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Old 07-24-09, 10:15   #1 (permalink)
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print / 3rd flush questions

My first 2nd flush is almost ready for harvest. If I wanted to make a syringe immediately after, could I just put the mush head directly into a sterilized jar and let it drop it spores there and then mix with sterilized, distilled water and fill up my syringe from there? If so, I guess I wouldn't need to let it rehydrate since it never really dried out. Correct?

Also, this is in a kit/bag. I did not dunk after the first flush but want to dunk for the third flush. I dont think putting it back into the bag would be a good idea so I was thinking of using a foil roasting pan and lid but the size would be different. After dunking, could I break up the compost brick (unsure what to call this at this stage) after the dunk and cover with a casing layer?

Thanks.
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Old 07-24-09, 10:19   #2 (permalink)
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you could crumble it you would want to let it recover a couple of days in incubation conditions
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Old 07-24-09, 10:27   #3 (permalink)
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so you want to put the cap, gill-side down, at the bottom of a sterilized jar to collect the dropping spores, essentially making a spore print at the bottom of the jar instead of on a piece of foil? that sounds like it would work quite well, and in fact, is a really good idea

crumbling and casing is always an option, i say go for it!
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Old 07-24-09, 10:33   #4 (permalink)
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I agree with mydarling on both points,she really knows her stuff too.
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Old 07-24-09, 10:38   #5 (permalink)
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better to suspend cap above water -
no touching to ruin it
no sticking to the glass either.
better have a decent glovebox though, imo .
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Old 07-24-09, 10:42   #6 (permalink)
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somehow i missed the part about printing but i looooove to do my prints inside jars with single injection ports
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Old 07-24-09, 10:45   #7 (permalink)
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Quote:
Originally Posted by Hippie3 View Post
better to suspend cap above water -
no touching to ruin it
no sticking to the glass either.
better have a decent glovebox though, imo .
I didn't really consider having water in the jar when making the print. My thought was to add water after it dropped the spores, then stir it around.
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Old 07-24-09, 10:49   #8 (permalink)
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Quote:
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somehow i missed the part about printing but i looooove to do my prints inside jars with single injection ports
use 2 ports and water
and you can fill syringes right out of the printed jar
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Old 07-24-09, 10:54   #9 (permalink)
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It is easier to have water in the jar for the spores to drop right in. You just have to suspend the cap above the water. Then you have a nice jar of spore water for many syringes. But just dropping the cap in the jar will work, too, but it's not guaranteed to be as clean.
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Old 07-24-09, 11:01   #10 (permalink)
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By ports, do you mean holes in the lid?

Should I add the water to the jar then sterilize? (after cooling, suspend the cap)

Would it be a good idea to use pillow stuffing in the holes, cover with foil and pc?

Would I be able to see the spores in the water to know they dropped?

Thanks again for the responses!
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Old 07-24-09, 11:09   #11 (permalink)
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ports = holes filled with silicon adhesive [re-seal as needle exits]
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Old 07-24-09, 11:15   #12 (permalink)
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I guess I'll give it a go...I hope the cap doesn't fall into the water when I moving it around a little...

Thank you.
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Old 07-24-09, 11:18   #13 (permalink)
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use a small sterile fish hook pushed up thru cap to hang it,
place a couple strands of fishing line under it to hold it.
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Old 07-24-09, 12:00   #14 (permalink)
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Quote:
Originally Posted by Hippie3 View Post
use 2 ports and water
and you can fill syringes right out of the printed jar
woops forgot the part about that being my airport cap with single injection port
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Old 07-24-09, 12:37   #15 (permalink)
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Last question of the day...

Since this would be the third flush, would it be necessary to pasteurize the casing?
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Old 07-24-09, 12:38   #16 (permalink)
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After dunking....
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