Liquid culture storage life

RedOctober · 05-20-09, 19:25

If you put a fully developed LC in the fridge, I'm curious as to how long it will keep and be useful for (yes I know you shouldn't end a sentence with a preposition)?

I have a clone isolate I'd like to keep for future use.

whatisntseen · 05-20-09, 20:03

can't tell you for sure, but I've heard that LC's in the fridge can last a few months. If anything, I'm sure you could innoc other LCs with it and increase its lifespan for a little longer.

EDIT: If you decide to extend its life like that, I suggest doing a test jar to make sure it's a clean and healthy lc.

whatchamacallit · 05-20-09, 20:05

SWIM has seen an LC stay good in the fridge for a year before, but not sure of the exact mixture that was used and simple karo LC's may not last quite as long once the sugars are used up.

Shroomette · 05-20-09, 20:12

Liquid cultures can be stored in a fridge for 6-8 months (or longer). Some add a little H202 (approx 1-3cc) at this point since the mycelium is able to handle it, this can help prevent contamination. The best LC that would work is the Honey/karo LC imho.

buteo · 05-20-09, 20:32

haha i dont even put mine in the fridge. I have a culture that is 4 months old and its been sittin out. it still works

i still use it and have some nice tubs going with it right now

. Thats the Irish Lion 7.5% honey/karo mix.

Shromadon · 05-20-09, 21:10

I had an Lc that was kept in a room with air-conditioning for a month, then I forgot about it in a room that spiked to high 80's for about 4 months, found it, and moved out of that place. 2 months later and was able to make 120 perfect jars

So I imagine if you keep it in a fridge you can go well over a year.

wayback · 05-21-09, 12:39

I've gone 3-4 months with few problems, however, I have also heard they can last longer. On the other hand, I seal my jar sitting straight up in the fridge in a zip lock back and hold the culture up to the light from time to time to look for discolorations. It has been my experience when any discoloration, or flecks of other than white material occur, then it's best to toss that jar. Remember, contams cost time.

Shromadon · 05-21-09, 17:36

Careful about throwing away LCs, do some test jars first. I've never had a nice looking LC. When you hold it up to a light and look at it the thicker groupings of myc will look very dark like contam, buts just shadows. It takes ALOT of effort to position multiple lights in such away that you perfectly light it up to see the whiteness.

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05-20-09, 19:25	#1 (permalink)
RedOctober Mycophage Join Date: Oct 2005 Posts: 193	Liquid culture storage life If you put a fully developed LC in the fridge, I'm curious as to how long it will keep and be useful for (yes I know you shouldn't end a sentence with a preposition)? I have a clone isolate I'd like to keep for future use.

05-20-09, 20:12	#4 (permalink)
Shroomette Fairy Princess Join Date: May 2008 Posts: 2,935	Liquid cultures can be stored in a fridge for 6-8 months (or longer). Some add a little H202 (approx 1-3cc) at this point since the mycelium is able to handle it, this can help prevent contamination. The best LC that would work is the Honey/karo LC imho. __________________ "If I'm not seen in this world.I'm lost in my own lil world"!!!

05-20-09, 20:32	#5 (permalink)
buteo Imbicilzzzz Join Date: Oct 2008 Posts: 1,355	haha i dont even put mine in the fridge. I have a culture that is 4 months old and its been sittin out. it still works i still use it and have some nice tubs going with it right now . Thats the Irish Lion 7.5% honey/karo mix. __________________ Im only what i think i am

05-20-09, 21:10	#6 (permalink)
Shromadon Comfortably Numb Join Date: Feb 2007 Posts: 156	I had an Lc that was kept in a room with air-conditioning for a month, then I forgot about it in a room that spiked to high 80's for about 4 months, found it, and moved out of that place. 2 months later and was able to make 120 perfect jars So I imagine if you keep it in a fridge you can go well over a year. __________________ Trippin through life

05-20-09, 20:03	#2 (permalink)
whatisntseen Behind the Door Join Date: Apr 2009 Posts: 70	can't tell you for sure, but I've heard that LC's in the fridge can last a few months. If anything, I'm sure you could innoc other LCs with it and increase its lifespan for a little longer. EDIT: If you decide to extend its life like that, I suggest doing a test jar to make sure it's a clean and healthy lc.

05-20-09, 20:05	#3 (permalink)
whatchamacallit Deadhead Join Date: Jan 2007 Posts: 1,168	SWIM has seen an LC stay good in the fridge for a year before, but not sure of the exact mixture that was used and simple karo LC's may not last quite as long once the sugars are used up.

05-21-09, 12:39	#7 (permalink)
wayback Mycotopiate Join Date: Jan 1973 Posts: 329	I've gone 3-4 months with few problems, however, I have also heard they can last longer. On the other hand, I seal my jar sitting straight up in the fridge in a zip lock back and hold the culture up to the light from time to time to look for discolorations. It has been my experience when any discoloration, or flecks of other than white material occur, then it's best to toss that jar. Remember, contams cost time.

05-21-09, 17:36	#8 (permalink)
Shromadon Comfortably Numb Join Date: Feb 2007 Posts: 156	Careful about throwing away LCs, do some test jars first. I've never had a nice looking LC. When you hold it up to a light and look at it the thicker groupings of myc will look very dark like contam, buts just shadows. It takes ALOT of effort to position multiple lights in such away that you perfectly light it up to see the whiteness. __________________ Trippin through life

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