P. bohemica culture

nyi · 05-22-09, 06:27

Hello. Few week ago I got a print of P. bohemica from my friend from Czech republic. I tried to germinate it a few times, but without success. I sent the print to another member in hope, he will manage to do it.

I looked on the last agar plate after a week of ingoring it. The spores germinated after 2-3 week, I'm not sure, when. This is the culture and I'm 99% sure it's bohemica. So it's possible and it's on a good way to get this rare specie into circulation.

Om shanti · 05-22-09, 11:40

Congratulations, Nyi. Seems like you grow are growing a ton of exotics at the same time?! ;-) As far as I remember from some table of psilocybin content in mushrooms it's a strong little mushroom. How are you going to grow it? First to cakes or grain, and then.. is it a dung or woodlover?

nyi · 05-22-09, 12:08

P. bohemica is a wood lover. It grows on declaying wood mainly, but still rarely in Czech republic. I was introduced by this species to the mysteries of mushrooms (but I had some DMT and one LSD session before, but mushrooms have a special place in my heart).

I never had semilanceata, but from reports I've read this mushroom seams very close to it in subjective effects. It had reasonably good taste, is euphoric and there is what I call "The healing presence", which I never encountered with cubies. My recent first trip on pans seamed from the start similar, but after some hour went in completely different way. Very interesting specie.

I'm still trying and gaining basic knowledge. I'll try my own method as with azures and subaeruginosa: I'll transfer the culture to a jar with agar at bottom and let the mycelium to colonize it 100% of agar. After that I'll throw some sterilized wood sticks in the jar. When they colonize, more wood sticks will be added, this time only bleach/hot water soaked. With these more wood will be inoculated.

I do this with wood lovers, because I had bad luck with grain spawn and ever attempt contaminated because the grain rotted, or mold germinated on it. Of course, I'll transfer a piece of the mycelium to grain and I'll try spawn this way again, but only to sterilized wood inside a jar, not a tub, or anything where contamination is a possibility.

Om shanti · 05-22-09, 15:12

Interesting, Nyi! Good luck and do continue to share your progress!

As you can see I how absolutely no idea about this species (or any woodlover actually), but it's great to read about! ;-)

nyi · 05-22-09, 19:17

Most cultivated woodlover are said to be 3 times as potent as cubes, these are: P. azurescens, P. cyanescens and P. subaeruginosa; all are closely related. The down side of these are that they have sometimes unpleasant side effects, like heavy body load.
P. bohemica is realted to P. moravica, which is uncommon too and is mostly distributed in a east part of Czech republic called Morava. Bohemica is only about 1,5-2 times stronger then Cubensis, but with very little body load, it promotes the "mind trip" similar to Pans, but maybe not so visual, anyway very emotional.
Today the culture grown a few milimeters, I'm happy, but I'm getting a little bit paranoid if it's really bohemica and not some strange contamination. Looks pretty the same as cubensis. I inoculated it on my crazy alfalfa/rice/limestone/wheat_flour/dung/maltose/dextrose/yeast/riboflavin agar. It was the only agar I had... one of my experiments... and I'm surprised, because nothing survived on this agar, except for yeast contamination.

spacecake · 05-22-09, 22:07

Quote:

Originally Posted by nyi

I never had semilanceata, but from reports I've read this mushroom seams very close to it in subjective effects. It had reasonably good taste, is euphoric and there is what I call "The healing presence", which I never encountered with cubies. .

Yep,liberty's are somewhat special compared to cubies.
Had the most interesting trips on those,but sometimes a little scary too.

nyi · 05-28-09, 03:13

Psilocybe coprinifacies and Psilocybe mairei are synonymous for Psilocybe bohemica. This was unknown to me till now. My source is Czech wikipedia:
http://cs.wikipedia.org/wiki/Lysohlávka_česká.
The dish was almost fully colonized yesterday, I'll post a few pictures this evening.

Awestruck · 05-29-09, 01:07

I love hearing about the more exotic species; I think that it is great that you are trying your hand at such a rare specimen. I'll be watching to see how it goes. Happy growing!

nyi · 05-29-09, 03:27

Thank you, but I'd say I'd better keep my hands off many projects. But this is something special.

I was wondering why this species is so rare. Maybe because the spores are hard to germinate. After germination the mycelium is fast and hopefully fruits easily in the right conditions.

nyi · 05-29-09, 03:36

Picture from last night. The 'banding' or circles around the inoculation points is probably due to the crazy agar recipe I used. Because the place is 99% colonized, I'll transfer the culture to new plates and into test tubes this weekend.

