Panaeolus tropicalis beginner

[nyi]

I have some experiences with P. cubensis (not so much, I've done 2 successful strains, the third is already forming knoths and many partialy (un)successful experiments with different species). Now I would like to see Panaeolus in my terarium (Why? It doesn't matter, I just believe, they're the way.). Never used other substrates then grains (I used wood for azures though), so I have a few questions.

I had 2 unsuccessful tries with spawning dung. I _tried_ to pasteurize horse dung, which contained much of the black goo. I tried to pasteurize it with minimum of boiling water, just enough to soak it in. second try was with sterilized dung/vermiculite/coir mix and pns are _very_ slow on it and it will contaminate fast, because the jar cracked. None worked, I wanted to concentrate to do the third 'non-experimental' inoculation.

I took some (about 5 litres) of dung and soaked it in plenty of water with a little bleach and cracked all clumps. The poo tea was drained out overnight and the poo is now drying in the sun (third day).

1. Should I pasteurize even if I used bleach? (Hopefully the bleach has not hurt the manure, some slow white mold was growing on it of two days, while it was in storage)

2. Can buffer increase the quality of manure/verm substrate for the pans? Aren't they acid loving, or they don't care? I use buffer only for casing, where I want to avoid 'The Bitch' Trich normally

3. I got my spores from fsre.nl. Can it be true, that Pans cambos/trops are instead Pans cyanescens? I can't check microscopic features yet

[Foster]

Bleach pasteurization has been successfully used many times. However if you had a mold growing, you might consider a hot water pasteurization also.

I like to use a pillowcase of poo in a cooler, add a pan of hot water. Making sure its around 165f for at least an hr. Let it sit overnight, squeeze out excess. simple and effective .

As far as a buffer, never used one. Might be beneficial if you are using a peat based casing.

Good luck with that pan cambo, they are something to behold.

[nyi]

Great, thank you. I'll do the pasterization, that's not so much.

PS: They're Pans tropicalis Sandose. I don't know what is true fo rnow, so I believe that Pans. tropicalis are really tropicalis and not cyanescens, nor cambodiensis.

[nyi]

Some pics, just to show, how they're going. The first is my new spawn, kolonizing about 5 days. I don't know if I would use so much, but just for every case.

The second is pasteurized horse manure/verm. I put it on a thin layer of wet perlite (just to cover the bottom) and spawned with millet (which had contamination, I leached the millet with 0.1% peroxide and left it to colonize all of the spawn) as you can see, it worked. This pic is about 5 days after spawning. It was cased yesterday with coir/verm, just enough to cover the surface

Third is unleached, sterilized horse manure/verm/lime, which I left to colonize for about 3 weeks. I put it in casing without perlite on bottom, cased as above. I gave it no time to sit after I poured it into the tray and cased it right away.

They do very well. Hopefully they will do well in the same incubator as the cubies. I'll increase the air exchange, after the casing colonizes, mist the cubies a little bit more and don't mist the Pans at all and let the casing dry out.

[nyi]

Seams strange. The cased Pans in the small tray didn't colonized the casing. Maybe too much lime in casing. The other Pans, which grown on sterilized manure/verm/lime mix were cased the next day after my last post. Now it's day 5 but they don't show any signs of primordia formation. The substrate only shrinks a little bit.

Maybe they need a casing with biological activity. I'll try to recase the small tray with uncolonized coir/verm with peat/verm mix, this time without lime. The casing will be pasteurized.

I added an aquarium bubbler submerged into a flask with water and perlite flowing on top of the water layer. It's a cheap substitute for cool mister, which I can't get around here. Temp are in between 23C-25C (73,5F - 77F), humidity 85%-95% and there is a lot of fresh air. Hopefully I did not done it wrong.

No pics to post today. Only the grain colonized really strong, defeated some trich starting in a few points on the grain. I'll add some pasteurized manure with lime to the top of the jar, case and try to fruit right from the jar.

All advices will be welcome.

[spacecake]

How much lime did you use,it's should not be any problem.
How does the mycelium look if you look under the casing layer ?

[spacecake]

Originally Posted by nyi

mist the cubies a little bit more and don't mist the Pans at all and let the casing dry out.

