store bought button cloning

chupbracabra · 03-10-09, 22:23

Hey everyone!

Is it possible to clone a storebought mushroom? Or could I make a spore print from one? I purchased 4 fresh buttons very recently and was wondering what the best way to do this was, if possible.

Thanks

Soliver · 03-10-09, 22:30

Yes, and yes, sometimes depends on where the mushroom farm got / produced their spawn, but should work AOK.

A larger question is whether or not you're setup to grow buttons... it's really quite complex, too daunting for me,
personally, (I stick to oysters), but let us know if you work it out!

soliver

chupbracabra · 03-10-09, 22:35

okay, thanks! I think that I'll attempt to make a print out of two of them and try to make a LC out of the other/s.

chupbracabra · 03-11-09, 10:28

Well, last night I took a button, cut in half, and cut out a sliver of white mushroom from the stem, dunked it in hydrogen peroxide, and put it in a water+kayro LC.
I also cut another button in half and took a white part of the cap, dunked it in hydrogen peroxide also, and placed in a different LC.(This LC has water+kayro+pure vanilla extract)

Besides that, I took two more and cut away the veil, tore the stipe off and set them on foil to try and get some prints.

I'm hoping for some good results!

dfar · 03-11-09, 11:18

Quote:

Originally Posted by chupbracabra

Well, last night I took a button, cut in half, and cut out a sliver of white mushroom from the stem, dunked it in hydrogen peroxide, and put it in a water+kayro LC.
I also cut another button in half and took a white part of the cap, dunked it in hydrogen peroxide also, and placed in a different LC.(This LC has water+kayro+pure vanilla extract)

Besides that, I took two more and cut away the veil, tore the stipe off and set them on foil to try and get some prints.

I'm hoping for some good results!

I've recently tried to propagate button mushrooms in the manner you described but in my experience the LC didn't work very well. It is quite easy to get a spore print from the mushroom but i was unable to germinate the spores in a 4% LC solution, as well, direct mushroom tissue to 4% LC didn't show much growth either, turned brown/blackish after a week at incubation temps (80F maybe to hot?)

I wish you luck in your attempt and hopefully you'll have better results than i did.

on a side note i went back to the grocery store and bought a package of blue oyster mushrooms and a couple button mushrooms and am now trying to clone them with cardboard (I’ve seen a lot of success on the board with cardboard cloning recently) so if the LC doesn't work out for you then you might have better chances with that method, just a thought... so far my pan viet myc likes it, so why not button or oyster

chupbracabra · 03-12-09, 12:44

Thanks for the advice, I might try cardboard if I figure it out, and it's easy. I don't know anything about it really.

hyphaenation · 03-12-09, 13:11

Cloning it will be quite easy , but growing fruits will take some work. There are many threads that will steer you in the right direction. Its my understanding that compost is used as a substrate and there are other differences that set Buttons apart.

the_chosen_one · 03-12-09, 14:37

Quote:

Originally Posted by hyphaenation

Cloning it will be quite easy , but growing fruits will take some work. There are many threads that will steer you in the right direction. Its my understanding that compost is used as a substrate and there are other differences that set Buttons apart.

they require amazing amounts of CO2 to initiate pinning. air conditioned space is a must due to low temps. i've had some degree of success, but honestly they seem hardly worth the effort.. unless properly equiped.

AND

many large commercial outfits genetically tag their strains.. which means nothing to a personal use grower, but don't get caught selling them. tagged strains are often patten protected.

chupbracabra · 03-16-09, 11:25

okay, I tried to do an LC from some button tissue. I don't think it worked out because the tissue is kinda brown now and I don't see much(if any) myc growth. I'll probably keep the jars for a little while to see if anything happens, but if not I might just throw the water/kayro/tissue under a cow pattie and check it periodically. I'm not expecting any results though and don't really care too much because I've got some oyster culture on the way and they're supposed to be easier.

thanks everyone for your input, if anything becomes of this button LC I will post here.

zodd · 06-06-09, 12:56

sounds like your having lots of fun there. my first cloan attemt worked great. it was a shiitake that i bought from asda (walmart). but then not knowing at that point that they turned brown with maturity. i chucked it thinking it was contaminated.

any way i was just wondering if any of yal can help me with my oyster printing technique im not quight there yet a but im seeing my first little primordia and i want to make sure i get this down before i give it a try.

i was thinking that the uneven shape would cause me problems as usually i make table legs out of the stem and use them as a perch.

also is there a tell as to when the mushrooms are mature for printing.

sorry if these sound like dumb questions.

