Can a clone sectoring on MEA because of a contam?

seymoor · 07-09-09, 12:08

Made 4 plates from a fruitbody, all looks the same cottony myc, but on a contaminated plate the myc is sectoring.
Might be some accidental spores or a piece of other myc, but is it possible for a fruitbody clone to behave differently?
Will a clone look like the same even on a different substrate or even under other circumstances?

bosshogg · 07-09-09, 13:34

not sure if i understand the question..

a pic would be very helpful..

07-10-09, 23:45

A clone isn't an isolate so it will still sector.

seymoor · 07-11-09, 04:53

Quote:

Originally Posted by Jeets

A clone isn't an isolate so it will still sector.

Can someone confirm this?

Myc · 07-11-09, 09:06

He's right.
Isolates will continue to sector

As said above.
We need a pic

seymoor · 07-11-09, 13:37

I think Jeets said exactly the opposite of what you said, Myc.

If I take a piece of a fruit body, and put it on agar, will it sector?

If I put the pieces from the same fruit body on different agars (MEA, PDA etc) will they look the same?

No pic until monday

07-11-09, 23:11

The different mediums won't make a difference in the culture sectoring. There's over 200 organisms able to be active in a single fruitbody. If you are growing from MS several sub strains can be active in a cloned fruitbody.

This is a great reason we isolate on agar. : )

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07-09-09, 12:08	#1 (permalink)
seymoor Mycophiliac Join Date: Apr 2008 Posts: 60	Can a clone sectoring on MEA because of a contam? Made 4 plates from a fruitbody, all looks the same cottony myc, but on a contaminated plate the myc is sectoring. Might be some accidental spores or a piece of other myc, but is it possible for a fruitbody clone to behave differently? Will a clone look like the same even on a different substrate or even under other circumstances?

07-09-09, 13:34	#2 (permalink)
bosshogg pro-lific lurker Join Date: Dec 2008 Posts: 733	not sure if i understand the question.. a pic would be very helpful.. __________________ 'joe schmoe super tripper might think he can eat an 8 of those like he could any other mushy and off to the loony bin,or in the corner crying for their mommy-IL

07-11-09, 09:06	#5 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,262	He's right. Isolates will continue to sector As said above. We need a pic __________________ "...we'd like to help you learn to help yourself..."

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07-10-09, 23:45	#3 (permalink)
~Jeets Guest Posts: n/a	A clone isn't an isolate so it will still sector.

07-11-09, 13:37	#6 (permalink)
seymoor Mycophiliac Join Date: Apr 2008 Posts: 60	I think Jeets said exactly the opposite of what you said, Myc. If I take a piece of a fruit body, and put it on agar, will it sector? If I put the pieces from the same fruit body on different agars (MEA, PDA etc) will they look the same? No pic until monday

07-11-09, 23:11	#7 (permalink)
~Jeets Guest Posts: n/a	The different mediums won't make a difference in the culture sectoring. There's over 200 organisms able to be active in a single fruitbody. If you are growing from MS several sub strains can be active in a cloned fruitbody. This is a great reason we isolate on agar. : )