2nd round has begun...

Cheebabear · 09-21-06, 23:10

I whipped up 9 1/2pint PF jars the yesterday and pc'd em for 60minutes, today I innoculated them each at four points per jar with Mazatepec spores. I used about 1 ml per jar. One ml was injected into a liquid culture for future innoculations. Got all 9 jars and the liquid culture in a closed cooler with a submersible aquarium heater in a plastic bottle. Right now it's at 82oF with 80% RH. Now... I just wait.. and wait...wait..

This will be my second time cultivating mushrooms.. although the 1st time went on for a few months because I kept making popcorn & wbs spawn jars and liquid cultures for pf cakes. Got many cakes and flushes until I started getting many many contams, which thanks to this site I found out it was the Coco Coir I was using. I can't wait to see how this strain looks firsthand.. I'm going to make as many prints as I can this round to donate to the Topia.

Hippie, I have seen how you prefer spore prints. My first round cultivating, I sterilized a small stack of 3"x5" index cards in the PC and printed onto those, folded them in half and taped the edges, vacuum sealed them in a small plastic bag with small amount of dessicant. Am I asking for trouble using the index cards? This time around I will use foil as it seems to be the preferred method by most on here.

Will post pictures once I see some myc growth.

Hippie3 · 09-22-06, 07:19

paper prints often germinate prematurely once converted into syringes due to the nutrients of the tiny bits of paper scrapped loose

dial8 · 09-22-06, 09:12

I like foil. Try and get the heavy duty. It will make the printing process much easier, and when you scrape the spores off the foil will not tear as easily.

nomoreusmc · 09-22-06, 09:17

Quote:

Originally Posted by dial8

I like foil. Try and get the heavy duty. It will make the printing process much easier, and when you scrape the spores off the foil will not tear as easily.

It's all I use, and is quick easy and reliable.

Cheebabear · 09-22-06, 13:31

Thanks for the info. I never read about the paper pieces germinating spores, good to know. I will definitely use foil from now on, but I still got some waiting to do to get to that point. I only hope I can control myself from opening the cooler to check on the jars every hour as if something noticeable might have grown in that time..

Pixie Dust · 09-22-06, 13:35

When you puncture the holes in the lids, do you do it before pc'ing and adding subtrate? or after? And how much do you innoculate into a pint jar? sorry if this is off topic ..

motograter · 09-22-06, 13:52

Quote:

Originally Posted by Pixie Dust

When you puncture the holes in the lids, do you do it before pc'ing and adding subtrate? or after? And how much do you innoculate into a pint jar? sorry if this is off topic ..

Yes, makethe holes in the lids before you even set up the substrate.

What I do is make the holes with a nail or ice pick. then add some silicone to each hole, this make a self healing site which make less chance of contams. You can pick up silicone at any auto parts store for a couple $$. Let the silicone set for a day or so, then mix up the substrate and PC it, and you're ready to go.

For a 1 pint, all you need is 1cc of spore solution.

Cheebabear · 09-22-06, 13:57

Quote:

Originally Posted by Pixie Dust

When you puncture the holes in the lids, do you do it before pc'ing and adding subtrate? or after? And how much do you innoculate into a pint jar? sorry if this is off topic ..

Well, the first time I used inverted metal canning lids and poked the holes before PC'ing, but I covered them with tape and/or foil before PCing. After reading browsing the Topia some more, I've seen a lot of people talk against using tape. I had also tried a single hole in the middle of an inverted metal canning lid stuffed with polyfill, and again this was all done before PCing and covered with foil.

This time I used Tyvek to cover the jars and covered with foil, then PCed them and poked holes while innoculating.

So if you are going to punch holes in a lid, I would do it before PCing. It doesn't matter if its before or after adding substrate as long as you PC it afterwards. As far as how much to inocculate per jar, I personally try to use 1ml per jar in 4 different innoculation points (.25 ml per innoculation site).

Pixie Dust · 09-22-06, 15:40

Thanks guys, Also one more thing, when Innoculating, Is it BEST to do it in a glove box if you want a few flushes
, or doing it in a closed room after disinfecting it when it comes to growth rate and fruiting?

(what is your best personal opinion to what makes more fruiting? Subtrate mix, inncubator, D.E.C, Dunk, etc?)

motograter · 09-22-06, 17:04

You can do it open air. but make sure you flame/alch. wipe the needle tip after EACH jar.

