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Old 07-25-07, 12:57   #1 (permalink)
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Open air bag innoculation W/0 Inj. Port

Is it feasable (or should I say wise) to innoculate large grain bags in open air? I have a glove box but these bags are frigging huge and it can only hold 1 at a time so it is very time consuming. If I get it all ready to tape, shoot, begin taping and remove needle at the same time with a table top ionizer as well could one get away with this method? Or should I just make some ports?
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Old 07-25-07, 14:57   #2 (permalink)
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The way i do em' is to use a magic marker to make a dot in the spot that u want to shoot [the solvent in the pen sanitizes the plastic]..

Use alcohol, not heat to clean the needle and shoot thru the dot...
I put a dab of glue on the hole [goop] and let dry for a few hours b4 massaging the bag to mix..Can be done in open air but best to wear gloves.

The Ionizer should help in still air...Gluk...
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Old 07-25-07, 17:59   #3 (permalink)
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You are pretty smart Golly, I don't care what Hip says. J/K
That is a great idea, my problem was the tape was not sticking after swabing with alchahol so I had to use giant pieces of tape. Metal tape that costs 23 buck a roll. (It is awsome and can handle any thing your PC can throw at it). Anyway, it doesn't look so pretty, your idea is much more aesthetically pleasing I imagine. I save 1 bag to try something else. Gonna use your tip, my fiend. Thanks again!
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Old 07-25-07, 18:20   #4 (permalink)
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If you can't do it in a glovebox, do it in a small room like a bathroom with absolutely no air movement at all. (turn of your AC or heat)

Spray a good amount of Oust in the air before innoculation.
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Old 07-25-07, 19:05   #5 (permalink)
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Quote:
Originally Posted by reefer View Post
If you can't do it in a glovebox, do it in a small room like a bathroom with absolutely no air movement at all. (turn of your AC or heat)

Spray a good amount of Oust in the air before innoculation.
I never would inoculate anything without a still room that had been fully Ousted, a glovebox, Lysol or Alcohol, gloves, tyvek sleeves, and a face mask.

Open air seems to be begging for problems.

What's this about an Ionizer? I have one. Are they effective in knocking out contams when inoculating? How do you use it?
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Old 07-25-07, 19:08   #6 (permalink)
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Why don't you just dab some silicone caulking on the bag and that will be a self healing port.?
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Old 07-25-07, 19:10   #7 (permalink)
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Quote:
Originally Posted by brodus View Post
I never would inoculate anything without a still room that had been fully Ousted, a glovebox, Lysol or Alcohol, gloves, tyvek sleeves, and a face mask.

Open air seems to be begging for problems.

What's this about an Ionizer? I have one. Are they effective in knocking out contams when inoculating? How do you use it?
I wear surgical mask, shower cap, use oust (the unscented rules), and of course all the other things (accept tyvek sleeves, I disinfect to the elbows and constanly re do my gloves and arms). I also have a truehepa filter (honeywell brand for a bigger room than mine.) I let the filter run, outs, let it run. Then turn it off and use the aspen air. Just when you inject 60-120 cc the needle is in there for awhile (sometimes twice) so I was thinking maybe making Inj. ports is worth while. My glovebox is too small.
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Old 07-25-07, 19:11   #8 (permalink)
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Quote:
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Why don't you just dab some silicone caulking on the bag and that will be a self healing port.?
After PCing? Won't the point of inj and the silicone harbor contams?

That is the only reason I did not make one after I PC'd them.

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Old 07-25-07, 19:47   #9 (permalink)
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I inoculate my Mycrotopia mycromix bags open air and it works great.

Golly, thanks for the glue tip
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Old 07-25-07, 21:38   #10 (permalink)
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I inoculate my Mycrotopia mycromix bags open air and it works great.

Golly, thanks for the glue tip

I dig your Avatar Freaky--do you have a link to that artist?
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Old 07-25-07, 21:44   #11 (permalink)
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Thanks Brodus!

No, sadly I don't know who the artist is, don't even remember where I found it on the net.
I fell in love with it right when I saw it and didn't pay attention to the finer details
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Old 06-23-08, 01:26   #12 (permalink)
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Nothing like digging up an old thread to make an off-topic post, right? Freaky's avatar is the company logo for Running With Scissors (http://www.runningwithscissors.com). They are the developers responsible for the Postal series video game - a game featuring over-the-top violent humor.
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Old 06-23-08, 11:49   #13 (permalink)
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Quote:
Originally Posted by sweeney View Post
Nothing like digging up an old thread to make an off-topic post, right? Freaky's avatar is the company logo for Running With Scissors (http://www.runningwithscissors.com). They are the developers responsible for the Postal series video game - a game featuring over-the-top violent humor.
Sweeney, as you said, an old post......Brodus was referring to my old Avatar.

Click my name here, and it is my profile pic:
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And just for an on topic post, Golly's glue trick after inoculating the bags works great!
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Old 06-23-08, 14:49   #14 (permalink)
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