Question about Invitro

03-16-08, 03:41

Teks I've been reading are saying that you should give your cakes light throughout the colonization process in order to speed up pinning.

I wonder about the logic of this, since giving cakes light slows down colonization.

slow colonization, quick pinning.

vs.

fast colonization, slow pinning.

It seems to me you're just trading the time of one phase for another.

Am I wrong?

Sunstar · 03-16-08, 03:46

Yeah it best to keep in the dark till completly colonized. IMO

scientia · 03-16-08, 05:05

I don't know...I seem to get about the same colo speeds either way. Just my experience though, and I've never done a side by side with an iso or anything.

I would THINK that the lack of cells touching each on the edge of colonization would be the major growth signal to the fungus. Moreso than light would slow it down. That and my incubator fills up really quick with using spawnbags.

It could also be species dependent too. I started a bag of cyanofriscosa that is growing pretty slowly, so I might try removing it from light and seeing if it picks up...

tr3eman9 · 03-16-08, 11:08

i was also wondering this...

hyphaenation · 03-16-08, 11:10

In a cupboard, in the dark.

but

You check your jars often, so there is a little light. Eventually they start to pin regardless.

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03-16-08, 03:41	#1 (permalink)
~CaptainZero Guest Posts: n/a	Question about Invitro Teks I've been reading are saying that you should give your cakes light throughout the colonization process in order to speed up pinning. I wonder about the logic of this, since giving cakes light slows down colonization. slow colonization, quick pinning. vs. fast colonization, slow pinning. It seems to me you're just trading the time of one phase for another. Am I wrong?

03-16-08, 03:46	#2 (permalink)
Sunstar Gazer of the innerself Join Date: Mar 2007 Posts: 2,095	Yeah it best to keep in the dark till completly colonized. IMO __________________ I'm just sitting here watching the wheels go round and round, how I love to watch them roll ~John Lennon~

03-16-08, 05:05	#3 (permalink)
scientia Mycotopiate Join Date: Jul 2007 Posts: 375	I don't know...I seem to get about the same colo speeds either way. Just my experience though, and I've never done a side by side with an iso or anything. I would THINK that the lack of cells touching each on the edge of colonization would be the major growth signal to the fungus. Moreso than light would slow it down. That and my incubator fills up really quick with using spawnbags. It could also be species dependent too. I started a bag of cyanofriscosa that is growing pretty slowly, so I might try removing it from light and seeing if it picks up... __________________ -knowledge is power

03-16-08, 11:08	#4 (permalink)
tr3eman9 Cthulhu-ite Join Date: Feb 2008 Posts: 252	i was also wondering this... __________________ "Don't you draw the queen of diamonds, boy, she'll beat you if she's able ... you know the queen of hearts is always your best bet."

03-16-08, 11:10	#5 (permalink)
hyphaenation Puck Teknician Join Date: Feb 2007 Posts: 6,905	In a cupboard, in the dark. but You check your jars often, so there is a little light. Eventually they start to pin regardless. __________________ This is the house that Hip built ! Best free university in the world

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