Sterilization

rtorres · 08-13-08, 12:52

I sterilize the spawn bags completely sealed in a 25X All American Steroclave, however I have problems because with the increase of volume some of them blowup. Can I sterilize the grain in the bags open, or only semi-closed?

Myc · 08-13-08, 16:41

Are you sealing the bags with an impulse sealer pre-PC?

Try rolling the top down and securing it with a clothes-pin or binder clip.
This is how I do it and I've never had a bag blow up.

Beastmaster · 08-13-08, 16:56

Yeah don't seal them until after the sterilization. But like Myc said, make sure to fold and roll and clip the loose tops, so that after sterilization, you can just unroll the top a bit and seal it without sucking in any outside air.

sandman · 08-13-08, 18:09

i do mine unsealed, with the top neatly folded down and layed on itself. Use regular cheap scotch tape to tape the excess bag in place to itself. Then when the pc is cool, remove them directly into your flowhood stream one at a time. Fill the bag with sterile air from the flowhood AND innoculate it through the open top at the same time with G2G or LC or whatever. Grasp the top of the bag closed while trapping in as much sterile air as possible and twist it closed. Use a wire tie to seal the bag. This is the most reliable way I have found to do spawn bags.

goldenteacher1163 · 08-13-08, 18:27

If you do not have a flowhood you can buy spawn bags that can be sealed pre pc. Sporeworks has them

rtorres · 08-14-08, 09:48

yes, i seal it with a impulse sealer.

Myc · 08-14-08, 11:59

Problem solved then.
The filter patch is designed with gas exchange in mind
But it can't handle the rapid transfer of steam and hot gasses released by pc'ing.
The unsealed top acts as an over-pressure relief.

After cooling the bags can easily be handled in a glovebox.
I just used some clear packing tape to seal them after innoculation
So I could take them out of the glovebox and seal them properly.

Good luck!

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08-13-08, 12:52	#1 (permalink)
rtorres Mycophiliac Join Date: Jun 2008 Posts: 10	Sterilization I sterilize the spawn bags completely sealed in a 25X All American Steroclave, however I have problems because with the increase of volume some of them blowup. Can I sterilize the grain in the bags open, or only semi-closed?

08-13-08, 16:56	#3 (permalink)
Beastmaster Critter Keeper Join Date: Feb 2005 Posts: 2,113	Yeah don't seal them until after the sterilization. But like Myc said, make sure to fold and roll and clip the loose tops, so that after sterilization, you can just unroll the top a bit and seal it without sucking in any outside air. __________________ 'Any fool can make things bigger, more complex, and more violent. It takes genius and courage to move in the opposite direction.' AE

08-13-08, 18:09	#4 (permalink)
sandman Seasoned Pork Join Date: Apr 1972 Posts: 1,526	i do mine unsealed, with the top neatly folded down and layed on itself. Use regular cheap scotch tape to tape the excess bag in place to itself. Then when the pc is cool, remove them directly into your flowhood stream one at a time. Fill the bag with sterile air from the flowhood AND innoculate it through the open top at the same time with G2G or LC or whatever. Grasp the top of the bag closed while trapping in as much sterile air as possible and twist it closed. Use a wire tie to seal the bag. This is the most reliable way I have found to do spawn bags. __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

08-13-08, 18:27	#5 (permalink)
goldenteacher1163 myco guru Join Date: Dec 2006 Posts: 279	If you do not have a flowhood you can buy spawn bags that can be sealed pre pc. Sporeworks has them __________________ "A man shall never be enriched by envy." -Thomas Dreier

08-13-08, 16:41	#2 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 1,248	Are you sealing the bags with an impulse sealer pre-PC? Try rolling the top down and securing it with a clothes-pin or binder clip. This is how I do it and I've never had a bag blow up.

08-14-08, 09:48	#6 (permalink)
rtorres Mycophiliac Join Date: Jun 2008 Posts: 10	sealer yes, i seal it with a impulse sealer.

08-14-08, 11:59	#7 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 1,248	Problem solved then. The filter patch is designed with gas exchange in mind But it can't handle the rapid transfer of steam and hot gasses released by pc'ing. The unsealed top acts as an over-pressure relief. After cooling the bags can easily be handled in a glovebox. I just used some clear packing tape to seal them after innoculation So I could take them out of the glovebox and seal them properly. Good luck!

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