Invitro Question

whiteshadow · 12-06-06, 18:55

I am trying the invitro method with 1/2 pints, once fully colonized do I:

1)remove dry verm once colonized?
2)flip the jars over once colonized? (I have waterproof tape on the innoc holes)
3)do I open them up to breathe at all after colonization?

Soliver1 · 12-06-06, 19:31

Flip 'em over (top side down) and do nothing else.

Whatever you do, DON'T open those jars, or you run a high risk of contaminating the jars.... Honestly, you don't even have to flip the jars, but I think it's an accepted practice, something about CO2 draining out...

soliver

the jesus · 12-06-06, 19:35

You should really take the tape off the holes, air exchange is critical. The dry verm barrier on top works as a filter.

1. Yes once the cake is fully colonized you can open the jar and remove the dry verm to make more room.

2. After you remove the dry verm you should flip the jars and loosen the lid a bit.

3. Once you are in the fruiting stage you can unscrew the top and air them out a few times a day.

Good luck bro.

whiteshadow · 12-06-06, 20:27

OK, now im confused, one person says yes, other says no...

Lazlo · 12-06-06, 20:39

This is how I do mine. I give the jars as much light as I can from the get go. Once the jars are fully colonized, I take off the lids and water the vermiculite barrier to the point it won't hold any more water. Usually there's pins already forming by this point if you've provided enough light from the time they were inoculated. Then put the lids back on and simply allow the jars to fill up with mushrooms. Watering the barrier gives the cakes more moisture for fruiting. Just make sure they are 100% colonized before opening the jars and watering the barrier.

the jesus · 12-06-06, 21:33

I am thinking that Soliver must not have realized that you were asking about AFTER 100% colonization, because it is safe once they are colonized and I am sure he knows that.

Once again best of luck

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12-06-06, 18:55	#1 (permalink)
whiteshadow Mycophiliac Join Date: Nov 2006 Posts: 21	Invitro Question I am trying the invitro method with 1/2 pints, once fully colonized do I: 1)remove dry verm once colonized? 2)flip the jars over once colonized? (I have waterproof tape on the innoc holes) 3)do I open them up to breathe at all after colonization?

12-06-06, 20:39	#5 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,692	This is how I do mine. I give the jars as much light as I can from the get go. Once the jars are fully colonized, I take off the lids and water the vermiculite barrier to the point it won't hold any more water. Usually there's pins already forming by this point if you've provided enough light from the time they were inoculated. Then put the lids back on and simply allow the jars to fill up with mushrooms. Watering the barrier gives the cakes more moisture for fruiting. Just make sure they are 100% colonized before opening the jars and watering the barrier. __________________ How can you have any pudding, if you don't eat your MEAT?

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12-06-06, 19:31	#2 (permalink)
Soliver1 Deranged Join Date: Feb 2005 Posts: 637	Flip 'em over (top side down) and do nothing else. Whatever you do, DON'T open those jars, or you run a high risk of contaminating the jars.... Honestly, you don't even have to flip the jars, but I think it's an accepted practice, something about CO2 draining out... soliver

12-06-06, 19:35	#3 (permalink)
the jesus mayor of boozeville Join Date: Mar 2006 Posts: 2,637	You should really take the tape off the holes, air exchange is critical. The dry verm barrier on top works as a filter. 1. Yes once the cake is fully colonized you can open the jar and remove the dry verm to make more room. 2. After you remove the dry verm you should flip the jars and loosen the lid a bit. 3. Once you are in the fruiting stage you can unscrew the top and air them out a few times a day. Good luck bro.

12-06-06, 20:27	#4 (permalink)
whiteshadow Mycophiliac Join Date: Nov 2006 Posts: 21	OK, now im confused, one person says yes, other says no...

12-06-06, 21:33	#6 (permalink)
the jesus mayor of boozeville Join Date: Mar 2006 Posts: 2,637	I am thinking that Soliver must not have realized that you were asking about AFTER 100% colonization, because it is safe once they are colonized and I am sure he knows that. Once again best of luck