colonized grain to lc?

Renz · 07-02-09, 22:07

hello, I couldn't find information about this but I might have skipped it.
Is it possible to mix colonized grain with water, suck it in the syringe, and
inoculate a few LCs with it? has anyone ever tried this? the reason I ask
is because I have some left over colonized PE that took two months to
colonize due to old spores!

peace

Indonesia · 07-02-09, 23:06

if it was clean,yes. try for the tiniest peice to cut down chances of contam. foaf just takes one kernel to agar first and transfers, then goes to lc any time after that when done isolating.

stimpy · 07-02-09, 23:53

http://forums.mycotopia.net/liquid-c...k-revised.html (-Fahtty Isolation/Mycelium syringe tek- *REVISED*)

Soliver · 07-02-09, 23:55

Sure - works great as long as you stay clean. Oftentimes I'll birth PF jars, squirt a
syringe of sterile water into the empty jar, swirl it around in the myc at the bottom
of the jar, and suck it back up. If you use it quickly, it'll colonize whatever you
want like a champ... I use a ghetto glovebox when doing this - gotta stay clean!

soliver

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07-02-09, 22:07	#1 (permalink)
Renz Mycotopiate Join Date: Oct 2008 Posts: 265	colonized grain to lc? hello, I couldn't find information about this but I might have skipped it. Is it possible to mix colonized grain with water, suck it in the syringe, and inoculate a few LCs with it? has anyone ever tried this? the reason I ask is because I have some left over colonized PE that took two months to colonize due to old spores! peace

07-02-09, 23:53	#3 (permalink)
stimpy rawr Join Date: Nov 2008 Posts: 411	http://forums.mycotopia.net/liquid-c...k-revised.html (-Fahtty Isolation/Mycelium syringe tek- REVISED) __________________ RAWWR

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07-02-09, 23:06	#2 (permalink)
Indonesia Mycotopiate Join Date: Nov 2007 Posts: 217	if it was clean,yes. try for the tiniest peice to cut down chances of contam. foaf just takes one kernel to agar first and transfers, then goes to lc any time after that when done isolating.

07-02-09, 23:55	#4 (permalink)
Soliver Deviant Join Date: Feb 2005 Posts: 1,784	Sure - works great as long as you stay clean. Oftentimes I'll birth PF jars, squirt a syringe of sterile water into the empty jar, swirl it around in the myc at the bottom of the jar, and suck it back up. If you use it quickly, it'll colonize whatever you want like a champ... I use a ghetto glovebox when doing this - gotta stay clean! soliver