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Old 08-16-09, 03:32   #1 (permalink)
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Harvest advice

FOAF returned from his 2 week trip, finding that his friend had harvested five or six of the biggest specimens from the bag he'd left in his care. The substrate was left in the re-sealed bag and looked like it was either contaminated or there was significant bluing of the mycelium and the remaining mushrooms. He did a q-tip swab to test, and no color transferred to the cotton, so his opinion is that it was pretty much bluing. The bag wasn't in the best shape, so he decided to finish the harvest. LOTS of aborts, as the pics below will show.

Question is, how small is too small? The tiniest ones look no bigger than a maggot. Are they worth saving? One mushroom he almost threw in the trash because it was beginning to get slimy on the cap, but he decided to try drying it out. All these are being lightly fanned for a day or so before being placed in a plastic tub with damp-rid to completely dehydrate them.

Some of the substrate is clinging to a few of the mushrooms. Any problem with eating a few flakes of vermiculite?

Also, the cake is in the fridge soaking in a water/bleach solution (about 1.5 to 2 tablespoons in a half gallon of water) to counteract contams. He plans on placing the cake in a fruiting chamber with verm and perlite to try for further flushes. He doesn't have any poo to use right now, so this is what he deems his best bet for now.

Advice, please?
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Old 08-16-09, 04:22   #2 (permalink)
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Those little aborts add up. Save as many as you can--shrooms are shrooms whether tiny or enormous.

Inevitably you will have a little vermiculite on your fruits--unless you don't use vermiculite. Get off as much of it as you can, but a flake or two never killed me.

If you could spawn your cake to something bulk, you would get a bigger yield. If you can't find poo, you could try just some coir (reptile bedding in a pet store). Or just leave the cake alone--often a second flush is better than the first.

And there is no need for bleach IMO in your dunk water unless you are fighting a contam.

You got some shrooms, which is the main point. Good luck.
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Old 08-16-09, 07:45   #3 (permalink)
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I always use a scalpel to peel off anything that is not a mushroom tissue.

Just like with wild edibles of any kind - clean them up nicely.

It won't kill anybody but it's a shame to show to ppl mushrooms that have poo on them or something else...
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Old 08-16-09, 10:14   #4 (permalink)
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Careful with bleach! You only want to put mycelium into bleach water for a short time, called a dip. Then you might want to rinse or do a plain water dunk.

I've killed a couple of projects because I didn't know how to use bleach, which is a couple projects too many.
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Old 08-16-09, 10:25   #5 (permalink)
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Quote:
Originally Posted by Sidestreet Getaway View Post
Careful with bleach! You only want to put mycelium into bleach water for a short time, called a dip. Then you might want to rinse or do a plain water dunk.

I've killed a couple of projects because I didn't know how to use bleach, which is a couple projects too many.
Oops, probably too late then, it's been in the fridge all night.
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Old 08-16-09, 11:34   #6 (permalink)
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That is a very high ratio you mentioned for your bleach dunk. You want something like 200:1 for a bleach dip. I wouldn't throw it out, as mycelium is a tough customer, so you never know.

But just remember: only use bleach if you are trying to save something. It works sometimes, depending on the severity of the contamination.

Good luck!
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Old 08-16-09, 12:10   #7 (permalink)
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Quote:
Originally Posted by MLBjammer View Post
That is a very high ratio you mentioned for your bleach dunk. You want something like 200:1 for a bleach dip. I wouldn't throw it out, as mycelium is a tough customer, so you never know.

But just remember: only use bleach if you are trying to save something. It works sometimes, depending on the severity of the contamination.

Good luck!
200:1 sounds like tap water, lol....

Ok, so should FOAF just use vermiculite on top and bottom of cake and place in FC with perlite, or is it better to spawn? He has no poo, and has never tried coir, but has verm/coir and straight verm (very fine) on hand.
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Old 08-16-09, 12:11   #8 (permalink)
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That is a very high ratio of bleach to water. If you haven't killed the mycelium, you will likely get really funky mutated shrooms the next flush if it flushes at all. I would recommend starting over at this point. At least you got a few edible boomers out of the grow.
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Old 08-16-09, 12:40   #9 (permalink)
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Quote:
Originally Posted by bozefonce View Post
200:1 sounds like tap water, lol....

