Liquid Spawn: Slurry Spawn

[eatyualive]

this is a slight variation of 9er tek . to get you acquainted with 9er tek . read this thread below. has links to 9er tek liquid culture . this is also a slight variation of tv casualty's Slurry Tek. hence the name. just using the liquid inoculant for spawn instead of inoculating grain spawn. the part you want to look at is the 9er tek cloning part. thats it. you use an oster blender blade assembly to attach to any small mouth jar. this works for blending tissues and storing in vacutainers and syringes. or you can do this tek and instead of cutting tissue. blend up a pf cake.

http://forums.mycotopia.net/cloning/...tml#post572051 (9er Tek Details: A collection of additions)


Reasoning:
so recent failed grain battles with trich have lead me to test this out. here is the idea. if you have master pf jars from clones or plain multispore pf jars. hoping to transfer them to grain jars via grain to grain transfer using a fork or a slurry. why take the extra step of prepping grain spawn? yes it will add nutrients. your right.

but if your bulk substrate has enough nutrients to yield good flushes without the extra nutes from the grain spawn. then you are in luck my friend. here is the idea.

roughly one pf jar can be blended up using tv casualty's slurry in a hurry tek. and you can make about 150 jars of grain quarts per 1 pf jar for spawn. this will colonize in around 3 days.

http://forums.mycotopia.net/spawning...hree-days.html (TV Guide to Colonizing a Quart Jar in Three Days)

so the idea here is to skip the grain spawn all together. and go straight from pf cake blended into liquid and use anywhere from 2 pints to 2 quarts of liquid spawn to mix into your substrate. 2 pints is quite a bit of liquid spawn. and you don't want to overdo it. so it might be ideal to do this with pints. if you have a few tubs to spawn. maybe quarts are not a bad idea for multiple tubs.


Moisture Addition and not overdoing your moisture content
you will have to prep your substrate as you normally do prior to this liquid spawning. however, there is one catch here. you will have to account for moisture addition from the slurry.
so you will want to make your substrate mix slightly drier than normal. you can do this by adding some dry verm at the end after you have mixed your substrate to field capacity. or some dry horse poo can soak up more of that moisture. so right now this is just an experiment. the ratios may change. but im going to mix my normal 2 turkey tins volume of substrate per tub. im going to try 1pf cake to spawn one tub of substrate. one tub is 2 turkey tins by volume of substrate.

Recipe by volume for:1 66 quart tub or similar size tub
2 turkey tins volume of pasteurized substrate per 66 quart tub


The Slurry Per Tub is

1 colonized pf cake

1 small mouth quart jar filled with 1 pint full of distilled water (pced for 20 mins at 15psi prior)

(if you don't have a small mouth quart jar. and you only have 2 pint jars you can use these as well. you will have to cut the pf cake in half then blend it in each separate pint jar. this will be the same ratio just split in half. this will require 2 blade assemblies. im finding that less water equals more sludge consistency of the cake. so lowering your water will make it a thicker mixture. the substrate catches this better.)

illustration of small mouth pint jars and quart jars with blade assembly ready for pressure cooker. 20 mins at 15psi.



picture of a turkey tin below




Ratio by volume per tub:

1 small brick coir

6 cups of strawnet that is boiled to release the binding agent.
(it is boiled in coffee and water to add nitrogen. this isn't necessary just a trial for now. it is then strained out. no extra water is added just the water present in the strawnet)

2 quarts dry horse nugs

2 quarts dry vermiculite

this trial has supercake formula in it 1/4 cup.

this should fill two turkey tins by volume. if you don't oven pasteurize then figure out a way to squeeze out the excess moisture from your substrate or add more dry vermiculite prior to spawning to account for adding the slurry liquid. or in the end after spawning you can just squeeze out the excess moisture once you mix in the slurry.

but you have to take into account. i mix my sub to over field capacity then oven pasteurize. this loses moisture due to evaporation. so being pasteurized over field capacity allows the subs to be at field capacity once pasteurization is complete. this becomes a science at times. its easy to misjudge. if you liquid or steam pasteurize and add water after or use water to pasteurize. you have to take into account the moisture content you need has to be a little dry to account for additional liquid.

also any substrate should work using this method. im just using the ratios i prefer. just to demonstrate here.


