grain liquid culture question

[nicechrisman]

So here's the idea.

add sterile h2o to my fully colonized rye jar, shake it around to stip some of the outer mycelium layer, suck the mycelium laden h20 back out, and use to inoculate next set of rye jars.

Here's my question-most folks I've heard of doing this procedure will let the jar sit for a bit to recover mycelium before using the jar to spawn to bulk. Trouble is I'm a little pressed for time to have this batch ready in time for certain summer events. I'm wondering if anyone has experience with this procedure, and who has had success with just using the "stripped" ryegrain to spawn immediately. It would seem to me that th grain would still be colonized enough on the inside, that it would still fight of contams fairly well. I'm spawning to a coco coir, coffee, verm, gypsum substrate. I'm figuring on 5 qts rye to one block coir, 1 qt each coffee and verm. Mind you only one of the rye qts would be the "stripped" one. This is in a "monotub" setup in a platic bin.

Or would I be better off just setting that jar to the side to re colonize, and only using 4 qts rye to spawn. I'm just worried that would slow down my spawn run. I have about a month till I need them ready, the rye jars are 95-100% colonized at this point.

Thanks in advance for any assistance.

[cap]

coir isnt the best choice for sub ime but to each his own carefuoll adding the coffee to the trich laden coco! if u are pressed for spawn right now wait till u have more to try this or use a traditional tek if u can!

as for the lc tek i think its a great idea but i would NOT let the water sit stagnant for 24hrs just the way u describe, inject, shake up/off the loose myc strands, pool the water in 1 corner of jar, insert needle again, draw the water up with as many mycelial strands as u can muster, rinse and repeat
seriously though let it recover after that.
i highly doubt that jar is gonna like the inpromptu baptism

good luck

Originally Posted by nicechrisman

So here's the idea.

add sterile h2o to my fully colonized rye jar, shake it around to stip some of the outer mycelium layer, suck the mycelium laden h20 back out, and use to inoculate next set of rye jars.

Here's my question-most folks I've heard of doing this procedure will let the jar sit for a bit to recover mycelium before using the jar to spawn to bulk. Trouble is I'm a little pressed for time to have this batch ready in time for certain summer events. I'm wondering if anyone has experience with this procedure, and who has had success with just using the "stripped" ryegrain to spawn immediately. It would seem to me that th grain would still be colonized enough on the inside, that it would still fight of contams fairly well. I'm spawning to a coco coir, coffee, verm, gypsum substrate. I'm figuring on 5 qts rye to one block coir, 1 qt each coffee and verm. Mind you only one of the rye qts would be the "stripped" one. This is in a "monotub" setup in a platic bin.

Or would I be better off just setting that jar to the side to re colonize, and only using 4 qts rye to spawn. I'm just worried that would slow down my spawn run. I have about a month till I need them ready, the rye jars are 95-100% colonized at this point.

Thanks in advance for any assistance.

[nicechrisman]

Thanks for the response. No offense, I'm not really looking for a critique of my bulk substrate. This isn't my first time with coir, and I've had great success as have many others with it(pretty common accepted procedure on other forums). Also, you must have misunderstood what I meant (maybe I wasn't very clear) I'm not planning on leaving the h20 in the jar for 24 hrs. I'm planning on swirling it around and sucking in right back out. My question was just whether I can use the grain to spawn right away after, or if I should let it re colonize before spawning.

Thanks for your response.

[cap]

Originally Posted by nicechrisman

Thanks for the response. No offense, I'm not really looking for a critique of my bulk substrate. This isn't my first time with coir, and I've had great success as have many others with it(pretty common accepted procedure on other forums). Also, you must have misunderstood what I meant (maybe I wasn't very clear) I'm not planning on leaving the h20 in the jar for 24 hrs. I'm planning on swirling it around and sucking in right back out. My question was just whether I can use the grain to spawn right away after, or if I should let it re colonize before spawning.

Thanks for your response.

right, well,
i answered your q.
also gave you a suggestion based on experience
followed directly by a warning that said to each his own--
meaning its not my project to critique.
i, too, reassured you,
that letting it soak for 24hrs is the wrong technique.
imo. you wouldnt have mentioned it if it was irrelevant.
youre welcome.

[stimpy]

coir/coffee/gypsum kicks a lot of ass. I wouldn't use the jar u used to make the grain LC with as spawn. Dont risk contamming a hole tub because you wanted to save a jar of used up spawn. In the future, dont even use quarts for the grain LC's just use half or 1/4 pints it will give you plenty of inoculant for a tub worth of spawn jars.

[nicechrisman]

Thanks for the opinions cap, I do appreciate your opinion. I still don't know where you're getting the 24 hour soak out of what I said. I didn't ever say that. I didn't mean to get off on the wrong foot with you, or offend you. I apologize if I did. Judging by your avatar, we're probably kinda kindred spirits, my friend.

yeah stimpy, you're probably right about not risking using that jar to spawn. That's gonna put me at 4 qts of spawn to a brick of coir, which is less than the 5 that I would prefer. I'm just concerned that's gonna slow me down a little though. I've got about just over a month to have the fruits ready. I think I may just skip this idea entirly and just use all the jars for spawn. Trouble is, I also really want to have another batch ready in about 2 months from now. Might be cutting it a little close with multispore germination...

I know what you're saying about using smaller jars for grain lc though. I wasn't really planning on it when I started the batch. It's gonna be a pain to try to suck the LC out, because the bottom of the jar is deeper than the length of most needles.

And yeah, coir coffee gypsum does kick ass!

[cap]

dude i just reread your post im an asshole for thinking u meant a long soak sorry i really am coming off like a dick here! haha sorry i thought u were too!
wooo! thank goodness i got weed tonite or id still be pissy! hope the relevant info helped. i think i was still half asleep when i read that cuz i read it wrong and deserved the response i got for sure. shake it ez

[mydarling]

i agree on not using that particular jar for spawning. might be best to just go with the remaining 4 qts. perhaps you could decrease the amounts of the remaining substrate materials to maintain a high spawn:substrate ratio - for example, use only 2/3 brick of coir and use a slightly smaller tub so that it doesn't get too thin? IMO, though, 4 qts isn't that much less than 5 qts and it might not make a huge difference. hard to say! how many qts does a brick of coir make.... IIRC it's around 13-14 qts? 4:13 and 5:13 are pretty close in ratio, so it might not take that much longer with just the 4 qts.

[cap]

Originally Posted by mydarling

perhaps you could decrease the amounts of the remaining substrate materials to maintain a high spawn:substrate ratio

i was thinking the exact same thing

[nicechrisman]

Yeah, for sure that's probably the best idea. I remember thinking of it, and thinking that then I would have to sacrifice a tub to cut holes to match the substrate level. But really, who gives a shit-they're cheap as hell. Good thinking, guys. Thanks.

[nicechrisman]

So I ended up sacrificing the jar to make grain LC from. Instead of doing one tub with 5 qts of spawn, I did two 5 gal buckets with 2 qts spawn and 4 qts of coir mix each. Should colonize and fruit pretty quick I figure.

I'm still gonna use that jar. I'm gonna do an ultra un sterile outdoor monobucket with it. We'll see how it does LOL. Never tried outdoor grow before. I hear that outdoor is A LOT more contam resistant. It's worth a shot anyways, I figure.

Thanks for the help and feedback everyone!