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Old 02-24-08, 15:49   #1 (permalink)
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Spawn Bag Tek

Greetings!

I've been lurking around this forum for quite some time and just placed my order for some spores on sporeworks!

Here's my situation.

I'm living in a 2 room apartment and want to grow steathily in my closet.

I can't afford a pressure cooker and have decided that a DIY spawn bag is the stealthiest route to get the MYC going before casing/fruiting in a backpack in my closet.

My plan is thus!

MYC Development

1. One turkey bag sterilized with isopropyl alcohol.
2. Fill with straw and verm.
3. Sterilize by shaking hydrogen peroxide and letting sit for several hours.
4. Rinse the substrate straw/verm substrate mixture in a collander.
5. Re-sterilize the bag with isopropyl and dump the mixture in.
6. Fasten a large O-Ring around the top of the turkey bag.
7. Tape sterilized Cheese-cloth over the top of the Opened bag.
8. Sterilize needle, knock dem' puppies up!
9. Clean the inside of backpack out with isopropyl wipe and Spray entirety with lysol.
10. Throw another bag around the turkey bag just in case of breakage.

Let sit until 70% colonized, then shake.

Fruiting

1. Cut the bottom out of one rubbermaid container.
2. Paint the bottom rubbermaid's insides black for stealth purposes. Wait to dry, and then sterilize with isopropyl.
3.Fill the other rubbermaid about 1/4 way up with hydro-peroxide sterilized straw.
4. Spray lysol around the area and break the bag open and mix the fully colonized bag created cake around in the straw with latex gloves on.
5. Mist with a 90/10 distilled water hydrogen peroxide solution.
6. Apply sterilized verm layer to the top.
7. Tape two simple white LED lights to the top of the case for fruiting light.
8. Wait. Harvest. I don't know if a dunk is possible, but i'll be sure to resaturate the SHIT out of it with that 90/10 misting solution.

Non Procedural Notes:

I'm planning on making two of these bags innoculated with 3cc's each just in case one fails. If both succeed, I'll just dump both in the the fruiting chamber anyway for the sake of faster colonization.

I am also planning on making a LC jar with the remaining 4cc's of spores just for the sake of having it.

I know there are plenty of guides here and I've read the karo guides/potato guides and all that jive. But if someone could point me in the right direction it'd be just spec-jiff-u-lar.

Even if the LC fails I'll be sure to take one of the fruits (hopefully! {:=} ) and use it to innoculate my next hopefully bulk scale grow when I get my own place!

This is my first time grow and any suggestions/constructive criticism would be greatly appreciated. Thanks!!!
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Old 02-24-08, 17:06   #2 (permalink)
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Ehhh, that sterilizing procedure will not work. You should've ordered Sporeworks' substrates as well. 5lbs. for around 25$ I believe.

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Old 02-24-08, 17:18   #3 (permalink)
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no pc means you gotta use steam, chemicals won't get it.
you'll need to mix brf up in bags and steam them 90 minutes,
not too much in a bag, just 2-3 cups per so steam can penetrate
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Old 02-24-08, 20:29   #4 (permalink)
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Old 02-25-08, 01:28   #5 (permalink)
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A nice spawn bag sounds just dandy, But I dont have any money for that right now and I want to get this project underway.

Isn't hydrogen peroxide a highly effective anti-bacterial?
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Old 02-25-08, 01:35   #6 (permalink)
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http://forums.mycotopia.net/fungi-gr...-bulk-tub.html

hope this might help
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Old 02-25-08, 02:41   #7 (permalink)
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thats an interesting article, but I'm trying to grow stealithly here haha!
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Old 02-25-08, 04:06   #8 (permalink)
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Sorry but your plan will not work. There are just many points for failure.
1st, the straw contains
peroxide-decomposing enzymes that will need to be killed first by steam or pressure-sterilization. Pasteurization of the straw is what you want to achieve anyhow. With sterile straw you will need to be completely sterile throughout every step in you grow process. Exposed uncolonized straw will contam quickly. You'll need to steam the bag in the very least as hip stated..

2nd, the Iso will kill off most of the spores when you inject them. Even if you do manage to get some spores to germinate, contaminates will have long since colonized your sub.

If you want stealth on the cheap then growing brf jars invitro is the way to go, but, you'll still need to at least steam the jars unless you buy them pre-sterilized. Since money is a major issue, you could and will have
better success if you load up your oven bag with some brf and verm and microwave the substrate. Done correctly you will have a completely chemical-free sterile substrate.
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Old 02-25-08, 09:10   #9 (permalink)
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Quote:
load up your oven bag with some BRF and verm and microwave the substrate .
or steam them at least 90 minutes in a pot with a snug lid.
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Old 02-25-08, 12:45   #10 (permalink)
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I don't mean to come off as arrogant.... But i'm positive that isopropyl alcohol evaporates relatively quickly so it's highly unlikely that the spores would die post sterilzation.