? I just read this..you mist the cubies but not the Pans ?
And you let the casing dry out ?

Pans like more humidity,so would this be the opposite of what you are doing,or am I mistaken...?

Btw please post some pics of you can...

[nyi]

When I made this casing I added a few tea spoons of lime into 720ml jar. I fill the jar full of water when I add ingredients, because I have only a pen type pH meter at hand. The pH raised to 9,5! I had to wash the casing and add a drop of vinegar to lower it to about 8, or 8,5, I don't remember.

However. I read some threads about Pans grown by novices. They misted, because they believed they need humidity, but a few experts told them to stop misting, just maintain humidity and FAE. I do that. I realized I had very little water in perlite in bottom of the terrarium. I added as much water as I could and now I have 95%+ Rh... sometimes 99% Rh.

But I do mist the casing only if it looks dry. Just a little, enough for the coir to look darker again. I think that in the terrarium aren't good contidtions for Pans, nor cubies. Maybe I should build another terrarium (that wouldn't be a problem).

Another problem is, that the terrarium is in kitchen, where are big humidity and tempereture changes. Once, when I was sterilizing something and pasterizing at the same time, the temp jumped to 29C = 84,5F. I have to stay a little bit stealth and I don't have better conditions anywhere else at home.

[nyi]

And yes... I forgot. The dung under the casing is fully colonized, some fluffy mycelium grows out of it. It's shiny white and looks healthy and looking for some more food. I'll post some pics, hopefully today... I still promise some pictures and then I don't find time for that.

[spacecake]

Well those experts that say don't mist,do they use a humidifier to maintain the humidity ?
Btw a couple of teaspoons of lime is indeed a little to much,one teaspoon would do the job just fine...
But don't use vinegar ,you could have just washed it out with water.

[Lazlo]

I'd strip that casing off and would start anew. A quart jar's worth of casing only needs a tiny pinch of lime. Around 5ml's worth. Not even that much really.

Put casing down at a depth of 1/2". Once the casing has even mycelial growth across the surface (can be achieved by patching over early growth with a thin layer of casing soil), mist the casing to saturation and then put back into incubation for 24-36 hours. Once the mycelium recovers and is nicely visible across the casing's surface, you can put into fruiting conditions immediately. This way the casing is at peak moisture holding capacity when you switch into fruiting gear. Keep the rh at 95% and temps in the 75-78 range.

[nyi]

I think we don't speak of the same thing... with lime I don't mean calcium hydroxide, but calcium carbonate... ground limestone. CaOH does change the pH rapidly, but limestone does slowly, that's why I used so much... and overdid it.

Ok, there are the images. I have the pasteurized grain spawned manure in the smaler tray with perlite on bottom. The sterilized agar spawned manure in in the bigger tray without perlite on bottom.

[spacecake]

I know lime = calcium carbonate...
Calcium carbonate isn't slow,it's works after 15-20 minutes or so...
Did some test on that one...
I think it works shorter then Hydroxide in the long run and is less powerfull,but that doesn't mean you need so much of it...

Lazlo doesn't use that much,either,so trust me.. a teaspoon is plenty...

Next time add some lime,let it do his thing,and measure the Ph only after 20 minutes or so.

[spacecake]

Pictures are alittle blurry...
If you look at picture 3..at the bottom of the casing,is that mycelium,or has it dried out ?

[nyi]

I was not sure that lime = CaCO3, but thanks for the advice, it's very appreciated and I trust you, because I'm seeing your posts here and there for some time on mycotopia. 1 teaspoon on 1 liter of casing, wait, drink coffee, smoke, eat some pills, whatever what takes at least 20 minutes and then measure pH.

On the image #3 is no mycelium. It does dry out faster then the other casing I made, maybe the mycelium is hungry for water after I didn't misted it. The but this uneven color of the casing is normal... I left the verm in the oven a little bit more then normal and part of it darkened (I don't remember if it was the top, or bottom part). As you can see, it was not mixed with coir enough to distribute different particles evenly in the casing mix. I would say that's not a big problem.

