invitro bags: need some guidance

[livewire420]

ok so this is the thing. i see some stuff here on invitro but not much really. the only reason i think i really like the idea of this is:

1. you not not counting your chickens so to speak. meaning if one bag goes bad it's just one bag not a whole tub.
2. its great for people who doesn't want a million and one tubs sitting around the house and is easier to hide 30 bags instead of 10 or so tubs.

of course you can put tubs in the closet just like bags but i think i feel more comfortable doing bags. i do plan on doing a tub grow and a bag grow and see if for sure thats just it or if it's the fact i dont want to loose a whole tub compared to a bag. ok now i got that established.

i think it would be good for bag growers to post their recipe that they've tried or even how they prefer to do there bags. i know we got crazy1 and limeade that likes bags maybe they can add info to this thread that will help new bag growers such as my self with how to go about doing coir/verm bags, coir/verm/casting, hpoo/straw, hpoo/verm, hpoo/casting/straw/verm/coir/etc.

im sure you guys get the point. im gonna be regularly posting in here giving my experinces with the subjust cause as we all know i just failed pe. so im looking to improve what might be the problem.

all info welcome, and thank for the one who do give input on this matter.

[inkawgnyto]

I just nocc'ed up some BRF half pints with all intentions of having them go invitro.

I've seen some pretty neat invitro bags floating around, and have been watching the sand experiment closely.

Consider sand + supercake + BRF with your invitro mix, and just leaving the spawnbags alone.

[meanman420]

I knocked up some of Hippie's bags and am currently awiting fruits.One bag was nocc'ed up with 6 cc of b+ and was 100 % around the 7th to 8th day.It's day 10 and am seeing lots of knots.I duct tape my bags to coat hangers and then throw a shirt over them like a normal shirt in the closet.This helps disguise it and helps block light

[i patch]

tis a rh n fae thing. some strains a wee bit more sensitive i believe.. sometimes u get a bad phenotype ... just that thing u live n learn
i still tryin too

[limeade]

Originally Posted by i patch

tis a rh n fae thing. some strains a wee bit more sensitive i believe.. sometimes u get a bad phenotype ... just that thing u live n learn
i still tryin too

I can agree with this, there are some strains that would probably benefit better being in a tub.

i haven't had a monotub grow in some time because of the space, time, care, and attention they require, bags i can focus on more important things.

i would consider bags a huge success because they are little microclimates, they totally take care of theirselves, tubs u have to mist/fan etc..
i started noticing monotubs benefited more from care then neglecting it, they work with neglect, but they do better with care,

bags just work with neglect,
getting a proper filter patch bags is vital.

there are different types

3a is more for colonizing
3b is multipurpose
3c is fruiting only.

3b unicorn filterpatch bags are top notch.

coir substrates do work, but aren't up to par with using manure/straw based substrates or compost.

I haven't tried any of hippies mixes, maybe i will when i can find some time,
I am pretty lazy i order my substrates.

but that's the beauty of it, it works for all of us.

I am sure I can try to do my best to answer any questions,

oh, and use paperclips to close up your bags, after rolling it over a few times, tape gets tiresome, impulse sealing becomes bothersome, and unnecessary, lable and date your bags, mark the flushes.

k, off to update my penis envy log. It's invitro bag with compost.

[crazy1]

I'll get an invitro grow going today for ya.

I'll be using compost for this grow.
As I have a lot that I made. hehe

Now the strain will be PESH that I have in an lc right now.

Here I don't really hang em up in a closet or anything like that, they'll just sit on a shelf and grow from there.
It will be done in a filter patch bag noc'd up with the lc.

pics to be added tomorrow am.

Great thread Live

[livewire420]

so how can you tell the difference in what your working with?

there are different types

3a is more for colonizing
3b is multipurpose
3c is fruiting only.

3b unicorn filterpatch bags are top notch.

say from the the ones at sporeworks
Large Presealable Autoclavable Gusseted Bulk Spawn Bags w/ Self-Healing Injector Site #16250
Set of ten bags per offer (select larger sets below for bulk discount). Features microporous filter patch for contaminant free gaseous air exchange and self-healing injector site for syringe inoculation without taping! Ideal for spawning grain, sawdust, or straw. In many cases the bag can be used as the fruiting container. New model filter design allows bags to be presealed before sterilization. Measuring 18" high * 8" wide * 5" deep.


Large Presealable Autoclavable Gusseted Bulk Spawn Bags #16248
Set of 25 bags per offer (select larger sets below for bulk discount). Microporous filter patch allows contaminant free gaseous air exchange. Ideal for spawning grain, sawdust, or straw. In many cases the bag can be used as a fruiting container. New model filter design allows bags to be presealed before sterilization. Measures: 18" high * 8" wide * 5" deep.

because none of these say anything about what type of filters?
infact none of the sponsors here or at a few other place offer nothing but spawn bags.

[sandman]

The pre-sealable bags have type B filter.

[livewire420]

i just email sporeworks asking about this.

so then all of sporeworks bags are b filter cause they all have presealable written on them?

[sandman]

yup

[livewire420]

sandman is correct sporeworks just confirmed it.

