Rye malt LC- 1 day?

cattlexing · 07-14-09, 17:09

So I was preparing some rye and poured coffee in it... and than boiled it and took that burning hot liquid rye malt extract into a syrup bottle. Than innoculated it when it cooled.
And than today, 1 day later I look at it and there is like a whole white alive looking foggy layer in the bottom. Like alot. I did put alot of spores in there. Is all that stuff good to go? Or is it too quick? Is the white stuff just some protines from the rye juice?

Soliver · 07-14-09, 22:18

Without pics and more details of your tek, it's impossible to say.

Was the bottle clean? If not, it's probably sugar residue. One day is REALLY fast
for LC, but I suppose it's not impossible.

Overall, last-minute attempts at LC's don't work so well, as sterile procedures are
very important at this stage... you may have spores growing in there, but what
else?

A syrup bottle? Odd choice. Need more info, methinks, to advise further,

soliver

GadgetGuy · 07-15-09, 00:18

Depending on how you did it I.E. glovebox, etc. that doesn't sound like the most sterile procedure I've ever heard of. Im not saying that it's contaminated but I'm guessing it just might.

cattlexing · 07-16-09, 16:10

Sterile procedures - yes
I guess it being a sypur bottle is unnecessary information
I cleaned it out 100 percent with hot water than rinsed it with alcohol.
I got a tyvec piece as a cap and a twistie tie, and alcoholed all that and set it aside... than i poured the boiling brew into the bottle and tied the twistie down real tight. I let it cool and looked at it and it seemed fairly clear, a little murky, but chill. After it cool I knocked it up with way too many spores... like2 cc. What happened was I put a glob of silocone on the tyvec to make a self sealing port. Didnt work. the hot needle melted the silocone into it, so the needle was stuck in there with with just some dripps comming out of it... eventually the silicone gave way and out popped a bunch of spore juice.

The next morning there was a healthy layer of strangeness at the bottom.

I then spawned that to some rye jars that were looking very dry, barren, sad, and lifeless... and now I have lots of white fuzzyness growin around the kernels. LCing with that super nutritious nitrogen rich (i think there was coffee in it too) pour off was really a good kick starting step.

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07-14-09, 17:09	#1 (permalink)
cattlexing Mycophiliac Join Date: Jun 2009 Posts: 82	Rye malt LC- 1 day? So I was preparing some rye and poured coffee in it... and than boiled it and took that burning hot liquid rye malt extract into a syrup bottle. Than innoculated it when it cooled. And than today, 1 day later I look at it and there is like a whole white alive looking foggy layer in the bottom. Like alot. I did put alot of spores in there. Is all that stuff good to go? Or is it too quick? Is the white stuff just some protines from the rye juice?

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07-14-09, 22:18	#2 (permalink)
Soliver Deviant Join Date: Feb 2005 Posts: 1,784	Without pics and more details of your tek, it's impossible to say. Was the bottle clean? If not, it's probably sugar residue. One day is REALLY fast for LC, but I suppose it's not impossible. Overall, last-minute attempts at LC's don't work so well, as sterile procedures are very important at this stage... you may have spores growing in there, but what else? A syrup bottle? Odd choice. Need more info, methinks, to advise further, soliver

07-15-09, 00:18	#3 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,315	Depending on how you did it I.E. glovebox, etc. that doesn't sound like the most sterile procedure I've ever heard of. Im not saying that it's contaminated but I'm guessing it just might.

07-16-09, 16:10	#4 (permalink)
cattlexing Mycophiliac Join Date: Jun 2009 Posts: 82	Sterile procedures - yes I guess it being a sypur bottle is unnecessary information I cleaned it out 100 percent with hot water than rinsed it with alcohol. I got a tyvec piece as a cap and a twistie tie, and alcoholed all that and set it aside... than i poured the boiling brew into the bottle and tied the twistie down real tight. I let it cool and looked at it and it seemed fairly clear, a little murky, but chill. After it cool I knocked it up with way too many spores... like2 cc. What happened was I put a glob of silocone on the tyvec to make a self sealing port. Didnt work. the hot needle melted the silocone into it, so the needle was stuck in there with with just some dripps comming out of it... eventually the silicone gave way and out popped a bunch of spore juice. The next morning there was a healthy layer of strangeness at the bottom. I then spawned that to some rye jars that were looking very dry, barren, sad, and lifeless... and now I have lots of white fuzzyness growin around the kernels. LCing with that super nutritious nitrogen rich (i think there was coffee in it too) pour off was really a good kick starting step.