Agar

WHITE RABBIT · 11-14-07, 20:28

I have agar, and potatoes.
I can't get malt or dextrose at the moment.
Can I use potato water and agar only?

Dr_T · 11-14-07, 20:36

Probably better to use it. But it might work.

Can't get dextrose at all? They sell tablets of it at Walgreens. Or swipe a couple of packs of "Sugar in the Raw" from Starbucks, and try that maybe.

If it works without, let us all know!

WHITE RABBIT · 11-14-07, 20:41

Didn't really know I can get it at walgreens.
I thought regular sugar was bad to use but I'm totally new at agar.
Thank you.

thebaldeagle · 11-15-07, 08:49

Malt is available at any beer/wine making shop, just use the light tan malt, labled as such. I read on here where someone was using light karo syrup instead of malt and it was working. They said it made nice clear plates. I don't know the ratio, but I'm sure someone will help you out.

yerbaadam · 11-15-07, 09:09

You can really use just about anything. But simple sugars like dextrose are easy to work with. Dextrose, which is usually derived from corn, is not much different from corn syrup. I have done plates with rice syrup (makes the plates a tint yellow), xylitol, agave, compost juice etc. Just about anything can be made as "food", stamets even talks about making dog food agar media. It is nice to have a variety of plates, with different nutrients, that way, as you transfer a culture several times, you can avoid senescence, and genetic degradation.
Good luck finding what you need. Check a good healthfood store if you haven't, or a brewery supply, even a micro brewery in yer area may be able to give you a hand.

>Yer friend,
NascAR denDriTE DUDley

~Hippie3 · 11-15-07, 09:10

i don't see why you couldn't get by ok
with using potato broth by itself
as the nutrient in the agar ,
also
options include adding a bit of nutritional yeast to the broth, and/or peptone .

thebaldeagle · 11-15-07, 09:59

I can't find peptone anyplace around here and I've been looking for years. I have since realized that since I don't store cultures for years I dont't "think" that I need it. I buy off the net, but only when I have to.

crazy1 · 11-15-07, 10:31

Peptone is a difficult thing to find. But you can also use a grain flour as the nitrogen source. Rye flour and rice flour have both done well for me. About 2 grams per 500ml
The nitrogen source isn't really for storage tbe, it's to help the myc run faster on the agar, or whatever sub you're using.
The Nut yeast as Hip said is also an awesome additive.
I use about 3 gramsms per 500 ml of agar

siam_jim · 11-15-07, 10:38

only heard of 1 guy using sweet potato and he swears it works great.... or use light karo..
you still can use just what you now w/o the dex...

siam

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11-14-07, 20:28	#1 (permalink)
WHITE RABBIT Let's take a trip Join Date: Mar 2007 Posts: 283	Agar I have agar, and potatoes. I can't get malt or dextrose at the moment. Can I use potato water and agar only? __________________ What? Who? Me?

11-14-07, 20:41	#3 (permalink)
WHITE RABBIT Let's take a trip Join Date: Mar 2007 Posts: 283	Didn't really know I can get it at walgreens. I thought regular sugar was bad to use but I'm totally new at agar. Thank you. __________________ What? Who? Me?

11-15-07, 10:31	#8 (permalink)
crazy1 VIP Member Join Date: Apr 2007 Posts: 874	Peptone is a difficult thing to find. But you can also use a grain flour as the nitrogen source. Rye flour and rice flour have both done well for me. About 2 grams per 500ml The nitrogen source isn't really for storage tbe, it's to help the myc run faster on the agar, or whatever sub you're using. The Nut yeast as Hip said is also an awesome additive. I use about 3 gramsms per 500 ml of agar __________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience

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11-14-07, 20:36	#2 (permalink)
Dr_T Random Dude Join Date: Aug 2007 Posts: 2,078	Probably better to use it. But it might work. Can't get dextrose at all? They sell tablets of it at Walgreens. Or swipe a couple of packs of "Sugar in the Raw" from Starbucks, and try that maybe. If it works without, let us all know!

11-15-07, 08:49	#4 (permalink)
thebaldeagle Mycophiliac Join Date: Sep 2007 Posts: 77	Malt is available at any beer/wine making shop, just use the light tan malt, labled as such. I read on here where someone was using light karo syrup instead of malt and it was working. They said it made nice clear plates. I don't know the ratio, but I'm sure someone will help you out.

11-15-07, 09:09	#5 (permalink)
yerbaadam SpectraL veNtriloQuist Join Date: Jul 1971 Posts: 798	You can really use just about anything. But simple sugars like dextrose are easy to work with. Dextrose, which is usually derived from corn, is not much different from corn syrup. I have done plates with rice syrup (makes the plates a tint yellow), xylitol, agave, compost juice etc. Just about anything can be made as "food", stamets even talks about making dog food agar media. It is nice to have a variety of plates, with different nutrients, that way, as you transfer a culture several times, you can avoid senescence, and genetic degradation. Good luck finding what you need. Check a good healthfood store if you haven't, or a brewery supply, even a micro brewery in yer area may be able to give you a hand. >Yer friend, NascAR denDriTE DUDley

11-15-07, 09:10	#6 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	i don't see why you couldn't get by ok with using potato broth by itself as the nutrient in the agar , also options include adding a bit of nutritional yeast to the broth, and/or peptone .

11-15-07, 09:59	#7 (permalink)
thebaldeagle Mycophiliac Join Date: Sep 2007 Posts: 77	I can't find peptone anyplace around here and I've been looking for years. I have since realized that since I don't store cultures for years I dont't "think" that I need it. I buy off the net, but only when I have to.

11-15-07, 10:38	#9 (permalink)
siam_jim Mycotopia Mod Join Date: Oct 2005 Posts: 1,853	only heard of 1 guy using sweet potato and he swears it works great.... or use light karo.. you still can use just what you now w/o the dex... siam

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