw mix from Mycrotopia

these small mushrooms were not picked yet
not sure if they are aborts or flush 2 coming up
Attachment 80741
anyways going to flood this sub and see what happens for flush two

Thanks for reading

[Hippie3]

far-out!

told folks it could be frozen for storage,
nice to see proof.

[hyphaenation]

Yeah ! A Mycobri thread.

Happy happy, joy joy

[klasamentes]

well done!

[stony_dude]

Coolfruieting chamber mang
stony

[mycobri]

thanks everyone
i'll be sure to post some more flushes

still have a little more donkey doo / straw in the freezer.
it thawed out nicely,
going to have to pick up some more

[dpwishy]

Did i read this right, you pced the poo for an hour after it thawed out? My buddy swim has a bunch of donkey doo in his freezer, wouldnt it be fine to just thaw it out and use it?

[mycobri]

i'm pretty sure you would have to re-pasteurize
after thawing out

these jars were PC'd which probably sterilized them,
which is not normally desired.
however it has worked well in several grows so far
so gave the PC another try

either way -good luck with your frozen mix

[Freaky]

This is a great thread! I like the fact it is thawable, if that's even a word?

[Hippie3]

i gotta disagree,
if frozen soon after pasteurization
no microbial growth should occur-
should be able to use as soon as thawed.
in theory.

[mycobri]

it works

even though i froze this batch after it sat out for about 5 days,
decided to put it to the test anyways.

the remaining frozen donkeydoo straw mix
was thawed out and spawned with no pasteurization.

the stray cake was spawned directly to the thawed mix

sure enough it seems to be growing fast and healthy

Attachment 81468Attachment 81469

[dpwishy]

My buddy swim just cased 6 gallons of donkey doo that came from the freezer tonight also, he has no doubt in his mind things will be fine. Good luck mycobri

love, peace, compassion and respect
-wishy

[eatyualive]

nice job!

[mycobri]

Thanks : ) this is another multispore, no casing, no fanning experiment,
print to small scale bulk

this bulk substrate mix has real potential
there would be some serious eye candy if a clone or perfect casing were applied

another flush began
but forgot to dunk
so cropped them early
and submerged the brick of EQ
Attachment 81754Attachment 81755
Attachment 81756Attachment 81757
there is a drain port and rubber stopper on the base of the tub

after a dunk - flush 2? fruited with breathing lid, outside of heated FC
Attachment 81758Attachment 81759
this flush seems better after the dunk.

i think this chamber loses rh fast when there is a shallow sub
(this sub is one gallon which is 1" deep in this tub)

hoping for a nice third flush..
i think the bottoms might have some fruiting power too
might flip the sub over or suspend it on a dishrack in the FC
any ideas welcome

[mycobri]

the un-pasteurized donkey-doo/straw tray
is now pinning
Attachment 82144
so here is the proof it can be used straight from the freezer
this batch was even in room temps for about 5 days before freezing
although that long was probably risking it



as far as the sub in the heated FC
it's headed towards a fourth flush
and is finally putting out larger fruits
Attachment 82145
gonna unplug the heat now thats it's warm
and dunk again to try for some more fruiting action

[Mermaidia]

Nice bri

EQ's are pretty shrooms.

for pins

[tex]

Awsome, Ive been wantin to move up to the to doo since ive gotten the cakes down pretty much and I have plenty of them to spare as spawn!

Thanks!!


Hippie....Maybe any special coming up on your doo/straw mix?

[omentheduck]

wow

[mycobri]

thanks

was very careful and removed the
donkey-doo/straw colonized block from its tub
and propped it up on a dishrack
Attachment 82669Attachment 82670
after these caps open
gonna dunk and go for one more flush
hoping to see fruiting from all sides

[mycobri]

what a difference a day can make
Attachment 82739
the biggest one from flush four
Attachment 82740

definitely going to order more Donkey-doo and straw

[Freaky]

Great job Mycobri! 4 flushes and looks like it could give some more.

This is very informative, as now peeps can pasteurize, freeze, thaw and use when needed

[Hippie3]

yep.
just need more freezer space.