I've done some sterile work yesterday... it was again time consuming and I don't have much time, so hopefully I'll get it all done ASAP.

Awestruck · 06-01-09, 22:04

That mycelium looks dense and healthy. I like the texture in the pic. Very nice characteristics.

nyi · 06-02-09, 02:02

It looks better in reality! If I would have a better camera at hand... mine is broken and I don't have the motivation to get it repaired, other then taking sharp pics of my grow... but that's not something what is really motivating me to spend my precious time in visiting some store and paying money for battery charging circuit replacement, instead of investing the time to my hobby and my girlfriend and spending money on them both.

Sorry guys, your one brief look on a sharp photo of mine isn't important for me

Anyway... there is still the possibility for you to look at it yourself while holding it in your hands in you kitchen lab... just drop me a message.

nyi · 06-02-09, 02:08

Oh and one thing. The petri dish with the mycelium has a plenty of air exchange. After transfers, I'll wrap the some dishes into parafilm and some into PE foil and we'll se what dense mycelium looks like. The woodlovers tend to produce very dense mycelium, if air exchange is cut down.

Awestruck · 06-02-09, 12:50

Quote:

Originally Posted by nyi

Sorry guys, your one brief look on a sharp photo of mine isn't important for me

Ha, that's pretty funny. I have to agree with you on that one. There are much better things to spend money on.

nyi · 06-02-09, 15:30

The best things on these species is that, besides the growing on store bought grain, you have to do some physical work! For woodlovers you have to visit our garden and dress-up some of your trees, cut them to chips and bleach them. For dung loving species you have to find some good manure, gather it, leach it, dry it, crumble it and pasteurize it. Similarly for straw.

In my profession, you need to have some manual hobby.

spacecake · 06-03-09, 01:05

it sure is an addictive hobby...!

nyi · 06-03-09, 02:07

Yes... I frequently find myself culturing repeatedly, not able to stop... and I have to increase the dose to get to the same level. Soon I'll find myself culturing molds, because they're the easiest and cheapest crap

[Home]	[The Vaults]	[Glossary]	[Sponsors]	[Affiliates]
[Search]	Today's Posts	Mark Forums Read	[Register]	[Activate]	[Resend Email]

05-22-09, 06:27	#1 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	P. bohemica culture Hello. Few week ago I got a print of P. bohemica from my friend from Czech republic. I tried to germinate it a few times, but without success. I sent the print to another member in hope, he will manage to do it. I looked on the last agar plate after a week of ingoring it. The spores germinated after 2-3 week, I'm not sure, when. This is the culture and I'm 99% sure it's bohemica. So it's possible and it's on a good way to get this rare specie into circulation. Attached Thumbnails __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

05-22-09, 11:40	#2 (permalink)
Om shanti S.W.I.M. in H.POO Join Date: Jan 2008 Posts: 1,297	Congratulations, Nyi. Seems like you grow are growing a ton of exotics at the same time?! ;-) As far as I remember from some table of psilocybin content in mushrooms it's a strong little mushroom. How are you going to grow it? First to cakes or grain, and then.. is it a dung or woodlover? __________________ The most important thing is to find out what is the most important thing.-S. Suzuki

05-22-09, 12:08	#3 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	P. bohemica is a wood lover. It grows on declaying wood mainly, but still rarely in Czech republic. I was introduced by this species to the mysteries of mushrooms (but I had some DMT and one LSD session before, but mushrooms have a special place in my heart). I never had semilanceata, but from reports I've read this mushroom seams very close to it in subjective effects. It had reasonably good taste, is euphoric and there is what I call "The healing presence", which I never encountered with cubies. My recent first trip on pans seamed from the start similar, but after some hour went in completely different way. Very interesting specie. I'm still trying and gaining basic knowledge. I'll try my own method as with azures and subaeruginosa: I'll transfer the culture to a jar with agar at bottom and let the mycelium to colonize it 100% of agar. After that I'll throw some sterilized wood sticks in the jar. When they colonize, more wood sticks will be added, this time only bleach/hot water soaked. With these more wood will be inoculated. I do this with wood lovers, because I had bad luck with grain spawn and ever attempt contaminated because the grain rotted, or mold germinated on it. Of course, I'll transfer a piece of the mycelium to grain and I'll try spawn this way again, but only to sterilized wood inside a jar, not a tub, or anything where contamination is a possibility. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