Cheebabear · 09-22-06, 17:58

Quote:

Originally Posted by Pixie Dust

Thanks guys, Also one more thing, when Innoculating, Is it BEST to do it in a glove box if you want a few flushes
, or doing it in a closed room after disinfecting it

I have never used a glove box as of yet. I make sure the AC and any fans are off, spray the air with Ozium or other air disinfectant, and wipe down kitchen table with disinfectant. I also wear a surgical type mask, hairnet, and latex gloves while innoculating. Be sure to follow motograters advice about flaming and wiping with alcohol.

Quote:

Originally Posted by Pixie Dust

when it comes to growth rate and fruiting?

(what is your best personal opinion to what makes more fruiting? Subtrate mix, inncubator, D.E.C, Dunk, etc?)

I really don't know how to answer this. So many things together can affect growth rate and fruiting. Good temp,humidity, and air exchange I guess would be my main focus.

[/quote]

Cheebabear · 09-26-06, 20:03

We have colonization.... here are some pictures of a couple of the jars along with the incubator. The spore syringe used to innoculate these had been stored in a box in the fridge for quite some time, so I was worried if it they would still be as viable, but they appear to be doing fine so far.

09-26-06, 20:29

lookin' strong bro..

Cheebabear · 10-27-06, 18:15

Just to update. It was 9/21/06 when the jars were innoculated, it's now been a little over a month and no pins and slow colonization on the bottom of the jars. I flipped the jars over and they're slowing filling in. A couple of jars have started to shrink a bit but still no pinning on any jars but you can still see the rope like mycelial growth on many jars.

Temp and humidity is fine. I"m thinking the BR I used may be the reason for slow growth, or the amount/lack of moisture in the substrate. I had pretty slow growth my first grow too but once they started popping they were good and continued for a while. No contams that I have seen so far.

Hopefully won't be much longer... got to get the fruiting chamber set back up anyway.

spacecake · 10-27-06, 19:10

Good luck cheebabear !!!
Your on the right track...

Hey hippie ..you are looking good today,.. beauty comes with age.. not !!!

Hippie3 · 10-27-06, 21:12

are you exposing to light daily ?
should immediately if not.

Hippie3 · 10-27-06, 21:12

Quote:

Originally Posted by spacecake

Hey hippie ..you are looking good today,.. beauty comes with age.. not !!!

it's a good thing that one's eyesight declines even faster

Cheebabear · 10-27-06, 22:08

Quote:

Originally Posted by Hippie3

are you exposing to light daily ?
should immediately if not.

I haven't been, no. I will begin doing so immediately. How much exposure should they receive? I wouldn't want to expose them too little or too much light.

Thanks

gunji · 10-28-06, 15:10

Injection sites did you not? that will slow or stall your jars, they need to have some gas exchange, i would suggest removeing at least 2 blobs of silicone from each jar, then turn the jars upside down, i stack mine up 4 high that way, and rotate the bottom one to the top each day so they all get to breath properly.

I keep my jars at about the same enviroment as yours and have full colonization in 10 to 14 days.

i used the pf tek max fruiting formula with just a drop or 2 of liquid seaweed in the water per jar

the dry verm layer acts as a barrier to contams , everyone here always says remove tape let em breath etc so its what i do, it works, im just a newb myself passing on what i read in this forums

Gunji

Cheebabear · 10-28-06, 19:55

Quote:

Originally Posted by gunji

Injection sites did you not? that will slow or stall your jars, they need to have some gas exchange, i would suggest removeing at least 2 blobs of silicone from each jar, then turn the jars upside down, i stack mine up 4 high that way, and rotate the bottom one to the top each day so they all get to breath properly.

No. The tops of all my jars are tyvek only which provides plenty of gas exchange. In the last pic I posted the jars were all turned upside down so I could show the lack of colonization on some of the bottoms.

I did not use the "max fruiting formula", just a straight BRF,vermiculite,water mix. I could never find true BRF and I bought brown rice and ground it up in a coffee grinder. Plus the bag of self made BRF has been in the pantry for a few months (since my first grow). Overall I think that's the main reason for the slow growth. I really must try a better formula. I just have a hard time finding all the ingredients.