I think now, after I was watching some Pans on grain in jar, that they didn't colonized the casing because of too little water. I read recently and it's obvious from pics of fruiting pans, that the casing should develop overlay to create fruits (or did someone suggested it in this thread?). Fundamental error, I thought that the same casing rules apply as with cubies. I hate overlay, no matter how hard I try, I get it. I tried too hard with pans now... lol

What an idiot I'm... I'm growing mushrooms 4 months now and think about myself as if I deserve the honour of fruiting pans in my home Ok... I'll try first to not to disturb the fragile mycelium, which started slowly colonizing the casing. I'll mist it to saturation, not wet, just moist, cover with thin foil and back to the incubator they go. 30C = 86F. Next station: overlay. The the temperature will go down to about 25C = 77F.

And... yeah... I know, I had better to pic up some TEK... Today I'm starting with Waylit's Pans cyan TEK.

[nyi]

Originally Posted by spacecake

Pictures are alittle blurry...
If you look at picture 3..at the bottom of the casing,is that mycelium,or has it dried out ?

And I forgot to mention... someone put my camera onto a wrong charger, so it's not charging, nor function right now I have to take these pics with my phone... It's a relatively good camera, but I can't be in hurry with her.

[spacecake]

Originally Posted by nyi

I read recently and it's obvious from pics of fruiting pans, that the casing should develop overlay to create fruits (or did someone suggested it in this thread?

Yeah,is it or isn't it good for the development of the pinset ?
I still haven't figured it out myself.
Some say it does,some say it doesn't.

I have seen allot of proof that both statements are correct.
BleuHelix for example says, that the Goliat strain likes overlay and some other strains don't...,but why ?
Maybe he just had a good genetic strain...fresh print or whatever.
Aggressive grows..,most of the time results in a good pinset but also overlay.
Does that mean overlay is a good thing ?

I hope to find out some answers I still have sometime...
Cubies I can do blindfolded,but Pans still has some secrets.

Well it sure makes this hobby interesting for a long time to go.

[nyi]

You're right spacecake! It's interesting and made my way to other related topic: edibles, botany, joghurts, biology. It's something for the big children IMHO.

Cubensis si great if you're looking for easy fruits. Personally I wanted to continue with some woodlover, then sclerotia formation, then fruiting sclerotia formating species... but all of these need time and I was left with too much free time. And now I ended with 5 projects, which already overwhelm me (which is good, because they do better without me) and 3 more are on their way.

... interesting is a good word!

Ok, what is the chance that I selected 2 non-fruiting strains from agar plate?

[the_chosen_one]

Originally Posted by nyi

Ok, what is the chance that I selected 2 non-fruiting strains from agar plate?

slim to none. even grabbing one non-fruiting is not very common.

there's a pretty good chance you grabbed more than one strain unless the myc was very clearly sectored.. which is rare with pans. it may require re-isolation to get it down to one good strain.. but there is nothing wrong with a two to four strain isolate.. it may offer up some real good choices for re-isolation.

[nyi]

Selecting strain from agar is not my prefered method. Cloning seems to provide faster and better results. I see that the pans strains are crosing each other on agar, almost imposible to select only one, maybe after a dozen selections. I should better say what is the chance of selecting two non-fruiting isolates. Doesn't matter.

I have an imitation of cool mister build in my FC. It's a bubbler running air through tall flask filled with water with perlite on top. I bought another stone today, so I have now double the FAE as before. Outdoor temps dropped, so I have a little bit better conditions for cubies, but pans would probably profit from higher temps. Right now I have Rh 97% and 24,8C = 76,6F. Hope this will change things.

I should better build a second FC for pans and a thermoelectric cooler for the cubies. Or maybe I should better follow some TEK and don't invite hot water over again

[nyi]

Ok... nothing happened in the few days (1-2?) of better humidification and ideal temperatures... they go back into the incubator. Is there a posibility, that the casing layer got contaminated by invisible contamination (bakteria, yeast)? I doesn't smell not right.

[spacecake]

That can happen...
Are you puting it back in the incubator with a new casing layer ?

