antibiotics on/in agar?

[Mushrooms201]

I had recieved an number of prints in trades that I cannot get a clean culture off of. I was wondering how you all handle this problem.

A site I order off of has: would any of these work?

CIM-89073 ANTIBIOTIC SENSITIVITY DISCS, AMPICILLIN, 10mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89079 ANTIBIOTIC SENSITIVITY DISCS, ERYTHROMYCIN, 15mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89081 ANTIBIOTIC SENSITIVITY DISCS, KANAMYCIN, 30mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89084 ANTIBIOTIC SENSITIVITY DISCS, NOVOBIOCIN, 30mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89086 ANTIBIOTIC SENSITIVITY DISCS, PENICILLIN G, 10mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89088 ANTIBIOTIC SENSITIVITY DISCS, STREPTOMYCIN, 10mcg, 50/VIAL






List Price: US$6.50
Your Price: US$6.50


CIM-89089 ANTIBIOTIC SENSITIVITY DISCS, TETRACYCLINE, 30mcg, 50/VIAL

[ggod]

Gentamiacin (sp?) is what is generally used. Is it not heat sensitive therefor it can be autoclaved.

[joystik]

check out sporeworks. They have the antibiotics your looking for.(Gentamicin)

[sangraal]

Remember, unless your primary problem with cultures is a bacterial infection (not something typically easy to determine), adding an antibiotic to your agar won't do much good. Antibiotics won't do anything for trich or other competing contaminants. I've used Malt Yeast Agar w/ antibiotic for about a year now- still get the mean green, or black... now and then.

But if someone could ever manage to create a selective fungicide, basically taking out anything beyond the higher Basidiomycetes, well... that would be the Holy Grail of mycology. Maybe, hopefully, some day...

[trips6928]

Originally Posted by sangraal

But if someone could ever manage to create a selective fungicide, basically taking out anything beyond the higher Basidiomycetes, well... that would be the Holy Grail of mycology. Maybe, hopefully, some day...

If one could program a microscope to scan agar for foreign beings, other than your spores or mycelium, and zap them out of existence with a laser. That would be the way to go..Or am I in the Twilight Zone?

[Mushrooms201]

Great idea. if we can do it with cancer why not shrooms...