Ok, so should FOAF just use vermiculite on top and bottom of cake and place in FC with perlite, or is it better to spawn? He has no poo, and has never tried coir, but has verm/coir and straight verm (very fine) on hand.
Bleach is damn potent stuff. Read this: http://archives.mycotopia.net/discus...tml?1043192825
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Old 08-18-09, 08:16   #10 (permalink)
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Quote:
Originally Posted by TastyBeverage View Post
That is a very high ratio of bleach to water. If you haven't killed the mycelium, you will likely get really funky mutated shrooms the next flush if it flushes at all. I would recommend starting over at this point. At least you got a few edible boomers out of the grow.
FOAF put the cake in the FC with wet perlite a couple days ago just to see what happens. Rather give it a try than to waste a potential flush. Worst that could happen is nothing. Well, after about 48 hours the cake has begun to fuzz over again with new mycelium, and a few knots beginning to form. This might turn out ok after all! We'll post pics when things get interesting. Apparently mycelium is tougher than we all thought. Hopefully the shrooms don't mutate like you said, but we shall see.

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Old 08-18-09, 08:39   #11 (permalink)
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Yeah a 1:100 solution still needs to be rinsed off when used as a sanitizer in brewing. I think the no rinse concentration was about a 1:250 dilution for 15-30 minutes.

Last edited by essentrik1; 08-18-09 at 08:51. Reason: should use the same units for dilution
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Old 08-18-09, 09:44   #12 (permalink)
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Quote:
Originally Posted by bozefonce View Post
FOAF put the cake in the FC with wet perlite a couple days ago just to see what happens. Rather give it a try than to waste a potential flush. Worst that could happen is nothing. Well, after about 48 hours the cake has begun to fuzz over again with new mycelium, and a few knots beginning to form. This might turn out ok after all! We'll post pics when things get interesting. Apparently mycelium is tougher than we all thought. Hopefully the shrooms don't mutate like you said, but we shall see.

Good vibes your way!
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Old 08-19-09, 01:35   #13 (permalink)
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Quote:
Originally Posted by MLBjammer View Post
Good vibes your way!
Thanks, MLB, and thanks to all for the advice, vibes and support. Here's a couple pics of the cake in the FC. The cake looks a bit ragged along what once was the top surface when it was inside the bag. The myc looks pretty good, should see primordia and pins soon in some areas.
The dark spots in some places are remnants of noobish harvesting techniques from the first flush. As far as FOAF can tell there's no sign of contams.
Keep in mind this was following a very long soak in fairly strong chlorine water solution, something like 15 to 18 hours.
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Old 08-21-09, 07:48   #14 (permalink)
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Yeah, that myc looks happy. I'm glad the bleach didn't hurt it.
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Old 08-21-09, 07:57   #15 (permalink)
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Excellent! There should be fruiting soon. Glad to help, my friend.
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Old 08-22-09, 11:32   #16 (permalink)
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GOT PINS?

PIN DANCE, WOOT!!!!
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Old 08-22-09, 13:09   #17 (permalink)
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Excellent, especially after your bleach experience. Congrats.
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Old 08-22-09, 21:16   #18 (permalink)
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Glad the bleach didnt ruin your project. I've had too much bleach solution in perlite in tub and the cakes didn't fruit at all (except one), and that was just from vapors.
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Old 08-22-09, 21:25   #19 (permalink)
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Hooray those are fat ones too
glad to hear it worked out
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Old 08-22-09, 21:44   #20 (permalink)
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Glad to hear it worked out! I wouldn't do that with bleach again, and also, you really shouldn't bleach dip unless you have a very obvious contam. It is really a worse case scenario kind of thing. You said you just harvested your first flush, so the cake was likely not even contaminated anyway. Mycelium is pretty contam resistant, but it's strength diminishes with its nutrients and with time. I have only had contams on spent cakes, never during normal flushing.


This sounds like your first grow, is it? My first grow I thought some bruising on my cakes was a contam and threw 6 out of 12 cakes out only to find out later that they were likely just fine and a little bruised. I think this is a common mistake for noobs because contams is the biggest enemy of a mushroom grower and they are quick to overreact. I sure as hell did.


Also, SAVE THOSE DAMN ABORTS! Don't mean to offend anyone with all caps hehe but really, those things add up! What I do is simply put the fresh aborts/small pins on a paper plate to dry out in the open air. This works fine, although the big ones require a fan and/or a drying agent like NaOH (lye). And about that slimy one you said you almost threw out, don't throw those out ever, they will be normal once dried; you won't even be able to tell the difference.


Good luck, sending good grow vibes your way!
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Old 08-24-09, 22:52   #21 (permalink)
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Good luck, sending good grow vibes your way!
Thanks for all the feedback and good vibes, Ishkaboo, and to all the others who've posted in this thread, I really appreciate it.

Here are some pics taken just a couple minutes ago, lookin' purdy! I think we know which ones will be harvested for cloning...

Definitely gonna get prints, too.
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Old 08-24-09, 23:02   #22 (permalink)
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Way to go. Sending vibes for a big flush.
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Old 08-24-09, 23:13   #23 (permalink)
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Old 08-25-09, 10:07   #24 (permalink)
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I like your FC setup bozefonce.
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Old 08-25-09, 22:24   #25 (permalink)
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I like your FC setup bozefonce.
Thanks man, just following those who have gone before!
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Old 08-25-09, 22:39   #26 (permalink)
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That is a very high ratio of bleach to water. If you haven't killed the mycelium, you will likely get really funky mutated shrooms the next flush ...
this worried me as well. looks pretty good though. still not sure if i would print or clone yet.. give them a little more time and see how they mature.

nice work!

do we have an archive for bleach dunking? if so this may make a good addition.

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Old 08-25-09, 22:49   #27 (permalink)
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this worried me as well. looks pretty good though. still not sure if i would print or clone yet.. give them a little more time and see how they mature.
Gonna try and do both, looks like some likely candidates for cloning, and a print or two will also come in handy to preserve the Z strain for future use. FOAF has an order of blank syringes coming, which will basically set him up for self sufficiency along with all the other crap he's been buying up.

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do we have an archive for bleach dunking? if so this may make a good addition.

LOL... on this point I'm not sure if it's the best lesson for the noobs to follow. If anything it's some isolated evidence that SOME mycelia can handle higher concentrations of bleach. Still wouldn't recommend it in practice. Might give hope to those who have made the same mistake.... I'll let the mods decide whether this is archive-worthy.

Thanks for all the support!

More pics will follow, looks like mad pins in there now. Gonna be a pretty decent flush if things go as indicated at this point.
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Old 08-25-09, 22:59   #28 (permalink)
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Keep all those tiny little aborts for yourself.... will be worth it for sure. My favorite to eat.
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Old 08-26-09, 23:03   #29 (permalink)
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Ooooh yeah!

A few new pics, some nice, fat shrooms comin' up and a load of pins all around. 3 words: MIST THE CAKE!

The hygrometer's reading a bit low 'cause it was outside the FC for a few minutes, but it's been reading in the '90s most of the time. Too much humidity fries the battery too quickly and kills the LCD till it dries, so it has to be left out in the dry air for a bit. Seems like the boomers soak up the water pretty quickly, and the mycelium a little less quickly, but the pins are coming around nicely. Some bluing of the mycelium, it seems to come and go a little, but it's mostly on the one side. The stems of the biggest shrooms show a tad blue also, don't know why, 'cause they're not being handled at all. Does fatness cause bluing? I dunno.
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Old 08-26-09, 23:12   #30 (permalink)
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A few new pics, some nice, fat shrooms comin' up and a load of pins all around. 3 words: MIST THE CAKE!

The hygrometer's reading a bit low 'cause it was outside the FC for a few minutes, but it's been reading in the '90s most of the time. Too much humidity fries the battery too quickly and kills the LCD till it dries, so it has to be left out in the dry air for a bit. Seems like the boomers soak up the water pretty quickly, and the mycelium a little less quickly, but the pins are coming around nicely. Some bluing of the mycelium, it seems to come and go a little, but it's mostly on the one side. The stems of the biggest shrooms show a tad blue also, don't know why, 'cause they're not being handled at all. Does fatness cause bluing? I dunno.
heh. it might with those. they are fat. i'm not real experienced with this strain though. maybe someone else will chime in who knows more.