Discussion
this is a trial experiment now. so the ratios are going to change. i will be using 2 pints of slurry liquid to my substrate for this trial run. the muddier the slurry mixture the easier the chunks catch to the substrate.

the question is. will the lack of nutrients from the grain spawn decrease yield?


Procedure:
1. setup your clean area. i use just a plain and simple trash bag. the top is taped to a lamp with tape. and everything clean is placed in it. you then use disinfectant spray to clean everything. close the bag. i then cut holes for my hands to do clean work. no airflow. this is just an example. you can see below all the pf jars and prepped sterile water in each small mouth pint and quart respectively. this also illustrates the plastic bag for semi clean work. since this is essentially like open air spawning. just be clean. not sterile.



2. loosen up your pf cake in your pf jar by lightly shaking the jar or tapping the sides of the jar. this will make it easy when you open up the pf cake and plop it into your quart. it should slide right out.

very simply open up your PF jar and scrape off the dry verm with a sterile fork or you can leave it on with no problems . then just open up your small mouth quart jar of water and plop the cake right in. try to align the tops of the jars so that the cake falls smoothly into the quart jar.



here is what it looks like after the pf cake has been flipped and dropped into the quart jar. the black blender assembly is placed over the blade assembly.



remember im using small mouth half pints and small mouth quarts so the blade assembly attaches. and you never touch anything on the outside of the jar. close the lid with the blade assembly. then place the black fitting for your oster and blend this jar up.

3. i have two blade assemblies. so its easy to do this with two jars. you may have to get another blade assembly from oster. or if you have some old blenders that happen to be oster that broke. detach the black part and the blade assembly. you blend the jars until the cake is liquefied. it may look slightly gooey. but this is ok. it will be a mycelia sludge. the sludgier the better. because when spawned it sticks to the substrate. it should look like this when your done. see where im heading with this. liquid inoculate, but ghetto version!



more illustrations of how a less water slurry will look



4. once your substrate is ready. place the plastic bag in your tub. then dump your pasteurized and cooled substrate into your bin and close the lid.



5. unscrew the black assembly from your jars. then just pour the jar or jars into your substrate evenly all around.



6. thoroughly mix your slurry into your substrate. just like spawning you want to let the material touch every part of the substrate you can. and remember the substrate is actually drier than normal so the addition of this extra moisture should factor in perfect to your moisture content. if its slightly too moist. just squeeze it out in your clean area. you may have to mix up the substrate a bit to get the moisture to distribute more evenly but it shouldn't be that bad.



the idea is speed. if it works like it does in quart jars. then the substrate should colonize in a few days. this is perfect for if your having spawn issues from contamination. it skips the grain step all together.

and this is all done open air. just clean. you can blend and prep your slurry in a shmuvbox or trashbag, or flowhood. up to you. just have your clean area setup prior to doing all of this. i skipped details about clean work thats on you.

Notes

Spawning Day: 7-10-09
ok i found a bit of trouble upon doing the spawning. the 1 quart total of liquid i used i felt was far too little to the amount of substrate. i mixed it in as thoroughly as possible but i guess thats what the experiment is for. id say double it. 2 quarts of liquid would be perfect. and you will have to make your sub even drier. but i found my sub absorbed the moisture easily and was still adequete for field capacity.

basically the pf jar was cut in half inside a plastic trash bag. then placed in each pint. the pints were blended. then the box was opened and the slurry was dumped in and spread evenly. then mixed together with gloves on.

and tv. yeah spraying it would probably work better. i think its better to even have less water and more sludge if that makes sense. more of a muddier consistency. so maybe even adding less water may work. but for large subs like this it might be ideal to try 2 pf cakes worth of mycelia to get this right. i just feel it deep down that the slurry wasn't touching the entire sub and im gonna have a half fuzzy white sub with a half uncolonized sub lol. only experimentation will tell.

