Turn your spawn into liquid inoculant!

hi all

i wanted some ideas on this thought, what if instead of inoculating your bulk subs with spawn, you blended your spawn in a device and did a liquid inoculation of your subs,. the idea is if you break the spawn up to a hundred more inoculation points, it will colonize faster with less spawn. i was thinking like maybe a garden sprayer with a slurry of blendid spawn. if you can turn your jar of 100 inoculation points into a jar of 1000 inoculation points wouldent that help alot more?

[Irishlion]

here bro its already been done and yes it does work very very well


http://forums.mycotopia.net/spawning...hree-days.html (TV Guide to Colonizing a Quart Jar in Three Days)

[Mushrooms201]

Yes LC and breaking up/ blending spawn if not done correctly increases your chance of contamination. I tried Blending spawn in a sterilized blender and food processor and always had better results with less work using WBS. Sorry to be a downer. But work it out then teach me how to do it cheaply and easily. I have a 250 lbs oyster project cumming up and that would be gold.

After reading that post above I think I might have to give that a shot. Ive always wanted to soak or was my straw as a method of inoculation. if anyone has any links please pm me. ( hope that's ok)

Yea well with the use of hydrogen peroxide, contamination will not happen as long as the subs colonize in 2 weeks,. and that write up is for grain jars i am talking substrates here,. maybe that is a lil to detailed for the type growers here but i am just looking to add that edge of getting faster colonization with fewer spawn used., the idea is for people looking to go big, think outdoor bed sprayed with liquid inoculant, major colonization in rapid time,.. i know its possible just seeing if anyones done it and what would be a good sprayer to use??? one that can filter matter.

[Mermaidia]

maybe that is a lil to detailed for the type growers here

Are you talking about your idea or TV's thread? I'd like to believe that you aren't insulting us.

Using peroxide won't rid your grows of contams, it will slow down the fruiting process. Being sterile will help to prevent contams. I think you need to do some more homework.
Furthermore, the more you stress the mycelium out, by allowing it to colonize, then have to recolonize many times tires it out.

[goldenteacher1163]

If it is speed you are looking for go to the archives and look into super spawning worm castings into your bulk sub. I can't remember who posted it ( alot of people have done some great write ups ) but you can colonize bulk straw in just a couple of days because of how well you can break up colonized worm poo and the color helps identify when mixing with the staw allowing you to fully coat all the sub. Then when the mycelium recuperates it just turns the substrate white.

http://archives.mycotopia.net/discus...tml?1091561037

[sandman]

Originally Posted by mirakel420

Yea well with the use of hydrogen peroxide, contamination will not happen as long as the subs colonize in 2 weeks,. and that write up is for grain jars i am talking substrates here,. maybe that is a lil to detailed for the type growers here but i am just looking to add that edge of getting faster colonization with fewer spawn used., the idea is for people looking to go big, think outdoor bed sprayed with liquid inoculant, major colonization in rapid time,.. i know its possible just seeing if anyones done it and what would be a good sprayer to use??? one that can filter matter.

LOL! You may be delusional. If you could spray a field with liquid innoc then that is how legit commercial growers would do it.

Also your statement about h2o2 is a wrong. There are so many things wrong with it.....good lord.

[doodle]

hey guys, check this out. take a fully colonized jar of spawn perferably rye or bird seed. shake it up and brake it apart and then take a few syringes and fill em up with water and wrap them in foil and pc them for 10 minutes at 15 psi and inject em all into the grain jar and give it a good shake and then flame sterilize each needle as you tilt the jar and slowly aspirate the water out of it into each syringe one by one. when your done you can toss the jar or let it recolonize. but you end up with contam free LC syringes. used it many times and works perfect for grain LC's.

[lost_onabbey_rd]

yep.. Fhat wrote a tek using that exact same method some 4 or 5 years ago..

