clone LCs not taking to grain

Getitosis · 08-05-09, 00:34

got some clone LCs that i've noccd some popcorn/poo jars with about two weeks ago and they never did anything, contam or otherwise. so today i opened them up to see what was going on. it smelled just like they did when they come from the PC, fresh. and there was very little mycellium at all in any of the jars. some around the bottoms but that was it.

LC mix is 1 tsp pure vanilla extract to 350ml of h20

jars have all been 60/40 popcorn/poo pints.

the LCs are pristine and there is crap tons of great growth in them all. none have invited contams into anything they have been used on, so what gives?

mydarling · 08-05-09, 01:00

how's your fae on those jars? what about moisture content in the grains (are they wet/dry)?

and, BTW....why did you put poop in your grain jars??

Getitosis · 08-05-09, 01:07

Quote:

Originally Posted by mydarling

how's your fae on those jars? what about moisture content in the grains (are they wet/dry)?

and, BTW....why did you put poop in your grain jars??

good fae. poly hole. tried and true.
perfect moisture content i'd say.
poo just for some oooomf. it's working and has been for a bit with straight spore injections.

hence why i ask what's going on. i've just never had this happen. doesn't make sense.

waylitjim · 08-05-09, 01:20

You probably missed the mycelium and injected mostly water in the jars.

Getitosis · 08-05-09, 01:56

Quote:

Originally Posted by waylitjim

You probably missed the mycelium and injected mostly water in the jars.

im sure that is highly possible, though my syringes were showing much growth and were used the same day i made them. still

is that a common thing though?

waylitjim · 08-05-09, 02:42

Quote:

Originally Posted by Getitosis

is that a common thing though?

It's certainly easier to get enough spores in a syringe. In a LC, the myc can clump together making it difficult to evenly distribute. This is why some peeps add broken glass to their jars. It helps to shred the mycelium and draw it out of the jar. You need a fair amount to get healthy colonization.

cattlexing · 08-05-09, 02:43

I know if the moisture content is good, than you add a syrenge full of water you're mycel filled water will suffocate in the bottom of the jar. I'd say be very careful adding water to any substrate thats not actually colonized..., or adding any LC to any grain thats not slighly dehydrated. I seemed to have made this same mistake a couple many times recently. The good news is as log as nothing contamed you should be able to colonize with a chunk of solid mycle fairly easily.

potatoskill · 08-05-09, 02:52

how many cc's of LC did you shoot per jar?

i just shot up some grain jars with LC the other day, some of the jars got huge growth right away, while others got barely any.. due to the mycelium clumping in the syringe, and sometimes you only shoot water and not the myc.. so that might be an issue like stated previously.

the other questionable thing is the poop. just question it. dont know if its a problem but.. ? u got a puzzler here!

Getitosis · 08-05-09, 14:29

thanks peeps.

just used a couple of cc's of clone LC in each jar. the suffocation idea sounds like the culprit, but i'm sure that all y'alls ideas had a part to play.

trying again with the actual piece of flesh that started the clone LCs. i got a real nice giant cluster fruit from my BHTs that i'm dying to get going.

koldjgdaro · 08-05-09, 14:39

Shake the syring before shooting each hole.

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08-05-09, 00:34	#1 (permalink)
Getitosis fallible learner Join Date: Apr 2009 Posts: 229	clone LCs not taking to grain got some clone LCs that i've noccd some popcorn/poo jars with about two weeks ago and they never did anything, contam or otherwise. so today i opened them up to see what was going on. it smelled just like they did when they come from the PC, fresh. and there was very little mycellium at all in any of the jars. some around the bottoms but that was it. LC mix is 1 tsp pure vanilla extract to 350ml of h20 jars have all been 60/40 popcorn/poo pints. the LCs are pristine and there is crap tons of great growth in them all. none have invited contams into anything they have been used on, so what gives? __________________ "tis not I that live, but that upon which I think..."--this guy

08-05-09, 01:00	#2 (permalink)
mydarling Modtastic Join Date: Jan 2008 Posts: 2,034	how's your fae on those jars? what about moisture content in the grains (are they wet/dry)? and, BTW....why did you put poop in your grain jars?? __________________ tonight your ghost will ask my ghost, who put these bodies between us?

08-05-09, 01:20	#4 (permalink)
waylitjim A Mirror Image Join Date: Sep 1972 Posts: 4,232	You probably missed the mycelium and injected mostly water in the jars. __________________ *Waylit's Exotic Roundup - a collection of sexy threads*

08-05-09, 14:29	#9 (permalink)
Getitosis fallible learner Join Date: Apr 2009 Posts: 229	thanks peeps. just used a couple of cc's of clone LC in each jar. the suffocation idea sounds like the culprit, but i'm sure that all y'alls ideas had a part to play. trying again with the actual piece of flesh that started the clone LCs. i got a real nice giant cluster fruit from my BHTs that i'm dying to get going. __________________ "tis not I that live, but that upon which I think..."--this guy

08-05-09, 02:43	#7 (permalink)
cattlexing Mycophiliac Join Date: Jun 2009 Posts: 82	I know if the moisture content is good, than you add a syrenge full of water you're mycel filled water will suffocate in the bottom of the jar. I'd say be very careful adding water to any substrate thats not actually colonized..., or adding any LC to any grain thats not slighly dehydrated. I seemed to have made this same mistake a couple many times recently. The good news is as log as nothing contamed you should be able to colonize with a chunk of solid mycle fairly easily.

08-05-09, 02:52	#8 (permalink)
potatoskill Mycotopiate Join Date: Dec 2006 Posts: 200	how many cc's of LC did you shoot per jar? i just shot up some grain jars with LC the other day, some of the jars got huge growth right away, while others got barely any.. due to the mycelium clumping in the syringe, and sometimes you only shoot water and not the myc.. so that might be an issue like stated previously. the other questionable thing is the poop. just question it. dont know if its a problem but.. ? u got a puzzler here!

08-05-09, 14:39	#10 (permalink)
koldjgdaro Psychonaut Join Date: Sep 2007 Posts: 747	Shake the syring before shooting each hole. __________________ To fathom Hell or soar angelic, just take a pinch of psychedelic

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