New cloning idea

[M&M420]

Just got a really good idea and figured I'd post.In theory this would allow someone to clone in open air to a small amount of grain without contams.Now your thinking how might this be possible?The key is cardboard!I've worked with cardboard quite a bit in open air now without contams the entire time.So you would prep you grain however works best for you and same applies for a piece of cardboard slightly bigger than a half pint.Fill the jar halfway(Faster colonization) and place the piece of carboard over the wbs,close er up,and pressure cook.Then once cool just choose a clone specimen and toss it in the jar.The cardboard should keep the grain from being infected or so I think.Mycelium would then spread to colonize the grain which can in turn be g2g for more spawn.I'm going to give this a try tommorow with two jars.One filled 3/4 with a layer of cardboard either side of clone and one 1/2 with just 1 piece of cardboard.Only problem I see is not being able to shake the jar after cooking.I plan to just let them cool completly in the pc to minimize pooling.If this works it could make cloning conviently easy for anyone with enough experience to prep grain!

[Shroomette]

Good Luck! M&M420. I can't wait to see the results of your experiment. So, I'll be taking a seat for this one.

[Soliver]

I've never used cardboard, but would the shroom mycelium just jump from the tissue
and onto the cardboard? Seems to me it'd just sit there and dry up? Either way,
an interesting theory if it works. Take lots of pics

BTW - how would you then remove the cardboard and tissue sample later, without
infecting the grain?

interesting...



soliver

[M&M420]

Yeah cardboard works excellent for cloning.I originally got the idea from hyph's cardboard cloning machine thread.As far as drying out that is normally a problem with cardboard but since it'll be confined in the jar it should stay moist enough to colonize.Although that is the reason why I'm putting and extra piece of cardboard over the tissue in one jar.Removing the cardboard(optional) and the tissue should be as simple as using a pair of alcohol tweezers to pull it out in a glovebox.The colonized grain should be handled under normal sterile procedure while going g2g from there on out of course.Another thing that came to mind after thinking this through is that the cardboard's nutritional value may change once being cooked with the grain somehow.If this happens then it will be defeating the purpose and making it susceptible to contamination.I'll be sure to take pics later when I get around to it.

[Soliver]

Originally Posted by M&M420

Removing the cardboard(optional) and the tissue should be as simple as using a pair of alcohol tweezers to pull it out in a glovebox.The colonized grain should be handled under normal sterile procedure while going g2g from there on out of course.

But doesn't using a glovebox neutralize the point of the TEK? Cloning is
pretty simple in a glovebox already, without cardboard, so why the extra
steps with cardboard?



soliver

[M&M420]

Very true.Guess I just want to see if I can get colonized wbs in open air.If it does indeed work why not just skip the whole tyvek sleeve and h202 procedure though.It may be a pretty simple and easy routine but the glovebox is the PITA.Now if I had a flowhood that would be another story.

[plantman21]

I would like to Know if this work just to add one more tek to my data base. Hope alls goes well.
Plantman

[Soliver]

Originally Posted by M&M420

Now if I had a flowhood that would be another story.

Ah yes, the grail of mycology..



soliver