substitution of jello mix for agar agar

[valleyofmushies]

well i tried it today -the making of agar agar once again .....this time to help it hold together i used a oatmeal agar agar recipe and while looking at the ingredients i only had oatmeal and jello mix and sterilized water of course so i just mixed it together in a sorted of cutting ration fashion since i only made an eighth of wat it called for it did solidify and i hope it works well i need strain iso bad a yield difference too........ will post updates but heres pic

[ishkaboo]

Don't see a pic.

If it doesn't work u can try cardboard, works for me.

[valleyofmushies]

oohhhh i love ur recycling ideas from ppl here thats a good idea i will try to just to do it oh and i was high when i wrote that post above i just forgot to include the pic my bad will get on it

[hyphaenation]

Jello has loads of sugar-type substances , probably far more than required. As Isk mentioned cardboard works very well. You can search here for info on cardboard isolation. Many good threads.

An easy way to use cardbaord:

- Harvest shroom you want to isolate.
- Take cap off
- Soak strip of cardboard 2 - 3 inches wide and 4 to 6 inches long (hot water if you want)
- After the cardboard is soaked pull of the top and bottom paper exposing the corregated , wavy piece
- Place stalk or piece of stalk on the end of the corregated and roll it up like sushi
- Store in a yogurt container with polyfil breather
- Mist every few days if it looks dry

In 48 hours or close to it will look like this:



Now you can throw the colonized roll in a blender with water and make a clone-slurry. Dump that into a wire screened strainer and let it drain.

Anything you mix this into will make clone substrate. Squeeze any extra water out well. I like to use things like horse manure , leaves , garlic straw chips , coir , more cardboard and may other things.

Probably the easiest way to go is mix the clone-cardboard pulp with your favorite bulk sub mix and squeeze hard with both hands like your making a tight snowball....

Cardboard clone-balls:



Or flatten those balls and they become clone-burgers



Best of luck in any case.

[valleyofmushies]

so it worked kinda...i tried to just put piece of skinned stem in LC and not so good but put one piece on a jello oatmeal mix in a pint jar ...kinda like agar oatmeal but without the agar and well after like a week or less , it pinned out or what ever cause it looked like a drugged out hippie's hair ...so i did wat somebody said somewhere about agar agar and it said do the agar then transfer over to lc and i took two pieces and put in lc and both look good. MY QUESTION is ...is this strain isolation???? since its from a tissue of a mushroom fruit body???the second pic of the fruit body of the clone

[johnnydigital]

Cloning would be the proper term, since you used tissue from a fruit body. It is an 'isolate' in the sense that it will have the same genetics.