Quart Jar Invitro TEK

p2k1 · 07-06-09, 12:17

Hey everyone Ive got a large amount of rye grain spawn colinized thats getting spawned outside but thought Id give a little experiment a try. Ive seen this online and have been searching but can't find exactly what I'm looking for.

Basically when removing spawn from jars I plan to leave the bottom 30-40% (a couple inches of substrate) of colonized grain spawn at the bottom of the jar, case with an inch of vermiculite, and then place on a shelf to fruit. For humidity/fresh air exchange I've considered removing the lid for air but that would allow most of the humidity to escape, so for now what I'm thinking is leaving the lid disk loosely on top with a decent hole and no micropore tape/tyvek/polyfill, followed by taking the lid off and misting in the jar 1-2 times a day.

Seems simple enough, colonized mycellium, room temperature, light, fresh air, and humidity = mush right?

Any tips advice experience or links are much appreciated

MLBjammer · 07-06-09, 12:27

I don't know how good it is to leave moist vermiculite in the jar while fruiting. I know if one d.e. cases a PF cake invitro, it usually leads to contamination (that's from experience!), but the grain isn't nearly as dense as a BRF cake. I would try it without the vermiculite, but I could well be wrong. This reminds me of DukeX's whole grain rice jar tek. Anyway, good luck.

p2k1 · 07-07-09, 11:34

Im thinking about dunking the mycellium by pouring water into the jar until the mycellium is submerged, then in 6-12 hours simply pour the water back out and apply the casing layer. Simple enough, right?

I think a dunk would really help as they jars have been fully colonized for a few weeks and have started to lose moisture (noticed the effect by looking at two air holes in the lid, one had more micropore tape, other less, the one with less tape had grain under it where the mycellium looked almost stripped off it) and yellow metabolite liquid has started to pool at the bottom of them.

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07-06-09, 12:17	#1 (permalink)
p2k1 Mycotopiate Join Date: Jul 1972 Posts: 326	Quart Jar Invitro TEK Hey everyone Ive got a large amount of rye grain spawn colinized thats getting spawned outside but thought Id give a little experiment a try. Ive seen this online and have been searching but can't find exactly what I'm looking for. Basically when removing spawn from jars I plan to leave the bottom 30-40% (a couple inches of substrate) of colonized grain spawn at the bottom of the jar, case with an inch of vermiculite, and then place on a shelf to fruit. For humidity/fresh air exchange I've considered removing the lid for air but that would allow most of the humidity to escape, so for now what I'm thinking is leaving the lid disk loosely on top with a decent hole and no micropore tape/tyvek/polyfill, followed by taking the lid off and misting in the jar 1-2 times a day. Seems simple enough, colonized mycellium, room temperature, light, fresh air, and humidity = mush right? Any tips advice experience or links are much appreciated

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07-06-09, 12:27	#2 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	I don't know how good it is to leave moist vermiculite in the jar while fruiting. I know if one d.e. cases a PF cake invitro, it usually leads to contamination (that's from experience!), but the grain isn't nearly as dense as a BRF cake. I would try it without the vermiculite, but I could well be wrong. This reminds me of DukeX's whole grain rice jar tek. Anyway, good luck.

07-07-09, 11:34	#3 (permalink)
p2k1 Mycotopiate Join Date: Jul 1972 Posts: 326	Im thinking about dunking the mycellium by pouring water into the jar until the mycellium is submerged, then in 6-12 hours simply pour the water back out and apply the casing layer. Simple enough, right? I think a dunk would really help as they jars have been fully colonized for a few weeks and have started to lose moisture (noticed the effect by looking at two air holes in the lid, one had more micropore tape, other less, the one with less tape had grain under it where the mycellium looked almost stripped off it) and yellow metabolite liquid has started to pool at the bottom of them.