Good luck on your grow! Is it your first? What strain are you using?

gunji · 10-28-06, 20:12

Ive had fruits from my first grow, was mexi-cub john allen and ban thuan over all the mexi-cub was first to fruit the john allens were the fattest, and the bt's the prettiest.

im working with some chitwan's and some penis envy uncut, the penis envy strain is by far the most agressive fastest colonizer so far that ive seen. from Sporeworks btw and they hooked me up with a freebie of chitwan too , very cool guys

ive noticed all my cakes are dry (lightweight) when i birth them even the ones that colonize in 10 days you might try injecting 10cc,s of sterile water into one of your ckes, trun it right side up and observe it for a couple days, that would tell you if they have dried out
i did that with stalled pint jar pf style that i had awhile back and it helped

cap · 11-04-06, 11:17

Quote:

Originally Posted by gunji

Ive had fruits from my first grow, was mexi-cub john allen and ban thuan over all the mexi-cub was first to fruit the john allens were the fattest, and the bt's the prettiest.
im working with some chitwan's and some penis envy uncut, the penis envy strain is by far the most agressive fastest colonizer so far that ive seen. from Sporeworks btw and they hooked me up with a freebie of chitwan too , very cool guys
ive noticed all my cakes are dry (lightweight) when i birth them even the ones that colonize in 10 days you might try injecting 10cc,s of sterile water into one of your ckes, trun it right side up and observe it for a couple days, that would tell you if they have dried out
i did that with stalled pint jar pf style that i had awhile back and it helped

heya

what was the outcome with the PE Uncut? from multispore?
were u able to take any pics? itd be super sweet to see em in action
ive yet to see a grow of them other than Workman's photos (which are wild!)
i wonder how they print

Cheebabear · 11-07-06, 11:37

Well, I started getting pins on most of the jars so I went ahead and birthed them all. Not a very good flush. I'm hoping after this flush that dunking them will improve the next flush.

Cheebabear · 11-15-06, 13:53

I had a meager first yield. I dunked cakes for 24hours and then double end cased them with peat moss and put them in the terrarium. There is new myc. growth showing through. Hopefully the fruits will be more plentiful this next time.

spacecake · 11-15-06, 14:41

In my experiances..a bad first flush will result in a very good second flush after a dunk and a d.e. casing.
The cake has still most of it nutriences after the first so it will prob do better in the second ..so no worries !

Cheebabear · 01-03-07, 14:54

Well, just to update on the Mazatepecs. The 2nd flush did turn out better than the first but still fell short of what I was hoping for from 9 cakes. I had a lot of aborts and eventually I scraped the 9 cakes clean and dunked them using the bleach tek. Only this time after dunking I broke up 3 cakes at a time and put them into a tub with the bottoms blacked out and cased them with peat, so that I ended up with 3 tubs. I put them in a cooler with a heat bomb to keep temps near 78 - 81oF but so far I can't see any myc poking through the casing layer. Here are some pics of the total yield from 2 flushes and the current casings. I was able to get a couple of good spore prints and I ordered some of Hippie's premium substrate mix. It is a lot easier buying it from him than it is trying to find all those ingredients, and it's not a bad price either. The ground up brown rice I used is pretty old and I think that may have had something to do with the grow, that and/or because the spores were fairly old although they were kept in the dark in the fridge.

Cheebabear · 01-05-07, 14:15

Quote:

Originally Posted by Cheebabear

Well, just to update on the Mazatepecs. The 2nd flush did turn out better than the first but still fell short of what I was hoping for from 9 cakes. I had a lot of aborts and eventually I scraped the 9 cakes clean and dunked them using the bleach tek. Only this time after dunking I broke up 3 cakes at a time and put them into a tub with the bottoms blacked out and cased them with peat, so that I ended up with 3 tubs. I put them in a cooler with a heat bomb to keep temps near 78 - 81oF but so far I can't see any myc poking through the casing layer. Here are some pics of the total yield from 2 flushes and the current casings. I was able to get a couple of good spore prints and I ordered some of Hippie's premium substrate mix. It is a lot easier buying it from him than it is trying to find all those ingredients, and it's not a bad price either. The ground up brown rice I used is pretty old and I think that may have had something to do with the grow, that and/or because the spores were fairly old although they were kept in the dark in the fridge.