And what 'enzymes' are you talking about in the straw? I thought the purpose of the substrate was to provide a texturally/geometriccaly/nutritionally fit growing substrate for the mycalium.

And besides I can't be boiling a fuckin' pile of straw in my dads apartment kitchen for several hours haha...

And the bags... well due to the limited successes i've read and the 25$ price tag... i'd just rather not.
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Old 02-25-08, 12:55   #11 (permalink)
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What you propose will NOT work.

at home in Dads house sounds like a sure fire bust and a boot out of the home thing. LOL!!! Have Fun.
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Old 02-25-08, 12:56   #12 (permalink)
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Quote:
I don't mean to come off as arrogant.... But i'm positive that isopropyl alcohol evaporates relatively quickly so it's highly unlikely that the spores would die post sterilzation.

And what 'enzymes' are you talking about in the straw ? I thought the purpose of the substrate was to provide a texturally/geometriccaly/nutritionally fit growing substrate for the mycalium.

And besides I can't be boiling a fuckin' pile of straw in my dads apartment kitchen for several hours haha...

And the bags... well due to the limited successes i've read and the 25$ price tag... i'd just rather not.

Well that's a rude comment right there.
You ask for advice and you got some great info handed to you. Then you turn around and tell us what you are and aint gonna do. If you want 'specifics' use the search button at the top of the screen and find em yourself. Other wise, at least appreciate the help we are trying to give you.
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Old 02-25-08, 12:59   #13 (permalink)
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Another thing,

There are a great deal of members here with years of experience. Most can answer just about any cultivation question you can think of. Its YOUR job to ask the right questions.
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Old 08-07-08, 12:08   #14 (permalink)
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what a wanker !
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Old 08-07-08, 16:40   #15 (permalink)
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Lemme try to help you here. I'm a dad, so take it for what it's worth.

First. Move out. Get a real job. Pay your own bills. Then you can do whatever you want and stealth isn't a concern. That being said...

If you're looking for stealth, you're better off going with either a neglect tek, or chronic tek style grow, then using bags. If you're hellbent on doing this, (hey, i'm not your parent, I'm not going to tell you what you can/can't do) then try doing a couple PF-style cakes, and make LC with the rest of your multispore. Use the cakes as your substrate, case if you want, or don't, and just grow invitro or chronic. At the end of your experiment, you should end up with enough LC to further your education in mycology, and perhaps some fruits to eat with your friends (i'm assuming this is the main reason you wanna grow)

Second. Learn to tell the difference between people giving advice, and telling you what to do. I'm going to guess that 90-95% of the people who read/post here know more then you about mycology. I'm a pretty smart guy with a few different degrees from a couple different colleges, and I wouldn't even -think- about assuming that I know more about mycology then anyone here. If you ask a question, be thankful for the answer, and respectful to the answerer, regardless of what the answer is.

Now, let me stow away my soap box, and if you have any questions we'd be happy to help you out. Assuming you read and digest the above first.

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Old 08-07-08, 16:42   #16 (permalink)
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oh, one more thing. Coyote. your sig video is the funniest damn thing I've seen in a long, long time. You win the prize.
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Old 08-07-08, 17:41   #17 (permalink)
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Quote:
Originally Posted by diminishedlogic View Post
I don't mean to come off as arrogant.... But i'm positive that isopropyl alcohol evaporates relatively quickly so it's highly unlikely that the spores would die post sterilzation.

And what 'enzymes' are you talking about in the straw? I thought the purpose of the substrate was to provide a texturally/geometriccaly/nutritionally fit growing substrate for the mycalium.

And besides I can't be boiling a fuckin' pile of straw in my dads apartment kitchen for several hours haha...

And the bags... well due to the limited successes i've read and the 25$ price tag... i'd just rather not.
Hey, you know what? I don't mean to sound rude, but go for it. Use your idea. I've spent thousands of dollars on the learning process. If you're not old enough to be on your own, and you're wanting to grow some mushrooms, I'd say to just save your allowance, or however you have money, and that day when you can move out, BOOM, get it started. And in the meantime, read up and come up with a plan that will work.
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Old 08-09-08, 21:40   #18 (permalink)
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I must agree with everybody who has tried to help you out, you need to pasteurize your straw, rubbing alcohol is pointless for straw. You would probably end up killing the beneficial bacteria that pasteurization will allow to live. Get some water to boiling, take it to a convienent stealthy spot outside and add your straw, by this time it will be down to 180, put a cover on it and watch the temp, add more hot water to keep up the temp for about 2 or 3 hrs. Tell your parents yer doin a science experiment if you have too, they will eat that school shit up, you could probably do your whole grow by telling them it is for school.
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