[nyi]

I'm putting it back in terrarium with the same casing layer. I had not very much time in the weekend and I would really like to see, if the problem is really in the casing. If not much growth will be seen tomorrow afternoon, I'll recase them. If the casing is ok, then I would not like to disturb the fragile mycelium.

And yes, of course... I bought some 6400K 5W fluorescent light bulb for my terrarium. It doesn't get as much ligth as in winter (why thet deosn't matter). A day after I put it in the terrarium the cubie pins, which rested and grown mycelium in their caps, started grow.

I'm almost sure, I had too little light in terrarium. Probably, at least the Thai strain, needs a little bit more light to be sure, be sure that right now is the best time to fruit. Of course, because the pins appeared, the mycelium was just waiting and it would fruit after a few more days. What do you think?

[spacecake]

Well i'm a little confused...
I do not speak English very well,and I can't understand all of what your saying.

You talk aboud cubies ,lighting,winter/summer ,agar,cloning,terrarium/incubator...EVERYTHING !

lol ,don't get me wrong,but I'm totally lost here...
Maybe someone else should answer your question,because I can't.

[nyi]

Hehe... sorry... I don't think I really need all those answers... if other found ways to do the job on their selves, I should too. A big "thank you" spacecake, you were very help full...

After about 24hours of not looking on the cased pans, the bigger cake did some work.

I'm proud now to present you... The Pin:



The cake is back in fruiting chamber and I won't sleep in waiting what will I find tomorrow. Hopefully it won't be small or aborted and hopefully more pins will appear. Next there is cloning on the schedule and it will go right into the Waylits pans cyan TEK.

[Dr.Hallucination.]

Congratulations on your first Pan pin nyi

[nyi]

Thank you all! Your suggestions helped me a lot.

Today morning another one popped out. Yes, they're fast, I looked at 1:30am and in 6 hours there was another. But the battle is not at the end, they have to start maturing. The first didn't grow so far, but hopefully it will later today or tomorrow. I really don't know how they act normally, so I can not say.

One of my cubie casing got contaminated by some mold... propably. I don't really know I had never seen this mold, it's very white, like mycelium, but has different structure. I noticed the structure difference using magnifier. It looked like normal fluffy mycelium, but after inspecting different parts of growing mycelium, I saw that the mycelium growing out of same edges and pins is not growing in one direction, but changing the direction after short lengths of growth.

This isn't normal cubie behaviour AFAIK, not even Pans behaiour... it's cobweb behaviour, but with very white mycelium. I cut out the contaminated parts, sprinkled with a little salt and sodium bicarbonate and recased the cake. The cut out parts are saved for outdoor attemp in an PS container covered with tinfoil. The pieces will show very fast what the grow really is. I expect darkening of the contamination mycelium this afternoon.

I see I really have to clean and rebuild the terrarium. Nobody here saw the pictures of my FC, but believe me, even if it works it could be assembled better. A loose cardboard tray lined with plastic and filled with perlite is minimum, what could be done. I would like to ask what can I do with the heat of my new light bulb. How do you cope with excess heat in FC in general?

[nyi]

Ok... 3 pins! One is maturing. All those pins are close to each other. Does thin mean a better fruiting strain? Or only better condition at this side of the cake/terrarium? Wish me luck, to clone a good fruiter

[Om shanti]

Congratulations, and good luck with the clone!!

[nyi]

Ok, my progress so far is seen in attachment. Another small fruit is coming from below opposite side of the tray from below of the tray.

I think, I still didn't mastered the casing mysteries and all my cased trays are fruiting mostly from the sides. Not only Pans, but cubes too. Should I mist them more often? Maybe I really only add too much limestone? Or should they be thicker (for cubies I use 1cm = 1/2in).

The smaller tray don't show any signs of colonization of casing layer, but I didn't had time to recase it. Hopefully today.

I don't remember if I mentioned the copelandia on grain jar. I added pasteurized manure on top of the jar and packed it gently, leaving some space for casing. In about 3 days it was ready and I cased it with thin layer of the same casing mix as the above trays.

This time the mycelium looks... say really fast and healthy... maybe they need some time to colonize enough substrate to lift off, or in my other tries the substrate was contaminated with bacteria. Copelandia shows great potential in defeating contamination, but it needs its time for this job.

