i have got a good idea just need answers to my questions

bohner · 08-08-07, 17:08

Ok i will state my questions first then show you all what my plan is.

1) Does mycelium require Oxygen to grow?

2) After you inoculate your substrate with spores and the mycelium starts to grow does the mycelium produce any gases?

3) If so, any kind of gases that would stall it from growing? Ex:CO2.

I Know i can search for this one but I've already started this thread so,

4) Is CO2 heavier than oxygen, or can you displace CO2 with oxygen or just regular air?

5) Can you buy 3-7 Inch Needles for syringes?

Ok my explanation.

If my Questions are answered to what i hope they will be i am hoping to use the aquarium pump to pump in fresh air into many jars to remove any bad gases and and replace with good gases. I have been using this basic method to bubble my LC which seems to speed up the myceliums growth to a point but no matter what, i can only get it to grow so far in the LC with or without the bubbling of it so then i transfer it to the substrate. By using the polyfil in both the syringes you reduce the chances for contaminations and you equalize the pressure as to not blow up the jar. I have been bubbling my LC's for awhile and have never had a contam yet.
So does anyone have any Questions for me or any tips.

~Hippie3 · 08-08-07, 17:23

pretty much YES to all those ?s.
just regular air is fine, needed too.

next step to better lc is magnetic stir bar .

CoyoteMesc · 08-08-07, 17:38

Good luck bohner

bohner · 08-08-07, 18:04

well would it be better to have a long needle that would reach the bottom of the substrate and feed the mycelium and try to push the CO2 out through the other syringe?

~Hippie3 · 08-08-07, 18:24

you gave us this thread-
http://forums.mycotopia.net/liquid-c...rt-action.html (Bubbling your LC: airport in action)
so you know the deal.
as long as your needle is bubbling thru the liquid
doesn't much matter if it's longer.
you want dramatically better growth
add a stir plate and stir bars.

bohner · 08-08-07, 18:30

ha ha sorry i should have made it more clear this is not for LC it is for using in say a rye substrate. i am just wondering if i add more air or basically help exchange the air in the substrate more efficiently will the mycelium colonize faster?

again i am sorry for not being more clear.

~Hippie3 · 08-08-07, 18:33

only if the extra air is properly humidified first
can you get by with that,
the flow of air will dry the grains quickly.
proly best not to attempt
as only a very minimal amount of air exchange is needed,
more will not improve growth/yield, etc.

bohner · 08-08-07, 18:41

haha so the new name of the thread should be a bad idea

Quote:

Originally Posted by Hippie3

the flow of air will dry the grains quickly

i never thought about that.

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08-08-07, 17:08	#1 (permalink)
bohner Mycophiliac Join Date: Oct 1972 Posts: 62	i have got a good idea just need answers to my questions Ok i will state my questions first then show you all what my plan is. 1) Does mycelium require Oxygen to grow? 2) After you inoculate your substrate with spores and the mycelium starts to grow does the mycelium produce any gases? 3) If so, any kind of gases that would stall it from growing? Ex:CO2. I Know i can search for this one but I've already started this thread so, 4) Is CO2 heavier than oxygen, or can you displace CO2 with oxygen or just regular air? 5) Can you buy 3-7 Inch Needles for syringes? Ok my explanation. If my Questions are answered to what i hope they will be i am hoping to use the aquarium pump to pump in fresh air into many jars to remove any bad gases and and replace with good gases. I have been using this basic method to bubble my LC which seems to speed up the myceliums growth to a point but no matter what, i can only get it to grow so far in the LC with or without the bubbling of it so then i transfer it to the substrate. By using the polyfil in both the syringes you reduce the chances for contaminations and you equalize the pressure as to not blow up the jar. I have been bubbling my LC's for awhile and have never had a contam yet. So does anyone have any Questions for me or any tips. Attached Thumbnails

08-08-07, 17:38	#3 (permalink)
CoyoteMesc howling mad Join Date: Jan 2007 Posts: 4,087	Good luck bohner __________________ WOODY: Hey, Mr. Peterson, there's a cold one waiting for you. NORM: I know. If she calls, I'm not here.[ (Cheers)

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08-08-07, 17:23	#2 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	pretty much YES to all those ?s. just regular air is fine, needed too. next step to better lc is magnetic stir bar .

08-08-07, 18:04	#4 (permalink)
bohner Mycophiliac Join Date: Oct 1972 Posts: 62	well would it be better to have a long needle that would reach the bottom of the substrate and feed the mycelium and try to push the CO2 out through the other syringe?

08-08-07, 18:24	#5 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	you gave us this thread- http://forums.mycotopia.net/liquid-c...rt-action.html (Bubbling your LC: airport in action) so you know the deal. as long as your needle is bubbling thru the liquid doesn't much matter if it's longer. you want dramatically better growth add a stir plate and stir bars.

08-08-07, 18:30	#6 (permalink)
bohner Mycophiliac Join Date: Oct 1972 Posts: 62	ha ha sorry i should have made it more clear this is not for LC it is for using in say a rye substrate. i am just wondering if i add more air or basically help exchange the air in the substrate more efficiently will the mycelium colonize faster? again i am sorry for not being more clear.

08-08-07, 18:33	#7 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	only if the extra air is properly humidified first can you get by with that, the flow of air will dry the grains quickly. proly best not to attempt as only a very minimal amount of air exchange is needed, more will not improve growth/yield, etc.