Spore print question

[rncd787]

Can I inoculate a jar directly from a spore print or is it unnecessary to put them in a blank syringe? I wanna say that I read somewhere that I could just use the back of a sterile butter knife to scrape some of the spores into the jar but now I cannot found that tidbit of info for the life of me.

[Hippie3]

it's possible if you know what you're doing.
got a flowhood or good glovebox ?
you'll need it.

[rncd787]

Well I don't really know what I'm doing as this will be my first grow but getting spore syringes is out of the question for me.

[IntotheLabyrinth]

Why not just get a syringe for "oiling some bearings on yer tractor?"
They sell the blunt tip ones at places like TSC, they aren't for doing drugs so you don't really have to worry about any suspicion.

[mestophilies]

lab is right, go to farm supply of any sort and get some syringes. If this is your first grow DON'T go straight spores into BRF. IME you WILL contaminate and then their are tears, cussing, etc.

[lucysd]

Originally Posted by rncd787

Can I inoculate a jar directly from a spore print or is it unnecessary to put them in a blank syringe? I wanna say that I read somewhere that I could just use the back of a sterile butter knife to scrape some of the spores into the jar but now I cannot found that tidbit of info for the life of me.

hehe sounds kinda like me hehe when i was just started and had no supplies,

on my b+ log those all started form lc that i made by scraping the print off the paper(thanks again hip, your turned me into a myco addict with my first print ever)
with a razor blade

but yes , if you are going to do that, the utmost care must be taken to assuer that your lc doesnt get contaminated

now remember, cause i couldave made way more lcs if i would have used way less spores per jar, sooo crape only the tiniest bit, if you see a little spore fall inthere thats more than enough casue they are microscopic almost so there are thosands of spores in there even if you can only see like a little one

i didnt even have a pc at the time so i used plastic ziplock containers

and i used a microwave

but like i said ubber sanitizing

clean the room you work in with bleach, thenlet it sit, then with lysol then let it sit, thebn with alchool, close vent in room, and if you have a glove box, do the same thing you did to the room inside of the glove box, use face mask and gloves

i get those free sample perfume bottles from macys and fill them with iso alchohol so that i can mist the inside of my glove box

but yea just scrape the tineist bit into each container

it should work providing you are uber clean

but lemme tell you get needles

cause they are way eisier in the long run

[rncd787]

From what I gather the technique used to suspend the spores in a syringe from a print is even harder than spore print inoculation. As far as the syringes I'm not worried about suspicion I have a couple safe addresses and the needles are way to big for heroin.

[lucysd]

Originally Posted by rncd787

From what I gather the technique used to suspend the spores in a syringe from a print is even harder than spore print inoculation. As far as the syringes I'm not worried about suspicion I have a couple safe addresses and the needles are way to big for heroin.

it just seems harder, honestly its way easier than it looks you just need to have a method that you are comfortable with

suspending spoers in a syringe isnt hard,

squirt steril water into baggie with print
rub print through bag with finger to get it into the water,

now suck spore water back out with syringe, there done

real easy, and way less chance for contamination than to just scrape things

[rncd787]

That sounds much less complicated then what I have read. Should I soak the bad in alcohol and run some boiling water threw the syringe a few times? How many times could a repeat this process to get more syringes?

[lucysd]

i would assume that the baggies come sterile out of the box, but the baggie that the print came in is the one you use,

so its already sterile,

but yes before i open the bag, i rub the outside of it with alchohol and i make sure it evaped befor i open it up

like i said, but maybe not in your thread, lets say someone gave you a print and you could barely see it, and you put enough water in there for four syringes,

and when you look into the light you cant see shit in there but the water

trust me theres spores in there and they will work heheheheehe. so what you can do is get a container of sterile water and scrape all the spores in there , mix it good with a sterilized spoon or some shit like a rod or whatever

and you just keep filling yer needles till you run out of solution

it doesnt have to be dark at all

ive knocked up jars of liquid culture and i couldnt see not one spore, it just looked like i squirted water, ha one week later i have nice lc

[rncd787]

Well I guess I'll do that then. I plan to do brf cakes via pf tek cause I don't wanna start to big. I plan to start a grow log with it so I can be critiqued by some of the vets around here but I really appreciates everyones input here other wise i would have wasted my time. And would flavor injectors work as syringes.

[zombiewoof]

Go to Publix and buy the 60cc luer lok syringe Cajun Cooker sells for injecting broth in your turkey you're frying. KISS

[lucysd]

well i dont really think soo

but if you order a print order some syringes with it,

i dont think you can pc the basting syringes

or something,

dont waste your time , get syringes

[rncd787]

I'm not ordering prints. Would I have to pressure cook them to sterilize them. Could I just run some boiling water threw them a couple times then flame the tip.

[lucysd]

well when you get the syringes they come steril outta the package,

and when you wanna reuse them just wrap them in foil and throw in the pc with whateverelse you happen to be sterilizing at the time

but i guess doing the boiling water thing works too

[FredTheSilly]

Originally Posted by rncd787

I'm not ordering prints. Would I have to pressure cook them to sterilize them. Could I just run some boiling water threw them a couple times then flame the tip.

While PCing is prolly a better method, I have had good success just boiling.

I take my old syringe, boil it in distilled water for a spell, then suck up some of the boiling water, shoot it out in the sink.
Repeat.
After the 3rd time doing this, I leave the water in the syringe.
Then let it cool to room temp...

Then I have a syringe filled with *sterile* water for spore syringe uses.

To make the actual syringe, I do what Miss Lucy does...

squirt steril water into baggie with print
rub print through bag with finger to get it into the water,

now suck spore water back out with syringe, there done

Good Luck

Fred

[rncd787]

Once I get my first harvest what is my best bet for re inoculation of more jars? Should I learn how to take my own spore prints to make more syringes or can I use myc growth off a verm cake to innoculate another jar. Seems like that may be highly vulnerable to catams

[FredTheSilly]

Originally Posted by rncd787

Once I get my first harvest what is my best bet for re inoculation of more jars? Should I learn how to take my own spore prints to make more syringes or can I use myc growth off a verm cake to innoculate another jar. Seems like that may be highly vulnerable to catams

Either will work

Personoly, I think making prints, and syringes is pretty easy stuff.
Besides, learning how to make sterile prints is the first step in building a strain library

See... http://forums.mycotopia.net/mycotopi...ie-prints.html (Free cubie prints)

I have never tried useing myc from a jar to shoot up more jars, so can't really speak to that.

Fred

[lucysd]

what i have done is far easier than getting myc from one jar and shooting it into another, but i use wild bird seed

all i do, is scoop some myc into a few other jars of wild birdseed, everytime i make my bulk sub, all you need is just a dab and you can keep a pretty nice cycle of jars, but i think you can only do that a certain amount of times, ive done it three times with sucess heheeh