Agar pouring - thinner is better?

Coughing_Anon · 06-03-09, 12:09

I did my first agar work recently, and I noticed that the myc only seems to colonize the top layer of agar.
Would it be wise to pour my agar as thin as possible? It seems like it would save on agar and have little effect.

johnnydigital · 06-03-09, 12:23

How thick do you pour it?

It does no harm to pour it thin. I just barely cover the surface, then stop pouring.

teesus · 06-03-09, 12:34

i had agar wich was very very thin and it dryed out before the plate colonized completly. but mabe if i had sealed the plate better it wouldn't been a problem. i like the agar a bit thicker so when i cut out sections the wedges are more solid and easyer to pic up with a needle for me

johnnydigital · 06-03-09, 12:40

Indeed, thinner will dry out sooner if stored improperly. Really depends on what one plans to do with it. If one is just trying to isolate a strain, thin should be fine. If one is trying to preserve a strain for storage, might want to pour it a bit thicker. Make sense?

Hippie3 · 06-03-09, 13:44

1/8th of an inch or so
works well enough for me.

Awestruck · 06-03-09, 16:21

Quote:

Originally Posted by Hippie3

1/8th of an inch or so
works well enough for me.

I pour just over 1/8 inch as well.

Coughing_Anon · 06-03-09, 16:36

Thank you all very much for your responses.
I didn't take the moisture content into account. I will keep that in mind.

Thin for isolation, thick for storage.

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06-03-09, 12:09	#1 (permalink)
Coughing_Anon Mycophage Join Date: Jun 2008 Posts: 218	Agar pouring - thinner is better? I did my first agar work recently, and I noticed that the myc only seems to colonize the top layer of agar. Would it be wise to pour my agar as thin as possible? It seems like it would save on agar and have little effect.

06-03-09, 13:44	#5 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	1/8th of an inch or so works well enough for me. __________________ visit dial-a-hippie .

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06-03-09, 12:23	#2 (permalink)
johnnydigital Former Member Join Date: Mar 2009 Posts: 713	How thick do you pour it? It does no harm to pour it thin. I just barely cover the surface, then stop pouring.

06-03-09, 12:34	#3 (permalink)
teesus alzheimer Join Date: Nov 2007 Posts: 482	i had agar wich was very very thin and it dryed out before the plate colonized completly. but mabe if i had sealed the plate better it wouldn't been a problem. i like the agar a bit thicker so when i cut out sections the wedges are more solid and easyer to pic up with a needle for me

06-03-09, 12:40	#4 (permalink)
johnnydigital Former Member Join Date: Mar 2009 Posts: 713	Indeed, thinner will dry out sooner if stored improperly. Really depends on what one plans to do with it. If one is just trying to isolate a strain, thin should be fine. If one is trying to preserve a strain for storage, might want to pour it a bit thicker. Make sense?

06-03-09, 16:36	#7 (permalink)
Coughing_Anon Mycophage Join Date: Jun 2008 Posts: 218	Thank you all very much for your responses. I didn't take the moisture content into account. I will keep that in mind. Thin for isolation, thick for storage.