[chimp]

Those don't look like tropicalis to me.

[nyi]

Haha... Today I looked on the sporeworks website. They found out, that Sandose, which they sell are actually Pan. cambodigensis and renamed the Pan. trop. Sandose strain to Pan. cam. Sandose.

I have my print from fsre.nl, where prankster first donated these prints. These prints, which originated from sporeworks, are probably in circulation within Europe and through fsre.nl.

My pans look the same as in this thread Tropicalis Sandose Domesticate
(started by prankster himself).

More over, I really don't understand, how you can distinguish indoor grown pan. trop. and pan. cam. AFAIK, they look the same macroscopicaly when grown indoors and similar microscopicaly.

Never mind... want more pics?

[chimp]

I never said they weren't tropicalis. I said they don't look like them to me.

They very well may be.

The stipe looks awful thick in relation to the caps and the cap shapes aren't what I am used to seeing with pans, tropicalis or any pan for that matter.

No biggie, maybe it's the camera angle..just making an observation.

[Workman]

Originally Posted by nyi

Haha... Today I looked on the sporeworks website. They found out, that Sandose, which they sell are actually Pan. cambodigensis and renamed the Pan. trop. Sandose strain to Pan. cam. Sandose.

Yeah, sorry about the reclassification. Splitting out the Copelandia into species is really confusing, but I think I have a good handle on it now after examining dozens of strains/species.

The easiest way to distinguish cambodigensis from cyanescens is by the coloration of the pins. Pin coloration is more consistant than the adult coloration which can fade and change as the mushrooms mature. Cambodigensis pins are dark, sometimes extremely dark chocolate brown or sometimes olive brown. Cyanescens pins are pale yellowish, white or buff.

As far as I can tell, authentic tropicalis should be pale like cyanescens but with smaller spores and some other minor microscopic differences. So far I haven't seen a convincing specimen. I have seen bisporus which at least in the example I examined had dark colored pins. Oddly, the bisporus seems to prefer cooler temperatures around room temperature for best results.

[nyi]

They're really weird, it's not the camera I saw something similar but more pronounced in another grow report, but with pan. cyans. Slow colonization, inability to overlay casing, delayed fruiting, frequently changing fruiting conditions and small mushrooms were present. I would say, there is some bacterial contamination present. The petri dishes and substrates were held in very non-sterile conditions... Maybe I have much more bacteria contamination, then I know.

Workman, I have great respect for your work. It's not a shame to misidentify a mushroom specie especially when identifying tropical panaeolus species. It's great to have someone who invests so mush time into these gifts of nature.

In this occasion I would appreciate any tips from you how to improve my pans fruiting condition... I'm not experienced grower, but I'm learning and trying very hard and fast as I can...

[Workman]

I used to have all sorts of issues with pastuerized substrates and Panaeolus species. I know it can be done and done well, but I wanted something more bullet proof and smaller scale. I now sterilize quarts of horse manure and steriley add agar wedges of clean Panaeolus mycelium. The jars are usually colonized in a week. A liquid culture is even faster but I rarely use those. Once you have fully colonized substrate, its relatively resistant to contamination. I just put the colonized manure into a tray and apply a very thin sterile casing. This usually pins in 3-4 days with mature fruits in a week or so.

There are definate differences between strains so try a few before giving up. The easiest and most forgiving strain I've worked with is the Sandose, but it really depends on your growing environment and finding a strain that will perform for you. I have great luck with the Goliath strain, but I've heard plenty of reports of it not working well for everyone.

Good luck!

[nyi]

Thanks workman, that's very useful info.

First two fruits matured and dropped spores. Just in the time I expected them and made myself ready for cloning, printing and bio essaying (maybe I was too excited). This specie is distinct, no body load, visual and mind blowing and of course very potent.

In attachment is picture of the tray right before an early harvest. 24 hours later there ware 6 new pins and 3 more popped out through night. With my phone camera I can not do sharp pictures of the tray, but maybe this eveing. Very amazing genus, this panaeolus is...


First image is the tray before harvest, second is a closeup to the fat fruit I cloned and third are some new pins.

