sclerota clone from dry tissue

zodd · 05-07-09, 14:51

Swims just got 4 grams dry of philosophers stones through the post and he wants to know weather he can clone these bad boys to make more of them.

He recently cloned some shiitake and a shop bought oyster strain uknown with great results only needed one transfer and there about ready for the lc.

But he knows that it will be much harder to get a clean specimen of sclerota as they are nearly rock solid when dry and this means more fiddling time to try and get into them.

So iv’e come up with a few possible solutions for him

hit it with a hammer first to soften it up a bit

hydrate it in peroxide then clone as normal.

Swim will try both and see how it goes
Any and all advice is appreciated

( |= 0

the_chosen_one · 05-07-09, 15:52

should work, provided the stones are not completely dry. the material in the center may still have some viable cells. trick is whats the middle after it's smashed lol. you may want to use something like a screw driver to split it rather than smash it all together.

good luck and keep us posted!

Doing_Bad · 05-07-09, 16:05

Should be useful experience for other shroomheads if one manage to do that))

mycot · 05-11-09, 00:58

Definately has been done. Although sclerotia may be rockhard one can still create cloneable shavings using a razorblade. Keep us posted.

zodd · 05-11-09, 03:58

well its day three now and no contaminants. peroxide realy works. i decided to brake a small one into three and make them my subjects as the hammer thing sounded messy.
one was simply peroxidated and then transferred over to ma.
the second was hydrated in sterile water for twentyfour hours then peroxidated before transfer.
and the last was bleached bathed in 1/200 percent bleach water and transferred
ive never heard of the last one being done before but three always makes for a better test for some reason.
ow and thanks for the reassurance mycot. this may be a long experiment if i do manage to get viable cells i want to test a claim i heard a while ago. which was the longer you leave sclerota the bigger they become.

has any one heard of this?

DrDee · 05-11-09, 07:51

Quote:

Originally Posted by zodd

i want to test a claim i heard a while ago. which was the longer you leave sclerota the bigger they become. has any one heard of this?

Hi Zodd,
I don't think that's such a wild "claim"...isn't that the way it goes with many living growing things? The longer you let them grow, the bigger they get?
Dee

zodd · 05-15-09, 17:01

well i thought id let you know that this ended in absolute failure. Might try the cardboard tek later this year after i manage to find a new job.

relaly wanted to try my hand at stones and some mex a shrooms. Gutted

thanks for the advice and post you guys.

DrDee · 05-15-09, 18:36

Quote:

Originally Posted by zodd

well i thought id let you know that this ended in absolute failure. Might try the cardboard tek later this year after i manage to find a new job.

relaly wanted to try my hand at stones and some mex a shrooms. Gutted

thanks for the advice and post you guys.

Hey, it was worth a try. Spore prints and syringes for sclerotia strains are readily available...so that's probably the way to go.
D

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05-07-09, 14:51	#1 (permalink)
zodd Maniacal Laughter Mwahaa! Join Date: Jan 2009 Posts: 584	sclerota clone from dry tissue Swims just got 4 grams dry of philosophers stones through the post and he wants to know weather he can clone these bad boys to make more of them. He recently cloned some shiitake and a shop bought oyster strain uknown with great results only needed one transfer and there about ready for the lc. But he knows that it will be much harder to get a clean specimen of sclerota as they are nearly rock solid when dry and this means more fiddling time to try and get into them. So iv’e come up with a few possible solutions for him hit it with a hammer first to soften it up a bit hydrate it in peroxide then clone as normal. Swim will try both and see how it goes Any and all advice is appreciated ( \|= 0 __________________ who would win between a monkey and a dog?

05-07-09, 15:52	#2 (permalink)
the_chosen_one Darth Moderator Join Date: May 2005 Posts: 5,120	should work, provided the stones are not completely dry. the material in the center may still have some viable cells. trick is whats the middle after it's smashed lol. you may want to use something like a screw driver to split it rather than smash it all together. good luck and keep us posted! __________________ "Luck favors the observant." - Workman

05-07-09, 16:05	#3 (permalink)
Doing_Bad Mycophiliac Join Date: Jun 2008 Posts: 13	Should be useful experience for other shroomheads if one manage to do that)) __________________ Is there any forum where entheogens discuss growers?

05-11-09, 03:58	#5 (permalink)
zodd Maniacal Laughter Mwahaa! Join Date: Jan 2009 Posts: 584	well its day three now and no contaminants. peroxide realy works. i decided to brake a small one into three and make them my subjects as the hammer thing sounded messy. one was simply peroxidated and then transferred over to ma. the second was hydrated in sterile water for twentyfour hours then peroxidated before transfer. and the last was bleached bathed in 1/200 percent bleach water and transferred ive never heard of the last one being done before but three always makes for a better test for some reason. ow and thanks for the reassurance mycot. this may be a long experiment if i do manage to get viable cells i want to test a claim i heard a while ago. which was the longer you leave sclerota the bigger they become. has any one heard of this? __________________ who would win between a monkey and a dog?

05-11-09, 00:58	#4 (permalink)
mycot Mycotopiate Join Date: May 2006 Posts: 359	Definately has been done. Although sclerotia may be rockhard one can still create cloneable shavings using a razorblade. Keep us posted.

05-15-09, 17:01	#7 (permalink)
zodd Maniacal Laughter Mwahaa! Join Date: Jan 2009 Posts: 584	well i thought id let you know that this ended in absolute failure. Might try the cardboard tek later this year after i manage to find a new job. relaly wanted to try my hand at stones and some mex a shrooms. Gutted thanks for the advice and post you guys. __________________ who would win between a monkey and a dog?

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