Cloning and cardboard idea, need some advice

[ishkaboo]

I am carrying out hypaenation's cardboard cloning machine tek (great write up btw) since a giant fruit popped up and I thought I would try something new, and I had an idea but didn't know if it would work. As can be seen here http://forums.mycotopia.net/photo-ga...g-machine.html he says to use WBS and use the grain as spawn, but I have decided I want to use one of the jars (I used pints) to fruit straight from a cake, and was wondering how bulk materials would do as a replacement for the WBS? What if I pasturized some straw/coir/verm and put that in the jar instead of WBS and let the cloned tissue colonize that, then birth it as a cake once fully colonized?

The way I figure it, the pasturization process slows down any baddies in the sub at last a week or so, and given that the cloned tissue is actual mycelium as opposed to spores which have to germinate, I thought it would colonize fast enough to not get contams plus yield more than if I did the same with BRF/verm... Sound like it would work to you?

[Copernicus45]

you would have to soak the straw in some horse manure water, or use some type of manure in that mix because the clone cutting wouldnt colonize to good on just straw/verm/coir. You could take the cutting and put it in a jar of popcorn, then enject some sterile water and make a mycelium slurrie that you could use to knock up some jars.

[hyphaenation]

The reason for using wbs or other grains is so that you can quickly do a G2G once colonized. Now you will have a dozen(s) of jars full of your clone. Next you can spawn to bulk with that.

For an instant cake I made an adaption where I do the following:

-Cut cardboard disks , soak them
-Fill jars with BRF mixture , leaving a small gap on top
-Place cardboard disks on BRF (place 3 or four until its right to the top)
-Make special lid. Cut plastic lid off pop/water bottle ( 1 + inches) , cut hole in jar lid so that bottle lid fits in and protrudes down a tiny bit into the jar , silicone that plastic piece under and above. Let dry.

Now you have the BRF , the cardboard disks to the top , the special lid with the snorkel. In the snorkel place polyfil stuffing. Cover the whole lid with tinfoil and PC.

When that cools you can take the jars into your glovebox. Remove the tinfoil and drop a clone chunk down the snorkle so it lays on the cardboard. Replace the polyfil into the snorkel.

Then its a matter of waiting several days for some mycelium to form and transfer on to the cardboard. As soon as you see some good formation of myc you can take a toothpick and pluck out the clone chunk from the snorkel. This is to help avoid contams as the chunk starts to "rot".

Always limit the time that you have the snorkel free of polyfil and try and work in a glovebox wherever possible.

Sorry I have no pics of this adaption. I'll try and get some the next time I use it.

Regarding your question about straw and such , the only problem I see is birthing the cake after its colonized. It would be a bitch to get out of the jar (unless you used something like plastic yogurt containers).

Best of luck on your projects.

[ishkaboo]

Thx, its sounding like I'll just use grain to spawn to bulk.