Lazlo · 06-06-09, 13:03

Use gill fragments from the mushroom. You'll need to use agar to grow out the culture, so you can see what's happening as far as possible contaminates living amongst the culture.

zodd · 06-06-09, 13:43

Quote:

Originally Posted by Lazlo

Use gill fragments from the mushroom.

huh??
hay lazlo what do you mean by gill fragments im good on the agar work, but i was hoping to be able to just rest a mushroom cap on some foil then frow that inside a bogie then disicate. i was just asking for advice on the precoces but youve lost me with this gill fragments stuff. could you please elaborate for this country bumkin.
is this gill fragment tek hard to do?

Lazlo · 06-06-09, 14:47

Take tissue from a gill and apply it to agar. This way you have live tissue (from the gill) and spores among that tissue that will germinate and grow with the live tissue. Spores of Agaricus can be tricky to get going alone. But with the live tissue growth of the gills, they'll fire right up and grow. There will be different growth patterns that emerge from the inoculation. Some will be fluffy, some will be rhizomorphic. Choose a rhizomorphic section and then transfer it to it's own plate to further grow out. If the culture's clean and healthy, put a wedge or 2 of rhizomorphic growth into sterilized grass seed. Grow accordingly and once you have a flush of mushrooms, then select a clone for your master.

Om shanti · 06-07-09, 12:42

Good luck, Chupbracabra. Keep us updated!

I would also like to try growing buttons. I have some readymade compost for buttons to use. I'm planning to try cloning directly in WBS after dipping in h2o2 inside a glovebox. Don't know if that would work. I'll start next month some time.

BTW, I realized some time ago it's not recommended to eat buttons raw. They contain some substances suspected of various bad health stuff... so they should be fried or cooked. A shame, because I used to love raw buttons with potato salad, and perhaps a good steak on top.

Lazlo · 06-07-09, 13:30

Use grass seed, not wild bird seed.

Om shanti · 06-07-09, 13:57

Quote:

Originally Posted by Lazlo

Use grass seed, not wild bird seed.

Thanks!

Then I'll have to think of something else to do with the big bag of WBS I got on sale recently... :-)

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03-10-09, 22:23	#1 (permalink)
chupbracabra Mycoexplorer Join Date: Jan 2009 Posts: 145	store bought button cloning Hey everyone! Is it possible to clone a storebought mushroom? Or could I make a spore print from one? I purchased 4 fresh buttons very recently and was wondering what the best way to do this was, if possible. Thanks

03-12-09, 13:11	#7 (permalink)
hyphaenation Puck Teknician Join Date: Feb 2007 Posts: 5,646	Cloning it will be quite easy , but growing fruits will take some work. There are many threads that will steer you in the right direction. Its my understanding that compost is used as a substrate and there are other differences that set Buttons apart. __________________ We're just flying by the seed of our plants.

06-06-09, 12:56	#10 (permalink)
zodd Maniacal Laughter Mwahaa! Join Date: Jan 2009 Posts: 586	sounds like your having lots of fun there. my first cloan attemt worked great. it was a shiitake that i bought from asda (walmart). but then not knowing at that point that they turned brown with maturity. i chucked it thinking it was contaminated. any way i was just wondering if any of yal can help me with my oyster printing technique im not quight there yet a but im seeing my first little primordia and i want to make sure i get this down before i give it a try. i was thinking that the uneven shape would cause me problems as usually i make table legs out of the stem and use them as a perch. also is there a tell as to when the mushrooms are mature for printing. sorry if these sound like dumb questions. __________________ who would win between a monkey and a dog?