Whoops. Here are the pictures that were supposed to have been in the previous post. One of total yield from two flushes of 9 BRF Cakes and the current tubs waiting for the myc to poke through:

Cheebabear · 02-02-07, 14:01

The three tubs were bunk. They just never took off, but I think the cakes were weak to beging with as they were the ones mentioned above that had a meager 1st flush. I was able to get enough spore prints to start some lc's. I made a syringe from a spore print and started some popcorn. Everything is looking good at this point.

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09-21-06, 23:10	#1 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	2nd round has begun... I whipped up 9 1/2pint PF jars the yesterday and pc'd em for 60minutes, today I innoculated them each at four points per jar with Mazatepec spores. I used about 1 ml per jar. One ml was injected into a liquid culture for future innoculations. Got all 9 jars and the liquid culture in a closed cooler with a submersible aquarium heater in a plastic bottle. Right now it's at 82oF with 80% RH. Now... I just wait.. and wait...wait.. This will be my second time cultivating mushrooms.. although the 1st time went on for a few months because I kept making popcorn & wbs spawn jars and liquid cultures for pf cakes. Got many cakes and flushes until I started getting many many contams, which thanks to this site I found out it was the Coco Coir I was using. I can't wait to see how this strain looks firsthand.. I'm going to make as many prints as I can this round to donate to the Topia. Hippie, I have seen how you prefer spore prints. My first round cultivating, I sterilized a small stack of 3"x5" index cards in the PC and printed onto those, folded them in half and taped the edges, vacuum sealed them in a small plastic bag with small amount of dessicant. Am I asking for trouble using the index cards? This time around I will use foil as it seems to be the preferred method by most on here. Will post pictures once I see some myc growth. __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

09-22-06, 07:19	#2 (permalink)
Hippie3 Admin Join Date: Feb 2001 Posts: 36,326	paper prints often germinate prematurely once converted into syringes due to the nutrients of the tiny bits of paper scrapped loose __________________ GROW SUPPLIES: www.Mycrotopia.com ------------Simply The Best------------ Namaste Temet Nosce

09-22-06, 13:31	#5 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	Thanks for the info. I never read about the paper pieces germinating spores, good to know. I will definitely use foil from now on, but I still got some waiting to do to get to that point. I only hope I can control myself from opening the cooler to check on the jars every hour as if something noticeable might have grown in that time.. __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

09-26-06, 20:03	#12 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	...and they're off! We have colonization.... here are some pictures of a couple of the jars along with the incubator. The spore syringe used to innoculate these had been stored in a box in the fridge for quite some time, so I was worried if it they would still be as viable, but they appear to be doing fine so far. Attached Thumbnails __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

09-22-06, 09:12	#3 (permalink)
dial8 Un Undead ***er8tor Join Date: Jun 1976 Posts: 3,185	I like foil. Try and get the heavy duty. It will make the printing process much easier, and when you scrape the spores off the foil will not tear as easily.

09-22-06, 13:35	#6 (permalink)
Pixie Dust Mycophage Join Date: Sep 2006 Posts: 122	When you puncture the holes in the lids, do you do it before pc'ing and adding subtrate? or after? And how much do you innoculate into a pint jar? sorry if this is off topic ..

09-22-06, 15:40	#9 (permalink)
Pixie Dust Mycophage Join Date: Sep 2006 Posts: 122	Thanks guys, Also one more thing, when Innoculating, Is it BEST to do it in a glove box if you want a few flushes , or doing it in a closed room after disinfecting it when it comes to growth rate and fruiting? (what is your best personal opinion to what makes more fruiting? Subtrate mix, inncubator, D.E.C, Dunk, etc?)

09-22-06, 17:04	#10 (permalink)
motograter Mycotopiate Join Date: Dec 2005 Posts: 627	You can do it open air. but make sure you flame/alch. wipe the needle tip after EACH jar.

09-26-06, 20:29	#13 (permalink)
~CoyoteMesc Guest Posts: n/a	lookin' strong bro..