[spacecake]

Nice biggun's !

Hopefully the cloning will work for you !

[nyi]

I was busy in the weekend... my copeladias too...

1. Two days after pinning (morning)
2. Gills of mature fruits (in a hurry, problems with focus, sorry)
3. Three days after pins (morning, harvest, printing, in a hurry, bad pics, bad lighting, sorry)
4. The first pins from 2nd flush (there were actually 6 pins)

Hopefully there will be a third flush. From the first flush I cloned the stem and cap from inner flesh. The stem part contaminated with yeast and mold started growing 2 days later.

The cap stand still on its agar, first it darkened, but this morning it was light-grey-brown again... hopefully this is a sign of regeneration, it would be great to have a clone of a fruit, which had 6 grams fresh!

I have a question. As you can see, majority of the fruits grow from the edges, even from below the substrate... Did I do bad casing again?

[Foster]

Wow, those are the fattest stems and some of the largest caps I have ever seen on any pan. Amazing a '6 g pan, thats unheard of, at least to me. Very cool.

Im not doubting you but, I have to agree with chip, It doesnt look much like a pan cyan/ cambodgin. Is it possible the print was mislabeled? Did they turn almost black at points where they bruised? What color do they print?

Awesome specimens regardless.

Just incredible size man . It would sure be great if you got a few prints taken and would be willing to swap or send a few out to a few folks with pan experience. ahemm. lol (Foster raises his hand )

Not the best pinset and it does seem they like the edges, but still fantastic job. Maybe try a bit less or no lime next time? Like I said I have never used it, but dont usually use peat.

Congrats man! Foster

[nyi]

I'm surprised by they size too. The 6g specimen came from the first flush. I got the print from free spore ring and they were nice prints obviously done by someone experienced (Thick tinfoil, clean, flat, geometrically precise), the one who made the print so much carefully wouldn't mislabel it, but wh knows. This is a multistrain isolate. I took a while, to get to this point.

They bruised nearly black, print is dark-brown-purple, I'll look again, to be sure, but I'm 95% sure. The bigeast specimen from this flush weighted about

All in all... which other genus would at dose of 9g fresh, turn your room into a terrarium of "colourful snakes" crawling on the walls, create an crazy grin on your face, make you extremely gigly and hard to not burst out laughing (when your GF sleeps next to you), thinking extremly deeply about your problems and the world, little disoriented and without the cubensis body-load? The trip lasted 6 hours.

The bad side of this experience was what I call "overwhelming of senses" (which is something else a little bit), or more descriptive "telepathic explosion". It happens when your input stop changing (you don't move, and look at the same point in quiet place). A loud sound starts gaining volume fast in your head, you lose visual input and feel strong body feeling. This can be interupted just by realising what's going on, or moving, looking somewhere else, etc... just changing your inputs. It's not very pleasurable, I don't feel ready for what comes after it (schizophrenia maybe?).

I experienced it on smoked spice and harmala tea before, but not on shrooms, not even on 5+g doses of cubensis, or 6(+2g cubes) trips.

In the casing I used coir/verm (not peat) with excess lime, as you point out. I measured the pH of about 8.5, still a little bit high. I would use less, but I would not like to risk now, when I had an outburst of Trichoderma in my flat.

I printed all mature specimens and will share them, but if you're from outside of Europe, I'd better find someone from inside EU, who would reship them for others. I hope the clone will survive and I'll make more prints of this monster strain.

The biggest fruit from second flush weighed only about 4 gram, I didn't remembered it right, but that's pretty enough, I'd say. Maybe workman could say if the Sandose strain produces such big fruit normally.

[nyi]

Oh... I forgot to mention, that I have a 5W 6400K light in my terrarium, which is only 56x36x24cm (22x14x9.5in) and temps 26-29C (79-84F), high FAE and Rh 95-99%. This could contribute to the dismension of the fruits and maybe to the pinset.

[Foster]

Awesome trip experience man, yep sure sounds like pan potency. But all the pan cyan type prints Ive seen were jet black. Interesting to say the least. Might explain your fruiting difficulties if it is something different. Maybe workman will take another look and give his opinion on the matured fruits. I sure hope its pan trop though, sweeeet.