Originally Posted by koldjgdaro

Let me get this straight, you take colonized jars, mix them with water into a slurry and just pour it over sub in the tub?
I wanna make a tub for the first time and I was a bit concerned about colonization times and risk of getting contams with it, but I may try this method .

yes it was done in normal spawning conditions. but at first i was wary of doing this about contams. but the substrate is not sterile it is pasteurized. so i don't see why this is any different than using clean spawn as long as your distilled water in your slurry jars are sterile. i tried to minimize air time. but the mixing took a bit longer than spawn. for now. i still like spawn better. im just waiting on some slow jars till these slurry jars i did take off. half a day in and they are showing growth!

this is just an experiment to tide over my boredom between waiting on slow spawn.

So if this tub does well and colonizes evenly in a decent amount of time. 2 more trials will be tested.

1. lower amount of water per pf cake with less substrate material

2. higher amount of water content per pf cake with less substrate material.


Day 2: 7-12-09
well growth is showing. it began the next day after spawning. now its looking like it was spawned with grain spawn with small spots. ill get some pics up once it gets colonized more.

id say lowering your water content and making the slurry like mud with about 2 PF cakes would work better. im going to attempt this next go round.. may try it with less substrate as well. this last batch was probably about 3 turkey tins full just stuffed and compacted into 2 turkey tins. pics up soon...
but imagine the potential this has if one pf cake can slurry your substrate per tub. that would mean. no more quart mason jars necessary.

also remember this is one pf cake


Day 4: 7-14-09
update. 4 days in. looking like a normal speed 4 to 5 quart sub spawning. interesting! it was looking a little fuzzy yesterday and i was worried it was cobweb . but apparently the mycelia was growing from underneath the surface. i may merge some of these posts to let you see the progression side by side.

one observation. not nearly as much condensation on the sides of the chamber during spawn run.

not to fear. the green color is strawnet.


Day 5: 7-15-09
from day 4 til day 5 there is a dramatic difference in growth.

Day 6: 7-16-09
the substrate is almost done. will be birthed on day 7. one pf cake of spawn used. took a little longer than expected. but for 1 pf cake not bad. ideally we want 3 day spawn runs using this tek. so a few more experiments will have to be done to figure out what the perfect ratio is as far as the volume of slurry goes.

Day 7: 7-17-09
Birthday! The substrate was completely done about mid way through day 6. it was then birthed at 3am on 17th. the mycelia fluffed up really nice after growth in only one day. seems like everyrhing done this trial worked out perfectly. the rhizos are everywhere. for this trial run. not bad. however, we want a 3 day spawn run like the jars. so since there was about 1 pf 1/2 pint cake worth of mycelia to about 3 turkey tins of substrate. this in turn ended up taking a bit longer. i think if it is truly 2 turkey tins volume of substrate the spawn run should be half the time it took for this trial run
here is what the tub looks like later in the day from a side view. looks stout to me.

only been 13 hours since the day 7 pic was taken above. it has definitely fluffed up nicely.




Day 11: 7-21-09
Pins! if you can't tell look near the top mid right. there were some other spots for better pics but having issues with the cam focusing.



Day 12: 7-22-09
Pins are coming along nicely. you can see in the pictures the substrate is broken from moving the tub. the amazon tub is doing nicely. i think the ape tub has cobweb. smells cobwebish. its not really colonizing. think it was a lost cause to begin with.



Day 13: 7-23-09
Pins!




Day 18 Notes: 7-28-09
harvest! fruits got a bit stringy probably due to higher co2 levels. bins had to be taken out of ideal conditions when visitors arrived. last 3 days were in the dark with little to no airflow.

Conclusion and Notes:

so far in two tests the average colonization time for 1 pf jar of slurry is 6 days. the 2nd tub is on schedule to be colonized and birthed by day 7.

from spawn till harvest was 18 days. pinning took 4 days with a strain that was well over a year old. off 3rd generation clone material 2nd flush.

the fruits were not as thick as usual. the yield so far appears to be a little less than normal. seems to be about 4z dry from eye judgement. but for 1 pf cake of spawn material. thats nice!

the fruits were just as dense as the regular fruits of this strain comparatively. more experiments will be done. life interrupted this experiment a bit and the chamber had to be taken out of ideal conditions for the last 3 days of fruiting.

dry yield was 72 grams. 2.5z. not too bad for 1 pf cake.

Side By Side

[floppypeter]

ill be keepin an eye out

[M&M420]

Sweet!!I've got three pf jars that I was wondering what I was going to use for and now I know.I'll be giving this a try real soon when they finish colonizing.

[mestophilies]

interesting, very interesting...will definately be watching eats!

[sic]

wow, one of the best things I've seen on here in awhile. thanks for sharing eats!

[lysergic]

good luck man!

[buteo]

Nice...ill be watchin too!

[PsyBearknot]

pulling up a chair to watch this.

Question

Following your theory of skipping the grain step by using the PF jar slurry would you be able to skip the PF jar slurry by using an very heavily colonized LC Jar?

Just a thought

PBK

[koldjgdaro]

Let me get this straight, you take colonized jars, mix them with water into a slurry and just pour it over sub in the tub?

I wanna make a tub for the first time and I was a bit concerned about colonization times and risk of getting contams with it, but I may try this method .

[TVCasualty]

Cool!

I'm working on a nozzle that can spray chunky slurry onto bulk to try to do this very thing but from a different angle. I wonder how many steps we can ultimately eliminate?

Originally Posted by PsyBearknot

Following your theory of skipping the grain step by using the PF jar slurry would you be able to skip the PF jar slurry by using an very heavily colonized LC Jar?

The most heavily colonized LC ever made would still only have a fraction of the mycelial mass of a colonized BRF jar due to the nature of the ingredients and how the myc grows on/in them.

However, I could see an LC having a lot more mass than most do if it were constantly aerated and the optimal nutrient levels were determined through experimentation.

I've thought about making a LC using a one-gallon glass jug with an air stone (filtered) and as much food as it can handle, then using air pressure to force the liquid through a large-bore nozzle. It might allow mass-inoculation of prepared outdoor beds, among other things. I still think an LC alone won't have as much biomass as it would need for that, so my other experiment will be to blend up a slurry and add it to a nutrified gallon LC and bubble that for a few days. Or at least that's the idea. As soon as I finish vanquishing my one and only mortal enemy (who forced me to do a total shutdown and cleanout a while back) I can get back to work. That should be later next week if all goes accoring to plan, unless I end up filling him with buckshot; in that case it'll be three weeks.

[koldjgdaro]

If everything goes right I should have a lot of jars so I may try better spawn/slurry to substrate ratio than you.

But I get your idea, cause I was thinking of something simmilar - mixing or covering the substrate with spawn in the most efficient way. Slurry sounds fine for that.

And as for the LC - I dunno, it would have to be really thick something... That would be the problem.

[Brakiska]

interesting. been reading the slurry tek lately and wanted to try it. I guess Ill try a tub and spawn a few bags of grain at the same time.

got one PE jar almost done. maybe not a few days since its PE but much faster than it would have been

will keep looking here for some results

~~

[xurzax]

I recently had the same idea of using a PF slurry to inoculate a large quantity of bulk substrate.

The best way that i was able to come up with to insure an even distribution of the mycelia laden water was to first heavily dilute it with more distilled water and then mix it into dry, sterilized vermiculite and super absorbent polymers.

Then to take the mycelium covered vermiculite/polymer and mix that into the remainder of the bulk substrate.

Basically go like PF cake -> 500ml solution -> 5000ml solution -> 10000ml vermiculite/polymer mixture -> 20,000ml verm/poly/bulk mixture

If I ever get around to trying this Ill be sure to record my results

[TVCasualty]

I've been looking at one of those cheap-ass mortar mixers at Harbor Freight. A brand new one cleaned up with iodophor might be the ticket for clean mixing of spawn into a bulk sub. Just cover the opening with a piece of plastic sheeting after adding the sub and spawn, and let it tumble for a little while, then dump into an outdoor bed.