10-27-06, 18:15	#14 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	..slow going but going Just to update. It was 9/21/06 when the jars were innoculated, it's now been a little over a month and no pins and slow colonization on the bottom of the jars. I flipped the jars over and they're slowing filling in. A couple of jars have started to shrink a bit but still no pinning on any jars but you can still see the rope like mycelial growth on many jars. Temp and humidity is fine. I"m thinking the BR I used may be the reason for slow growth, or the amount/lack of moisture in the substrate. I had pretty slow growth my first grow too but once they started popping they were good and continued for a while. No contams that I have seen so far. Hopefully won't be much longer... got to get the fruiting chamber set back up anyway. Attached Thumbnails __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

10-27-06, 19:10	#15 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 3,806	Good luck cheebabear !!! Your on the right track... Hey hippie ..you are looking good today,.. beauty comes with age.. not !!! __________________ Neurologic

10-27-06, 21:12	#16 (permalink)
Hippie3 Admin Join Date: Feb 2001 Posts: 36,326	are you exposing to light daily ? should immediately if not. __________________ GROW SUPPLIES: www.Mycrotopia.com ------------Simply The Best------------ Namaste Temet Nosce

10-28-06, 15:10	#19 (permalink)
gunji Mycophiliac Join Date: Sep 2006 Posts: 86	you used silicone self healing Injection sites did you not? that will slow or stall your jars, they need to have some gas exchange, i would suggest removeing at least 2 blobs of silicone from each jar, then turn the jars upside down, i stack mine up 4 high that way, and rotate the bottom one to the top each day so they all get to breath properly. I keep my jars at about the same enviroment as yours and have full colonization in 10 to 14 days. i used the pf tek max fruiting formula with just a drop or 2 of liquid seaweed in the water per jar the dry verm layer acts as a barrier to contams , everyone here always says remove tape let em breath etc so its what i do, it works, im just a newb myself passing on what i read in this forums Gunji

10-28-06, 20:12	#21 (permalink)
gunji Mycophiliac Join Date: Sep 2006 Posts: 86	Ive had fruits from my first grow, was mexi-cub john allen and ban thuan over all the mexi-cub was first to fruit the john allens were the fattest, and the bt's the prettiest. im working with some chitwan's and some penis envy uncut, the penis envy strain is by far the most agressive fastest colonizer so far that ive seen. from Sporeworks btw and they hooked me up with a freebie of chitwan too , very cool guys ive noticed all my cakes are dry (lightweight) when i birth them even the ones that colonize in 10 days you might try injecting 10cc,s of sterile water into one of your ckes, trun it right side up and observe it for a couple days, that would tell you if they have dried out i did that with stalled pint jar pf style that i had awhile back and it helped

11-07-06, 11:37	#23 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	Update Well, I started getting pins on most of the jars so I went ahead and birthed them all. Not a very good flush. I'm hoping after this flush that dunking them will improve the next flush. Attached Thumbnails __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

11-15-06, 13:53	#24 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	I had a meager first yield. I dunked cakes for 24hours and then double end cased them with peat moss and put them in the terrarium. There is new myc. growth showing through. Hopefully the fruits will be more plentiful this next time. Attached Thumbnails __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

11-15-06, 14:41	#25 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 3,806	In my experiances..a bad first flush will result in a very good second flush after a dunk and a d.e. casing. The cake has still most of it nutriences after the first so it will prob do better in the second ..so no worries ! __________________ Neurologic

01-03-07, 14:54	#26 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	Well, just to update on the Mazatepecs. The 2nd flush did turn out better than the first but still fell short of what I was hoping for from 9 cakes. I had a lot of aborts and eventually I scraped the 9 cakes clean and dunked them using the bleach tek. Only this time after dunking I broke up 3 cakes at a time and put them into a tub with the bottoms blacked out and cased them with peat, so that I ended up with 3 tubs. I put them in a cooler with a heat bomb to keep temps near 78 - 81oF but so far I can't see any myc poking through the casing layer. Here are some pics of the total yield from 2 flushes and the current casings. I was able to get a couple of good spore prints and I ordered some of Hippie's premium substrate mix. It is a lot easier buying it from him than it is trying to find all those ingredients, and it's not a bad price either. The ground up brown rice I used is pretty old and I think that may have had something to do with the grow, that and/or because the spores were fairly old although they were kept in the dark in the fridge. __________________ "...Born to bear and bring to all the details of our ending, to write them down for all the world to see...but I forgot my pen..."

02-02-07, 14:01	#28 (permalink)
Cheebabear Mycotopiate Join Date: Jan 2006 Posts: 264	Update... The three tubs were bunk. They just never took off, but I think the cakes were weak to beging with as they were the ones mentioned above that had a meager 1st flush. I was able to get enough spore prints to start some lc's. I made a syringe from a spore print and started some popcorn. Everything is looking good at this point.