My Grain Jars.

Shroomhog · 10-14-05, 15:36

This is the way I've been doing my grain jars and I've been having about an 80% success rate. First I pour my grain into a large pot(Penningtons finch food), and cover with about 2 or 3 inches of water. I bring it to a hard boil, Then cover and remove from heat. I let it sit for an hour or so then rinse repeatedly in cold water. I put 2 cups of grain in each jar and use 2 coffee filters, and tin foil. I PC for 75 minutes. When I take the jars out I shake them to loosen up the grains. After the jars cool I inject 5cc's of hydrogen peroxide into each jar and shake again, I let them sit for an hour then inoculate with 2cc's of spore solution. It takes about 5 to7 days for an signs of growth. Any comments on how I can improve on this or something that maybe I should do different.

laborer · 10-14-05, 16:12

Use tyvek instead of coffee filters. Also, I think you can omit the 5cc's peroxide since pc'ing the grain itself should be sufficient. As for the moisture content, if what you do works, stick with it. I myself just simmer 30-40 minutes, rinse well and load jars.

10-14-05, 20:49

No need for the h2o2. Sterilizing at 15 psi for 75 minutes should kill any nasties. Tyvek is probably better to use too, like laborer said. I'd use maybe 4 layers though to keep the grain from drying out. And 5-7 days isn't bad for germination. I have had to wait almost 2 weeks to see germination from multi-spore inoculations. Some are fast some are slower. Liquid Cultures tend to speed it up a little. If it is working for you , don't change anything. If you are looking for faster jar growth, incubation can speed it up by a few days. Usually it just takes as long as its gonna take.

~Hippie3 · 10-15-05, 10:05

what is happening to that lost 20% ?
mold ? bacteria ? both ? stalling ?

Shroomhog · 10-16-05, 08:17

Most of the contam's are of the green variety. As for leaving out the hydrogen I have noticed a big diff in the contaminate rate with out it.

Shroomhog · 10-16-05, 08:19

Also I was going to say that without the hydrogen peroxide the jars seem to show signs of growth a lot quicker.

~Hippie3 · 10-16-05, 08:39

since it's green mold
i'd recommend ditching the coffee filters too
and get tyvek or polyfil or even just seal the jars up.

10-16-05, 09:16

I just use polyfil. As long as things are relatively clean I never get grain contams

sandman · 10-16-05, 10:51

everytime i injected h202 into my jars back in the day i would get a pile of wet spot bacteria.

insight · 10-16-05, 14:04

H202 will stall growth and has found to be uneffective against mold expect cobweb.

~Hippie3 · 10-17-05, 08:15

there's an old tek where the peroxide is injected before inoculation,
it's quickly destroyed by the organics present
and should not adversely impact germination.

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10-14-05, 15:36	#1 (permalink)
Shroomhog Mycophiliac Join Date: Oct 2005 Posts: 43	My Grain Jars. This is the way I've been doing my grain jars and I've been having about an 80% success rate. First I pour my grain into a large pot(Penningtons finch food), and cover with about 2 or 3 inches of water. I bring it to a hard boil, Then cover and remove from heat. I let it sit for an hour or so then rinse repeatedly in cold water. I put 2 cups of grain in each jar and use 2 coffee filters, and tin foil. I PC for 75 minutes. When I take the jars out I shake them to loosen up the grains. After the jars cool I inject 5cc's of hydrogen peroxide into each jar and shake again, I let them sit for an hour then inoculate with 2cc's of spore solution. It takes about 5 to7 days for an signs of growth. Any comments on how I can improve on this or something that maybe I should do different.

10-16-05, 10:51	#9 (permalink)
sandman l̈́͂̏̔ͯ̒͒͊͒̃ͮ̍̍̚ȏ̾̂̉̍́ ͒͛́ Join Date: Apr 1972 Posts: 2,088	everytime i injected h202 into my jars back in the day i would get a pile of wet spot bacteria. __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

10-16-05, 14:04	#10 (permalink)
insight Mycotopiate Join Date: Feb 1970 Posts: 835	H202 will stall growth and has found to be uneffective against mold expect cobweb. __________________ "I create cultures, not those of politics and society, but those for the good of the people" -Myself

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10-14-05, 16:12	#2 (permalink)
laborer Mycophiliac Join Date: Oct 2005 Posts: 59	Use tyvek instead of coffee filters. Also, I think you can omit the 5cc's peroxide since pc'ing the grain itself should be sufficient. As for the moisture content, if what you do works, stick with it. I myself just simmer 30-40 minutes, rinse well and load jars.

10-14-05, 20:49	#3 (permalink)
~freakachino Guest Posts: n/a	No need for the h2o2. Sterilizing at 15 psi for 75 minutes should kill any nasties. Tyvek is probably better to use too, like laborer said. I'd use maybe 4 layers though to keep the grain from drying out. And 5-7 days isn't bad for germination. I have had to wait almost 2 weeks to see germination from multi-spore inoculations. Some are fast some are slower. Liquid Cultures tend to speed it up a little. If it is working for you , don't change anything. If you are looking for faster jar growth, incubation can speed it up by a few days. Usually it just takes as long as its gonna take.

10-15-05, 10:05	#4 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	what is happening to that lost 20% ? mold ? bacteria ? both ? stalling ?

10-16-05, 08:17	#5 (permalink)
Shroomhog Mycophiliac Join Date: Oct 2005 Posts: 43	Most of the contam's are of the green variety. As for leaving out the hydrogen I have noticed a big diff in the contaminate rate with out it.

10-16-05, 08:19	#6 (permalink)
Shroomhog Mycophiliac Join Date: Oct 2005 Posts: 43	Also I was going to say that without the hydrogen peroxide the jars seem to show signs of growth a lot quicker.

10-16-05, 08:39	#7 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	since it's green mold i'd recommend ditching the coffee filters too and get tyvek or polyfil or even just seal the jars up.

10-16-05, 09:16	#8 (permalink)
~thafunkyone Guest Posts: n/a	I just use polyfil. As long as things are relatively clean I never get grain contams

10-17-05, 08:15	#11 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	there's an old tek where the peroxide is injected before inoculation, it's quickly destroyed by the organics present and should not adversely impact germination.

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