05-22-09, 15:12	#4 (permalink)
Om shanti S.W.I.M. in H.POO Join Date: Jan 2008 Posts: 1,297	Interesting, Nyi! Good luck and do continue to share your progress! As you can see I how absolutely no idea about this species (or any woodlover actually), but it's great to read about! ;-) __________________ The most important thing is to find out what is the most important thing.-S. Suzuki

05-22-09, 19:17	#5 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Most cultivated woodlover are said to be 3 times as potent as cubes, these are: P. azurescens, P. cyanescens and P. subaeruginosa; all are closely related. The down side of these are that they have sometimes unpleasant side effects, like heavy body load. P. bohemica is realted to P. moravica, which is uncommon too and is mostly distributed in a east part of Czech republic called Morava. Bohemica is only about 1,5-2 times stronger then Cubensis, but with very little body load, it promotes the "mind trip" similar to Pans, but maybe not so visual, anyway very emotional. Today the culture grown a few milimeters, I'm happy, but I'm getting a little bit paranoid if it's really bohemica and not some strange contamination. Looks pretty the same as cubensis. I inoculated it on my crazy alfalfa/rice/limestone/wheat_flour/dung/maltose/dextrose/yeast/riboflavin agar. It was the only agar I had... one of my experiments... and I'm surprised, because nothing survived on this agar, except for yeast contamination. Attached Thumbnails __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

05-28-09, 03:13	#7 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Psilocybe coprinifacies and Psilocybe mairei are synonymous for Psilocybe bohemica. This was unknown to me till now. My source is Czech wikipedia: http://cs.wikipedia.org/wiki/Lysohlávka_česká. The dish was almost fully colonized yesterday, I'll post a few pictures this evening. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

05-29-09, 01:07	#8 (permalink)
Awestruck Mycophage Join Date: May 2009 Posts: 127	I love hearing about the more exotic species; I think that it is great that you are trying your hand at such a rare specimen. I'll be watching to see how it goes. Happy growing!

05-29-09, 03:27	#9 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Thank you, but I'd say I'd better keep my hands off many projects. But this is something special. I was wondering why this species is so rare. Maybe because the spores are hard to germinate. After germination the mycelium is fast and hopefully fruits easily in the right conditions. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

05-29-09, 03:36	#10 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Picture from last night. The 'banding' or circles around the inoculation points is probably due to the crazy agar recipe I used. Because the place is 99% colonized, I'll transfer the culture to new plates and into test tubes this weekend. I've done some sterile work yesterday... it was again time consuming and I don't have much time, so hopefully I'll get it all done ASAP. Attached Thumbnails __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

06-01-09, 22:04	#11 (permalink)
Awestruck Mycophage Join Date: May 2009 Posts: 127	That mycelium looks dense and healthy. I like the texture in the pic. Very nice characteristics.

06-02-09, 02:02	#12 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	It looks better in reality! If I would have a better camera at hand... mine is broken and I don't have the motivation to get it repaired, other then taking sharp pics of my grow... but that's not something what is really motivating me to spend my precious time in visiting some store and paying money for battery charging circuit replacement, instead of investing the time to my hobby and my girlfriend and spending money on them both. Sorry guys, your one brief look on a sharp photo of mine isn't important for me Anyway... there is still the possibility for you to look at it yourself while holding it in your hands in you kitchen lab... just drop me a message. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

06-02-09, 02:08	#13 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Oh and one thing. The petri dish with the mycelium has a plenty of air exchange. After transfers, I'll wrap the some dishes into parafilm and some into PE foil and we'll se what dense mycelium looks like. The woodlovers tend to produce very dense mycelium, if air exchange is cut down. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

06-02-09, 15:30	#15 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	The best things on these species is that, besides the growing on store bought grain, you have to do some physical work! For woodlovers you have to visit our garden and dress-up some of your trees, cut them to chips and bleach them. For dung loving species you have to find some good manure, gather it, leach it, dry it, crumble it and pasteurize it. Similarly for straw. In my profession, you need to have some manual hobby. __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

06-03-09, 01:05	#16 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 4,734	it sure is an addictive hobby...! __________________ "As a child, i could walk on the ceiling. I'd butterfly up on the walls"

06-03-09, 02:07	#17 (permalink)
nyi Mycophage Join Date: Dec 2008 Posts: 104	Yes... I frequently find myself culturing repeatedly, not able to stop... and I have to increase the dose to get to the same level. Soon I'll find myself culturing molds, because they're the easiest and cheapest crap __________________ We're animals... nature just decided, that the next global disaster shouldn't be an big fat asteroid... it should be an animal.

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode