Spawning using rye berries and a dry prnt

[Hobbit Girl]

Hi,

I have a South American print I'd like to use to create some spawn. I don't have any syringes, and would like to ask if the advice below seems accurate. Basically, the main question I have is whether scraping the dry print into a sterilized rye berry jar will produce colonization at normal temp's of low 70's in a dark place.

Thanks as always, Hobbit Girl


Alternatively, you could remove the lid and scrape in some dry spores from a spore print or use some
sterile mycelium taken from an existing culture.
3. Growth
At this point, you simply place the jars in a clean, dark place and wait for permeation. You get the most
rapid growth if you shake up the grains every 3 or 4 days. This is part of the reason for using rye
berries. Rice simply won't shake up properly. It clumps. The inside of the clumps never get inoculated
and it takes weeks for the mycelium to permeate. While it is taking such a long time, it is easy for
contaminants to get the upper hand. Organic rye stays loose and allows the mycelium to rapidly
colonize.
Once colonized, you are done. The jars will stay nice and usable for 3 or 4 months if kept in a cool, dark
place

[Hippie3]

dry spores will slow germination considerably

[rags724]

ditto. If you can use tissue from an existing culture that would be the ideal option in your case.

[kcmoxtractor]

If you rehydrate your spores in the print bag for a day and then make your swipe it would probably work pretty well, same thing as a syringe.

[Hobbit Girl]

Ok. Thanks all very much for the advice. It sounds as if re-hydrating the print is the way to go. I'll look up what I can on that topic, and post additional questions if I have them.

[potatoskill]

you should really just find a syringe. they are very cheap.

[cattlexing]

crazy- so whats the diference between dry and wet spores?

[MLBjammer]

Spores germinate quicker once they are hydrated. A spore print, of course, is dry spores. If you scraped that into a substrate without hydration, it would take longer to germinate. And colonization is a race that you want the mycelium to win.

[Hippie3]

Originally Posted by potatoskill

you should really just find a syringe. they are very cheap.

if you want to help
help her do what SHE wants-
not what YOU'd do.
we do not need everyone telling folks
to do it different-
the job is to help them git 'er done THEIR way
if at all possible.

[Hippie3]

Originally Posted by cattlexing

crazy- so whats the diference between dry and wet spores?

water

[kcmoxtractor]

You will also have to do this in a glove box or under a laminar hood for a clean culture.