A steam line could even be fed into it if the tub was made from either metal or HDPE (other plastics would have to be researched) and it could be an integrated bulk sub pasteurizer and spawn mixer.

I'd get a fair bit of use from one of those mixers using it to mix mortar, so me buying one is more justified than spending 150 bucks just for a spawn mixer. We'll see...

[buteo]

Lookin good

[weiliicoyote]

very cool, can't wait to see the results for this

[MrZeus]

Great work! I am fascinated by some of the teks that folks like you have invented!

[SunShine47]

This looks like a great new tek. One that i could use in the future.

[pvacant]

i've been focusing on pans only, and don't have that tough thick cubie myc, but wanna try this tek.
do ya think pan myc could, or would hold up with this tek?

[floppypeter]

i'm encouraged by the early growth, also surprised

good work my man

cant wait to see updates

[TVCasualty]

Cool! I'll try that as soon as I get a chance.

I can't wait to get back in the "lab"... Should be soon.

[eatyualive]

Originally Posted by pvacant

i've been focusing on pans only, and don't have that tough thick cubie myc, but wanna try this tek.
do ya think pan myc could, or would hold up with this tek?

good question. ive never tried anything but forking pf jars of colonized pan myeclia. i would think the mycelia would be too weak to recover. but you never know. if you have one extra pf jar to spare. try it. or you could slurry it into a spawn bag full of substrate and let it colonize like that.

definitely worth a try. with the pan dunk working so well. id not be suprised if it did work. although im skeptical.

[pvacant]

Originally Posted by eatyualive

good question. ive never tried anything but forking pf jars of colonized pan myeclia. i would think the mycelia would be too weak to recover. but you never know. if you have one extra pf jar to spare. try it. or you could slurry it into a spawn bag full of substrate and let it colonize like that.

definitely worth a try. with the pan dunk working so well. id not be suprised if it did work. although im skeptical.

i "kinda" think it'll work too. hell i'm gonna give it a go. next few days or so. work has been kicking my ass and i just don't have the energy to "work" on the hobby, but times running out and i just have too, plus i'm super excited about this tek.....so i'll let you know, eats, if pan myc can stand up to your tek.

[eatyualive]

Originally Posted by pvacant

i "kinda" think it'll work too. hell i'm gonna give it a go. next few days or so. work has been kicking my ass and i just don't have the energy to "work" on the hobby, but times running out and i just have too, plus i'm super excited about this tek.....so i'll let you know, eats, if pan myc can stand up to your tek.

excellent i don't care if you post results here either. that way everyone can see if both pans or cubes work. good luck for sure!

[koldjgdaro]

K, I'm doin the same thing!

Well done. I'm not even worried of how it's gonna go further on for you but best of luck, kudos again, eatyou.

[cap]

this strawnet stuff really must be somethin special i remember when ya 1st posted bout it a while back where do we find it tho? thx eatsy. keep us posted on progress n as always and grate thread

[eatyualive]

summitseed.com

strawnet.com

[cap]

thx.

[chimp]

[floppypeter]

pics pics pics !!!!

[eatyualive]

todays pics i merged to the top. got em in a row. will have more in the am...

its very consistent with a regular speed sub bed.

im usually getting 3 day spawn runs now. but even 6 days waiting from one pf cake aint too bad!

[eatyualive]

well the substrate is almost colonized. update above.

just a tiny few spots and by tonight it should be done. will birth tomorrow morn. its nice how the top fuzzed up too after a day.

this is cool and fun to mess with. can't wait to see some of your results. im about to do up a few more tubs of this now to test some stuff out.

[koldjgdaro]

Originally Posted by TVCasualty

Cool!

I'm working on a nozzle that can spray chunky slurry onto bulk to try to do this very thing but from a different angle. I wonder how many steps we can ultimately eliminate?



The most heavily colonized LC ever made would still only have a fraction of the mycelial mass of a colonized BRF jar due to the nature of the ingredients and how the myc grows on/in them.