[limeade]

Originally Posted by livewire420

sandman is correct sporeworks just confirmed it.

nice!

i've always assumed the ones with injector ports were for colonizing only.
But i make sure mine are 3b, if you "search forum" for invitro, you'll see numerous threads by me, "Invitro (strain here) on (substrate here).

[livewire420]

what do you gouys think about adding blood meal to a 50/50 coir/verm bag? Or do you think blood meal should stay in the compost pile?

and whats ya'll thoughts on spent coffee grounds?

[limeade]

I do add spent coffee grounds, it ups the (N),
I believe IF you choose bloodmeal, (i don't)
that you should use it very sparingly. it's very hot.

[limeade]

Live, if you need some more PE spore i can get it to you bro. I have a few coming up that look RATHER printable, very dark underneath. I'd like to see your penis in a bag, I updated my invitro penis on compost, i think you'll like the genetic stock.

[Phx]

Originally Posted by meanman420

I knocked up some of Hippie's bags and am currently awiting fruits.One bag was nocc'ed up with 6 cc of b+ and was 100 % around the 7th to 8th day.It's day 10 and am seeing lots of knots.I duct tape my bags to coat hangers and then throw a shirt over them like a normal shirt in the closet.This helps disguise it and helps block light

Holy crap thats one hell of a super cake formula. My FOAF nocc'ed his cakes on the 12 and now that I looked at them better I would say their only 8% done(just BRF ,verm and 1/3 cup of water per 1/2 pint jar). I am starting to think maybe he should look into that formula. I guess thats a prime example why its called a super cake...

[crazy1]

hahaha that's why it's called a supercake!

I'll post the pics of the 4 bags I have going here in an hour or two.
Gotta find the freakin camera lol

[limeade]

this thread isn't really about supercakes though, it's about invitro bags.

[livewire420]

yeah id love to get a print.

so when adding sent coffee how much do i add?

ya i never got to finish then bags out ended up moving cause of other things going on, but my 30 tc clone jars been ready to roll. plan is casting/coir/verm 2c of carb

i want to try out the coffee this time. if anyone knows the correct dosage i need to do.

lime- mail coming your way sorry it took so long

[crazy1]

well here are 2 of the 4 compost invitro bags of compost spawned with 60ml of pesh LC.



no colonization yet, but I'll keep this updated

[livewire420]

cant wait to see em thanks for sharing

[limeade]

those would colonize 5 times faster if spawned to and re sealed, instead of inoculatng with spore or LC, but yes, keep it updated!

Live I use a half cup of spent grounds per block of coir.

[crazy1]

Didn't have spawn, just an LC, so I figured wtf and went with it.
I do most of my cakes and bags out of LC.
Have good luck with em too.

hey do you add that coffee when mixing for the soak before pasteurizing

[limeade]

Originally Posted by crazy1

Didn't have spawn, just an LC, so I figured wtf and went with it.
I do most of my cakes and bags out of LC.
Have good luck with em too.

hey do you add that coffee when mixing for the soak before pasteurizing

I add the coffee DURING pasteurization.
You can use cakes as spawn bro, makes great spawn!
I wouldn't do supercakes for spawn as those seem to be used for straight fruiting and your bulk sub will have all that it needs!

[BuckarooBanzai]

Cakes are fantastically useful in this hobby, even if you never fruit them.

They make excellent spawn - a stand up vegetable grater will reduce a cake to a million points of inoculation in a very short time.

Cakes are also a very cheap and easy way to test an LC or questionable syringe.

[limeade]

cakes make great gifts for like minded friends, especially done ornately in a fishbowl on a bed of chosen marbles, i like choosing a bowl with a lid. i handed out a few for christmas last year, had it timed to where by new years 1st flush was coming up right on time for fresh doses on new years. The old hippies i gave one two thought it was just the best gift ever, especially since it kept giving, he used the prints from them to start LC's and then grains to do a lot of outdoor work on his farm.

[crazy1]

yup Buck cakes do make great spawn.
This is the mega cake I shredded up like you said to a bulk sub.



The cheese grater works like a charm

[BuckarooBanzai]

Gah - I know what you mean. I'm jacking his thread talking about cakes and have yet to mention my own fondness for spawn bags! Hell, I even wrote up a Tek about it.

Back on topic, damnit!

Total apologies Livewire!

BB-Nug Tek - popcorn/coir/castings in spawn bags:
http://forums.mycotopia.net/popcorn-...llon-jars.html (Buckaroo Bulk Nugget Tek (invitro, spawn bags and/or ½ gallon jars))

Invitro BB-Nug with her bag pulled off:



And man-o-man do I love those presealable spawn bags. So much less hassle. I don't use the injector port types, though. A blob of silicone with some duct tape over it makes a great inoculation port. Don't forget the duct tape, 'cause the silicone blob will sometimes pop right off the spawn bag without some support.

And don't put the duct tape on before the PC run. Duct tape melts...makes a sticky mess.

[limeade]

NICE BONZAI, while i know you supplied the link, and i will check it out, i believe i saw those while searching wormcastings, those have wormcastings right?

[BuckarooBanzai]

Dig it. Castings and coir. I like to add poly, others like verm, some just use the castings/coir. I think the poly makes the best water reservoir - and I hate screwing with the dunk...

[limeade]

Hah that's a most excellent tek!!

I just read through it!


this is what this thread needed, i simply follow hippie3's repost of hydr0420's method, i