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Old 08-27-09, 23:17   #31 (permalink)
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Ooohh, Aaaahhh!!

Sorry about the oohing and aahing, just that it's the first time I've experienced freely fruiting cubies.

Here are the latest pics, wondering if FOAF is really ready to try printing.
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Old 08-27-09, 23:30   #32 (permalink)
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Looking Good!
Good Luck with the rest of the grow!
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Old 08-28-09, 12:12   #33 (permalink)
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Originally Posted by bozefonce View Post
Here are the latest pics, wondering if FOAF is really ready to try printing.
Go ahead and try printing, dont have noting to lose. If you dont need to store the print just drop the opened cap in a clean jar, then when it drops dark thick print on bottom, squirt full syringe of h2o in it, swril it around and suck it up. Those will eat through your next substrate because theyro so fresh.
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Old 08-29-09, 00:55   #34 (permalink)
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LOL... on this point I'm not sure if it's the best lesson for the noobs to follow. If anything it's some isolated evidence that SOME mycelia can handle higher concentrations of bleach. Still wouldn't recommend it in practice. Might give hope to those who have made the same mistake.... I'll let the mods decide whether this is archive-worthy.

Thanks for all the support!
you'll see. it's just a matter of time before you become consumed.. becoming one of us myco-freaks. then you'll find everything interesting. even if it's slightly out of the norm.

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Old 08-29-09, 00:56   #35 (permalink)
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Sorry about the oohing and aahing, just that it's the first time I've experienced freely fruiting cubies.

Here are the latest pics, wondering if FOAF is really ready to try printing.
no.. i was wrong. it already happened

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Old 08-29-09, 01:37   #36 (permalink)
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Question, no disrespect intended either bozefonce, but could that rusty rack that your cake is setting on in your FC cause problems in the way of contaminants down the road?
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Old 08-29-09, 01:43   #37 (permalink)
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no.. i was wrong. it already happened

I have no idea what you're talking about.
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Old 08-29-09, 01:45   #38 (permalink)
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Question, no disrespect intended either bozefonce, but could that rusty rack that your cake is setting on in your FC cause problems in the way of contaminants down the road?
Entirely possible, it's been bothering me too. Aside from the rust getting in the perlite and discoloring the cake, I don't think the iron oxide necessarily does any harm, but it's messy and could allow for harboring of contams. Been looking for a plastic rack that would serve the same purpose, and also considering some kind of waterproof coating for the rack. Anybody got some suggestions?
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Old 08-29-09, 03:28   #39 (permalink)
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I notcied that your temps are at 80 for fruiting. IMO, this is way too high. I prefer to incubate at 80 and fruit at 70. Incubating at 80 drops the risk of contams a significant amount without sacrificing too much colonization time, and fruiting at 70 gives stronger mushrooms, IME.

Other than that, which is just preference, that's not a bad job. The one on the side looks like it's trying to hug somebody.
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Old 08-29-09, 09:18   #40 (permalink)
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I notcied that your temps are at 80 for fruiting. IMO, this is way too high. I prefer to incubate at 80 and fruit at 70. Incubating at 80 drops the risk of contams a significant amount without sacrificing too much colonization time, and fruiting at 70 gives stronger mushrooms, IME.

Other than that, which is just preference, that's not a bad job. The one on the side looks like it's trying to hug somebody.
Yeah, the ambient temps in the house are slightly high by choice, FOAF prefers warmer temps for personal comfort and lower power bills. Also, he has several projects going on now, some bags colonizing, and the one cake that's fruiting. Actual temps fluctuate between 77 and 80 based on time of day, which just barely splits the difference between ideal fruiting and ideal colonization temps. Hopefully the cubies can go with slightly warm and still fruit fairly well.
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Old 08-29-09, 11:40   #41 (permalink)
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Fruiting at 80F works fine.

But contams grow fast at 80F. Keep an eye out and remove anything that gets nasty quickly.
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Old 08-29-09, 12:39   #42 (permalink)
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So... Has swim sampled any of the aborts from the first flush? There looks to be enough there to have one helluva sampling.
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