[lost_onabbey_rd]

as to the original poster

one reason liquid innoculants don't work so well is all the extra moisture really throws the water content of the bluk substrate off... it also makes a nute rich slurry that is open to bacteria contamination
if you do give it a whir though be sure to let us know how it goes

[doodle]

hey lost the first part isnt aimed at you, i know your speaking to the OP and you gave great advice.

using sterilized water doesnt create a slurry of nutes for contams to thrive on, and on the water content aspect. it should never be used on bulk sub but on spawn jars. I never knock up sub's. i spawn to em, and ive used up to 40cc of Grain LC in a quart jar with out throwing the water content off enough to hurt it. and yeah i seen fhats some years ago. i started using it becuase a guy Agar over at the shroomery showed me a way using silicon self healing injector jars to do it. and after trying it and havent getting a contam with it yet, i quit making sugar based honey/karo LC's to use on my grain to do my spawn runs

[lost_onabbey_rd]

exactly.. that method only works though b/c what you are innoculating with the syringe is in a sterilized container
point is even if you do make a slurry by tossing everything in a blender your still going to have the same number of cells that were in the original jar..

Originally Posted by lost_onabbey_rd

exactly.. that method only works though b/c what you are innoculating with the syringe is in a sterilized container
point is even if you do make a slurry by tossing everything in a blender your still going to have the same number of cells that were in the original jar..

yes same number of cells, but what if u cut 10 spawn kernals blendid and liquidfied into 500 live cultures floating in the solution. THE WHOLE ADVANTAGE of mycelium running is mycelium can be broken down into different cell chains and each one of these is a new site for mycelium growth, increase your divided cell chains and increase your points of spawn particles rapindly taking over a sub in a matter of days!

Originally Posted by sandman

LOL! You may be delusional. If you could spray a field with liquid innoc then that is how legit commercial growers would do it.

Also your statement about h2o2 is a wrong. There are so many things wrong with it.....good lord.

really and how so is that? thats how i do all my liquid culture incoulations (in a peroxide syringe). the subs would be pasteurized, fighting off contams, the liquid myceluim slurry would just be cleaned with peroxide ,.. this will very well work, peroxides just a barrier of extra protection but subs should colonize just fine

[doodle]

its going to contam before the whole sub gets colonized, you are maing thousands of more inoc points. no doubt about that. but if you simply saturate the top of the pastureized sub then it will never get the bottom colonized before contam gets ahold of it. i wish you good luck though

[lost_onabbey_rd]

by making a slurry with a nutrient rich substance like wbs not only are you breaking up the mycellium you are also breaking up the grain, exposing more uncolonized sugar and starch
also if your not aware of it, H2O2 will destroy cells it comes into contact with
the only reason you can dip a tissue sample in peroxide and it recovers is b/c it is more then 3 layers of cells, as the outside layers are killed by the peroxide, thus the increased recovery time seen with this method.
if you throw everything in a blender your gonna break it all up too small to make it threw a heavy dose of peroxide

but like i said.. feel free to give it a try and let use know how it goes..
i'd be willing to bet not as well as you think

[hyphaenation]

I noticed something interesting when fooling around with slurry. I attempted to try a 1/2 filled colonized grain jar. When I added the water and went to pulse the grains immediately float. Its even hard to get them to blend due to this floating action. Mycelium is freed from the grains and now floats below in the water.

This can be done without the blender by shaking the grain jars first and then adding the water. Shake again and flip the jar so your siliconed holes are on the bottom and let the grains float to the top.

Now the water is cloudy with mycelium. Suck up syringes full using a wide gauge tip. Its tricky doing it from underneath but the job is made easier if you set the jar on something to free your hands.

Sorry for the long explanation but I did want to mention that colonized grain floats , while mycelium sinks and the two can be separated with shaking & water.

[TVCasualty]

Originally Posted by mirakel420

yes same number of cells, but what if u cut 10 spawn kernals blendid and liquidfied into 500 live cultures floating in the solution. THE WHOLE ADVANTAGE of mycelium running is mycelium can be broken down into different cell chains and each one of these is a new site for mycelium growth, increase your divided cell chains and increase your points of spawn particles rapindly taking over a sub in a matter of days!

In any biological process we want to control there is going to be a point of diminishing returns for methods that go beyond a certain level of complexity and expansion rate.

It's hard to say where that point is until the limits are tested. Lots of smart people with big budgets have been growing mushrooms for a long time with a focus on maximizing yield while minimizing the time, energy, and resources needed. Before trying to theoretically re-invent the wheel it might be more productive to study the current methods used by commercial (and skilled amateur) growers. Trying them out in an actual grow would be even better.

Once your colonization times and yields approach those of current commercial growers you'll have a much stronger foundation of knowledge and understanding from which to design theoretical improvements.