Hmmm a member from EU. Spacecake to the rescue maybe? lol

Even If I'm not able to get one there are a few members here who would definitely rock that print. And then maybe it would get around eventually.

Good luck with the clone, I hope it does well for you. Again nicely done and congrats. Those are some sweet looking specimens, you should be proud of your first pan attempt. It sure puts a smile on my face just to see it.

[Om shanti]

Looks great, Nyi. ;-)

[spacecake]

The print didn't came from me...
I've only send some cubie strains to FSRE and a couple of Sandoze,but those were printed on thin aluminium.

They look the same as I have grown them ones for the contest.
Same weight too.
http://forums.mycotopia.net/contests...s-contest.html (The Great Panaeolus contest)

[nyi]

I'll post pics of the prints, ok? I don't like to talk dump statements, supplement them with sentences like "I'm sure!", or "I know wha I'm talking about..."... that was another part of my experience... I'm lying about many things to myself.
Workman will get a print, or culture, if he'd like, any one will get some, just a PM will do the trick. Anyway, everything, what will be left, will go to fsre.nl.
About the "good first attempt"... I'm just a lucky moron My last successful PC (Lizard king) grew thick and short too... the best fruit was under 10cm (4in) and weighed 24g! They were fruited on spelt/quinoa/ground-flax/ground-millet a very good mix, but easy to contaminate.
I remembered my first attempt with cubensis, it's in the first pic. I used NO lime, and you can see the pinset... but the hole in center of the tray/tub/terrarium is after heavy infection of Trichoderma. It eat up 30% of substrate in 2 days! I got very anxious after this incident. Now I add a bunch of lime and/or CaOH and sterilize the coir. I'll try now to add 2/3 or less of tea spoon of lime to the casing mix and We'll see.
And now a surprise. Do you remember the pans on grain in a jar? When it was colonized for some 2 weeks, I added pasteurized horse manure on the top, left it 3 days and it colonized jet-fast. It look, that leaving the mycelium to decompose the grain adds much "colonization pressure" to the culture. Then I cased it and after about 3 days... you can see these little boys on the picture.

The neck is ~5cm (2in) in diameter. 8 pins in 8hours and in my experience between the first and last pin it take about 36 hours, roughly... we'll see. But with this amount of grain nutrients and substrate height, it'll be great. But I fear, that the little amount of manure will hurt yield. On the other side, the tray, which produced the big fruit is less then 2,5cm (1in) deep. This jar has from 2cm (0,8in) on one side, to 12cm (4,8in) on the other (EDIT: of manure).

[Foster]

awesome Spacecake. What colr did you get when you printed, same as nyi described?

I envy both of you for the tremendous size of your panis. Pan envy I guess Lol.

I really like that somewhat closeup pic of the slightly upturned edge on the one, really nice.

Thanks for sharing nyi and the info Workman and Spacecake. You guys rock!

[spacecake]

Didn't printed those...,sorry...

[nyi]

I don't believe, that growing from prints will yield similar specimens and the one, from which was the print taken. Of course, doing this several times (for example growing 100times from the print of the biggest specimen) will change the genetic suspectability of the strain, at some time branching a new strain from the original strain. But I don't really don't think, that one cycle will do the trick. Cloning is the real deal.

(I'm not used to get answer every hour or so, this is great on this and other big, international forum... long life mycotopia!)

[nyi]

Here are a few new pics. The Jar with colonized grain, pasteurized dung placed on top and cased with the same highly basing casing truited great and the fruits look much more like Pans. cambo. They were isolated from the same plate as the first fruiting tray with dark caps.
The tray is in 3flush, nice... but I wonder, what the potency will be.
Look at the color, it's not brown-gray, but grey as on many other images. This is probably due to different position in the FC relative to my light bulb. I don't know if the artificial 6400K LFC helped, but make some differenced in the terrarium, that's for sure. Anyway it holds temps a little bit up, more or less ideal for pans.
The print on the image is from the first dark capped huge fruits. It's jet-black. I was 90% sure and here you have the result