However, I could see an LC having a lot more mass than most do if it were constantly aerated and the optimal nutrient levels were determined through experimentation.

I've thought about making a LC using a one-gallon glass jug with an air stone (filtered) and as much food as it can handle, then using air pressure to force the liquid through a large-bore nozzle. It might allow mass-inoculation of prepared outdoor beds, among other things. I still think an LC alone won't have as much biomass as it would need for that, so my other experiment will be to blend up a slurry and add it to a nutrified gallon LC and bubble that for a few days. Or at least that's the idea. As soon as I finish vanquishing my one and only mortal enemy (who forced me to do a total shutdown and cleanout a while back) I can get back to work. That should be later next week if all goes accoring to plan, unless I end up filling him with buckshot; in that case it'll be three weeks.

I've noticed in the lcs that the nutrients from the solution are getting absorbed by the growing mycelium, probably simple replenishing the nutrients in water would help to get the LC incredibly dense and thick.

How to do that? One idea would be to stick a syringe filled out with let's say 50% karo solution into a jar of normal 4% through a silicone port and just drop a few ml from time to time - every 3 days or so.
Moreover you could even possibly add BRF that way or whatever you want as long as you can push it out through a needle .

i love the slurry in a hurry i just pour 30 pints the other day. i accidently over poured. hope it doesnt screw anything up. im hoping to just wipe off the uncolonized parts and skate by that way. im gonna start adding verm for accidents like that.

[mystic]

On the forefront of discovery!
Eat's rocking the myc!

[eatyualive]

Originally Posted by milkman59

i love the slurry in a hurry i just pour 30 pints the other day. i accidently over poured. hope it doesnt screw anything up. im hoping to just wipe off the uncolonized parts and skate by that way. im gonna start adding verm for accidents like that.

if you use a spoon. its easy to scoop out the uncolonized parts. just watch out for green is what your looking for. ive not had contams. maybe a bit of wetspot near the bottoms of jars. but as long as you stay an inch or so away from it your fine. spoons work great.

[GadgetGuy]

This thread is off the fucking wall. You are almost making it too easy eats. Beautiful idea and I think Im going to sacrifice a few cakes just to figure this out. I think the thicker the better myself as it wouldn't fuck up your moisture content as much and would spread nearly if not just as far. I am somewhat of newb though so Im just going off my experience with WET substrates. Love the idea and will be trying it. Ill post pics if it succeeds......

[floppypeter]

wow man

looks like your on your way to success

if you could use just one slurried half pint to a tub that would be a radical savings over all the wbs maddness.

I hope it still flushes with the same vigor

wonder if any energy will be lost without the spawn?

[koldjgdaro]

I've been thinking if slurrified grain spawn would work ?
In my case it's rye.

When it comes to BRF cakes all the nutes seem to be absorbed and therefore shredding such a cake wouldn't expose extra amount of nutes that are easy to contaminate. But I'm a little worried of grain as the mycelium does not penetrate the kernels inside... Therefore shredding the grains might expose extra nutritious matter that might easily contaminate after spawning.

I hope I'm wrong about that, eatyu. Now what do you think?

[SunShine47]

This experiment in details is fanfuckingtastic!

[eatyualive]

Originally Posted by koldjgdaro

I've been thinking if slurrified grain spawn would work ?
In my case it's rye.

When it comes to BRF cakes all the nutes seem to be absorbed and therefore shredding such a cake wouldn't expose extra amount of nutes that are easy to contaminate. But I'm a little worried of grain as the mycelium does not penetrate the kernels inside... Therefore shredding the grains might expose extra nutritious matter that might easily contaminate after spawning.