06-06-09, 13:03	#11 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	Use gill fragments from the mushroom. You'll need to use agar to grow out the culture, so you can see what's happening as far as possible contaminates living amongst the culture. __________________ How can you have any pudding, if you don't eat your MEAT?

03-10-09, 22:30	#2 (permalink)
Soliver Deviant Join Date: Feb 2005 Posts: 1,784	Yes, and yes, sometimes depends on where the mushroom farm got / produced their spawn, but should work AOK. A larger question is whether or not you're setup to grow buttons... it's really quite complex, too daunting for me, personally, (I stick to oysters), but let us know if you work it out! soliver

03-10-09, 22:35	#3 (permalink)
chupbracabra Mycoexplorer Join Date: Jan 2009 Posts: 145	okay, thanks! I think that I'll attempt to make a print out of two of them and try to make a LC out of the other/s.

03-11-09, 10:28	#4 (permalink)
chupbracabra Mycoexplorer Join Date: Jan 2009 Posts: 145	Well, last night I took a button, cut in half, and cut out a sliver of white mushroom from the stem, dunked it in hydrogen peroxide, and put it in a water+kayro LC. I also cut another button in half and took a white part of the cap, dunked it in hydrogen peroxide also, and placed in a different LC.(This LC has water+kayro+pure vanilla extract) Besides that, I took two more and cut away the veil, tore the stipe off and set them on foil to try and get some prints. I'm hoping for some good results!

03-12-09, 12:44	#6 (permalink)
chupbracabra Mycoexplorer Join Date: Jan 2009 Posts: 145	Thanks for the advice, I might try cardboard if I figure it out, and it's easy. I don't know anything about it really.

03-16-09, 11:25	#9 (permalink)
chupbracabra Mycoexplorer Join Date: Jan 2009 Posts: 145	okay, I tried to do an LC from some button tissue. I don't think it worked out because the tissue is kinda brown now and I don't see much(if any) myc growth. I'll probably keep the jars for a little while to see if anything happens, but if not I might just throw the water/kayro/tissue under a cow pattie and check it periodically. I'm not expecting any results though and don't really care too much because I've got some oyster culture on the way and they're supposed to be easier. thanks everyone for your input, if anything becomes of this button LC I will post here.

06-06-09, 14:47	#13 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	Take tissue from a gill and apply it to agar. This way you have live tissue (from the gill) and spores among that tissue that will germinate and grow with the live tissue. Spores of Agaricus can be tricky to get going alone. But with the live tissue growth of the gills, they'll fire right up and grow. There will be different growth patterns that emerge from the inoculation. Some will be fluffy, some will be rhizomorphic. Choose a rhizomorphic section and then transfer it to it's own plate to further grow out. If the culture's clean and healthy, put a wedge or 2 of rhizomorphic growth into sterilized grass seed. Grow accordingly and once you have a flush of mushrooms, then select a clone for your master. __________________ How can you have any pudding, if you don't eat your MEAT?

06-07-09, 12:42	#14 (permalink)
Om shanti S.W.I.M. in H.POO Join Date: Jan 2008 Posts: 1,297	Good luck, Chupbracabra. Keep us updated! I would also like to try growing buttons. I have some readymade compost for buttons to use. I'm planning to try cloning directly in WBS after dipping in h2o2 inside a glovebox. Don't know if that would work. I'll start next month some time. BTW, I realized some time ago it's not recommended to eat buttons raw. They contain some substances suspected of various bad health stuff... so they should be fried or cooked. A shame, because I used to love raw buttons with potato salad, and perhaps a good steak on top. __________________ The most important thing is to find out what is the most important thing.-S. Suzuki

06-07-09, 13:30	#15 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	Use grass seed, not wild bird seed. __________________ How can you have any pudding, if you don't eat your MEAT?

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