yeast

marmy · 10-02-08, 23:30

What type of yeast does one use for liquid culture?

Myc · 10-02-08, 23:32

Nutritional yeast from the health food store.
Click on the highlighted hyperlink.

And a welcome to Mycotopia.
You'll certainly find what you need here.
Feel free to ask questions and browse our archives.

Dr_T · 10-03-08, 00:16

Before I knew about Nutritional Yeast, I used regular old bread yeast.
Worked fine. Either way, yeast is going to leave some sediment in your LC- don't let it weird you out. It belongs there.

marmy · 10-03-08, 09:06

Thanks for the information. Can one still use it when sterilizing in a pressure cooker? Will it still be effective?

Dr_T · 10-03-08, 09:14

Yes, you want it to be dead in your LC, it's there as food for the mycelium. So cooking it is required if it's a live yeast.

Raziel · 10-03-08, 13:27

You can use normal bread yeast ? I have some sachets of the stuff, how much water per sachet and how long should it be PC ?

Tried using honey before but it gets very cloudy, maybe yeast is better ...

Welcome to Mycotopia marmy !!!

Dr_T · 10-03-08, 14:40

I've never used yeast by itself, only with dextrose.
But with 2% dextrose, 10 ounces, I use a teaspoon of yeast. Or malt. Or half a teaspoon of each. Again, the yeast is there to provide some food for the myc, it's not doing anything yeasty.

But honeywater is a lot simpler, and shouldn't be cloudy if you do it right. Four grams honey to 100 grams water is the common recipe.

Raziel · 10-04-08, 08:52

Think I'll pass on yeast then. I decided to go with 1 teaspoon of honey and 400ml water recommended by Irishlion as he has had fantastic results with these measurments but I added the hony to the water and it turned a yellow golded colour is this normal ? It's PC 'ing now for 15 min, is this long enough ?

Dr_T · 10-04-08, 10:08

Don't know about teaspoons, I use grams for honey- but a light golden color is good. 15 minutes at pressure should be fine.

Raziel · 10-04-08, 15:10

Quote:

Originally Posted by Dr_T

Don't know about teaspoons, I use grams for honey- but a light golden color is good. 15 minutes at pressure should be fine.

Cool, mine looks a golden colour with the odd spec of material but im guessing its normal for honey or just a little caramalised sugar. Just added 2cm of spore solution to it

Does it have to be kept out of the light ? Dont want it to pin

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10-02-08, 23:30	#1 (permalink)
marmy Old School Join Date: Oct 2008 Posts: 175	yeast What type of yeast does one use for liquid culture?

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10-02-08, 23:32	#2 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 1,248	Nutritional yeast from the health food store. Click on the highlighted hyperlink. And a welcome to Mycotopia. You'll certainly find what you need here. Feel free to ask questions and browse our archives.

10-03-08, 00:16	#3 (permalink)
Dr_T Random Dude Join Date: Aug 2007 Posts: 1,731	Before I knew about Nutritional Yeast, I used regular old bread yeast. Worked fine. Either way, yeast is going to leave some sediment in your LC- don't let it weird you out. It belongs there.

10-03-08, 09:06	#4 (permalink)
marmy Old School Join Date: Oct 2008 Posts: 175	Thanks for the information. Can one still use it when sterilizing in a pressure cooker? Will it still be effective?

10-03-08, 09:14	#5 (permalink)
Dr_T Random Dude Join Date: Aug 2007 Posts: 1,731	Yes, you want it to be dead in your LC, it's there as food for the mycelium. So cooking it is required if it's a live yeast.

10-03-08, 13:27	#6 (permalink)
Raziel I don't walk, I float Join Date: May 2008 Posts: 610	You can use normal bread yeast ? I have some sachets of the stuff, how much water per sachet and how long should it be PC ? Tried using honey before but it gets very cloudy, maybe yeast is better ... Welcome to Mycotopia marmy !!!

10-03-08, 14:40	#7 (permalink)
Dr_T Random Dude Join Date: Aug 2007 Posts: 1,731	I've never used yeast by itself, only with dextrose. But with 2% dextrose, 10 ounces, I use a teaspoon of yeast. Or malt. Or half a teaspoon of each. Again, the yeast is there to provide some food for the myc, it's not doing anything yeasty. But honeywater is a lot simpler, and shouldn't be cloudy if you do it right. Four grams honey to 100 grams water is the common recipe.

10-04-08, 08:52	#8 (permalink)
Raziel I don't walk, I float Join Date: May 2008 Posts: 610	Think I'll pass on yeast then. I decided to go with 1 teaspoon of honey and 400ml water recommended by Irishlion as he has had fantastic results with these measurments but I added the hony to the water and it turned a yellow golded colour is this normal ? It's PC 'ing now for 15 min, is this long enough ?

10-04-08, 10:08	#9 (permalink)
Dr_T Random Dude Join Date: Aug 2007 Posts: 1,731	Don't know about teaspoons, I use grams for honey- but a light golden color is good. 15 minutes at pressure should be fine.