I hope I'm wrong about that, eatyu. Now what do you think?

i agree that it wouldn't be good to use grain spawn unless maybe millet gets completely colonized. or maybe amaranth. just smaller type grains like this. im sure there are grains that colonize throughout. just make sure your using one. i remember seeing myc post a pic of a disected sunflower seed shell. the inside did not colonize. but you might have to test your rye. next spawn you do, leave one kernal out and cut it down the middle to see if it is colonized throughout. i might think it isn't. anyway here is what he did in this badass thread right here. if it is, then slurry away i say. i mean what harm can it do if you test one bin. even if it contams. that s ok your not wasting much. or do it in a smaller tub.

http://forums.mycotopia.net/558094-post22.html

its possible that the insides of some of the grain are not colonized. that might be more prone to contam. the brf tends to get into small enough groupings that it breaks apart easily. and the brf gets completely colonized. didn't contam after 7 days. liquid. worked out better than i could imagine at this point. gonna mess with this idea alot now.

[koldjgdaro]

Originally Posted by eatyualive

i agree that it wouldn't be good to use grain spawn unless maybe millet gets completely colonized. or maybe amaranth. just smaller type grains like this. im sure there are grains that colonize throughout. just make sure your using one. i remember seeing myc post a pic of a disected sunflower seed shell. the inside did not colonize. but you might have to test your rye. next spawn you do, leave one kernal out and cut it down the middle to see if it is colonized throughout. i might think it isn't. anyway here is what he did in this badass thread right here. if it is, then slurry away i say. i mean what harm can it do if you test one bin. even if it contams. that s ok your not wasting much. or do it in a smaller tub.

http://forums.mycotopia.net/558094-post22.html

its possible that the insides of some of the grain are not colonized. that might be more prone to contam. the brf tends to get into small enough groupings that it breaks apart easily. and the brf gets completely colonized. didn't contam after 7 days. liquid. worked out better than i could imagine at this point. gonna mess with this idea alot now.

I'll do something for sure in a couple of days. I have some whole brown rice going on as well. I may use that instead cause the myc in these jars looks extra thick and rice doesn't have a thick shell like other seeds so it may be fully penetrated...

Or another idea would be making a grain spawn slurry and letting it recover for some time but I don't have a flowhood to do all that... :/

Otherwise I gotta buy a lotta verm and brf

The tub looks quite amazing man ;].

[eatyualive]

just tested out two more slurry tubs. one being ape clone and the other being amazon.

both were used with a slightly muddier or less water slurry. it looked to workout perfectly. also the subs were a bit dry too. and the moisture added worked out perfectly.

and you do have to setup a bag to do your clean work in. it takes about 5 mins. then i find clean up to be much easier than using spawn. and it seems to be faster in the overall timing of the spawn session.

[Brakiska]

Well, its working wonders for me

It was much simplier than presumed

I removed the blade at it is supposed to

It fits perfectly on a small mouth of the jar

Lid is put back on

then blend

no holes no nothing, just need to be well sterelized

thanks for sharing this wonderful simple method, my lonely PE cake will be happy

[stimpy]

thats geniousness

[eatyualive]

the only main issue i have is that lower spawn causing lower yield. if its detrimental to your yield. then it may be better to use grain. however. if all you got is one pf cake. haha, definitely something to think about. you know how many times i find myself stuck with one pf cake and im like.....hm.....should i birth it as a cake. fuggit. ive even tossed em out before.

here is another thing. lets say this does workout. and the tub produces similar yield to what i normally get in one tub. if thats so. then that would make the more spawn equals better yield argument kinda fall apart. although i feel more spawn equals more yield anyhow lol...


now you can at the least. get a tub growing. and the yield off this tub im assuming will be greater than 1 pf cake yield. or at least hopefully lol...

its looking very aggressive and rhizo. from the last few experiments ive done. this tub seems to be doing much better than others. i think the moisture content is just right!

and even at the first spawning. i thought i added too little spawn. even when i mixed it. it seemed weird to me. like it wouldn't grow. just mix it up real good!


think about this. i can take a clone via 9er tek and inject it into 1 pf jar. then have a clone that i can slurry into quarts in around 4 days. then i get 10 quarts grown in 3 more days. so in 7 days from clone to spawning. maybe 8 at most. thats hell of a turnover. ive been doing that since tv wrote up that tek and i tried it. the speed is unbeatable. but you have many of those guys out there who will say hey its not possible to do anything without a flowhood like that. in fact, id bet if you toss this idea around you would have some really funny responses. i was tempted to post the tek. without the results first. to see what the general arguments would be from the boards. those in denial would always be in denial wether you show them all the proof or not. but it would still be funny. i do shit so ghetto and some of these guys do some real hard work here. they make hundreds of jars, hundreds of cakes. when simply put, you can get away with huge yield off 10 quarts a week spawn. if i had 10 jars of pf that can potentially do 10 tubs. as long as you rotate your pf jars. you have some awesome speed.

and when i take clones via 9er tek i generally make about 10 syringes per clone. that can last 2 years if you play it right. you can take one of those 10 colonized pf jar clones and just slurry it into 30 quarts. then boom. you just have no limit here really. depending on timing, ill generally only slurry when im desperate and my spawn is taking too long. well the only problem i have ever had with tv's tek is that everytime i do the tek. i have way too much spawn. when i don't do the tek, im usually waiting on spawn. so slurrying directly to tubs is really cool. at least its fun for me to toy with. not anymore difficult than spawning. you might have to prep some jars but its really not that bad. and this last batch i did, i used 2 pint jars. cut a pf cake in half. then plopped half in each jar. i had one blade assembly. so i blended one. placed the pint jar lid on the one i just blended. then moved the blade assembly to the other jar and basically switched lids on both. so as long as you don't touch the blade on anything you should be fine. just use quick movements when your moving the blade assembly. just doing tv's slurry tek a few times ive got this tek down to a t already. its really easier for me now than even doing g2g bc i don't have to prep all the grain jars. and i can simply use a trash bag to do my semi clean work in.



that one clone syringe i inject into 1 pf jar usually. i could just as easily inject 10 pf jars. then thats 10 tubs. and the pf jars store much easier than the quart jars. however, i like quart jars. jars run my life. but, if this eliminates prepping grain in a reasonable fashion. its way less work. imagine all the newbs that can't do grain. they could do this and have decent yield without so much work....

[TVCasualty]

Awesome. This is great stuff, and the other day I was very entertained by flipping back through that old Oss and Oeric guide to growing using agar wedge transfers to rye jars that were cased directly and fruited from the jars. I did that technique, and it was enough work to make a person decide to just grow pot instead for a few years.

A really interesting thing (to me anyway) about this and other new, fast, and high-yielding methods is that all the elements of these new teks were available back in the agar wedge transfer days...

[eatyualive]

Originally Posted by TVCasualty

Awesome. This is great stuff, and the other day I was very entertained by flipping back through that old Oss and Oeric guide to growing using agar wedge transfers to rye jars that were cased directly and fruited from the jars. I did that technique, and it was enough work to make a person decide to just grow pot instead for a few years.

A really interesting thing (to me anyway) about this and other new, fast, and high-yielding methods is that all the elements of these new teks were available back in the agar wedge transfer days...

yes the internet definitely speeds up knowledge. seeing new ideas gets you thinking. then you figure out cool new things from what you learned about that. ive got my links below that i kind of collect all the really cool methods i like. so its easier for me to look at it when need be. makes quick reference really fast. and i put it in the user notes area. so its like my personal archives!

[eatyualive]

both tubs ape and amazon. look very similar to the 2 days growth pics above. coming along nicely so far.

amazon is the more colonized one after 2 days. ape is a very old clone sitting in the fridge for a long time.

[pvacant]

Eats.....i have a half pint of pan mycelium that i'm gonna do this "slurry in a hurry" with (it is BRF verm and Poo, kind of a waylitjim type jar) and i FINALLY found my blender blade, so i'm ready to go. How much, or what ratio would i use for 1 pf style jar. i don't wanna make the bulk sub too thick for pans.
i guess what i'm asking is... how should i proceed, in your opinion, to optimise the amount of myc i have to use, and create the most...... umm...... amount of yield (if it works).
oh and the bulk sub is aged hpoo and med-course verm, and pasteurized in the oven like you do. i also have sterile H2O2, and dry sterile verm, in case i don't get the moister right.